Định lượng photometric cạnh tranh enzyme miễn dịch cho fullerene C60 và các dẫn xuất của nó bằng cách sử dụng một hợp chất fullerene liên hợp với peroxidase củ cải đường

Microchimica Acta - Tập 183 - Trang 211-217 - 2015
Olga D. Hendrickson1, Natalya I. Smirnova1, Anatoly V. Zherdev1, Peter G. Sveshnikov2, Boris B. Dzantiev1
1A.N. Bach Institute of Biochemistry, Federal Research Centre “Fundamentals of Biotechnology”, Russian Academy of Sciences, Moscow, Russia
2Russian Research Center of Molecular Diagnostics and Therapy, Moscow, Russia

Tóm tắt

Bài báo mô tả một phương pháp thử nghiệm miễn dịch enzyme vi mạch loại ELISA nhạy cảm cao một bước cho fullerene C60 và các dẫn xuất của nó. Các kháng thể đơn dòng chống fullerene và một hợp chất giữa fullerene và peroxidase củ cải đường đã được sử dụng làm thuốc thử đặc hiệu. Một thử nghiệm ELISA cạnh tranh trực tiếp được thực hiện dựa trên các kháng thể được cố định trong giếng của đĩa vi titer, một kháng nguyên gắn nhãn peroxidase, và việc phát hiện thông qua thuốc nhuộm hình thành từ 3,3′,5,5′-tetramethylbenzidine và hydrogen peroxide. Cả fullerene C60 nguyên chất và các dạng tan trong nước của nó đều có thể được xác định. Giới hạn phát hiện là 1,5 ng∙mL−1 cho fullerene C60 và từ 0,1 đến 1,3 ng∙mL−1 cho các dẫn xuất của nó. Định dạng ELISA này cho phép giảm gần như một nửa thời gian cần thiết cho phép thử nghiệm so với sơ đồ gián tiếp với các kháng thể gắn nhãn.

Từ khóa

#fullerene C60 #ELISA #enzyme miễn dịch #kháng thể đơn dòng #peroxidase củ cải đường

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