Comparison of the sensitivity of a 24 h-shell vial assay, and conventional tube culture, in the isolation of Herpes simplex virus – 1 from corneal scrapings
Tóm tắt
Herpes simplex keratitis is a sight threatening ocular infection. A rapid and specific diagnosis is essential for the institution of specific antiviral therapy and to avoid complications that can arise from misdiagnosis and inappropriate treatment. Though a variety of techniques are available, isolation of Herpes simplex virus 1 (HSV-1) in culture provides the most reliable and specific method, and is considered as the gold standard in laboratory diagnosis of herpes simplex keratitis. We report a comparative study of the sensitivity of a 24 h-shell vial assay and conventional tube culture in the isolation of HSV-1 from corneal scrapings. A total of 74 corneal scrapings obtained from 74 patients with a clinical suspicion of herpes simplex keratitis submitted for the isolation of HSV-1, were simultaneously inoculated into shell vial and tube cultures employing the vero cell line. Shell vial and tube cultures were terminated at 24 h and fifth day respectively. Isolation of HSV-1 was confirmed employing an indirect immunofluorescence assay. HSV-1 was isolated from 24/74 (32.4%) specimens employing both the methods. Sensitivity of both the techniques were found to be similar (20/24, 83.3%) (P = 1.0). A 24 h-shell vial assay is a rapid alternative technique in comparison to the time consuming conventional tube cultures for the isolation of HSV-1, especially from corneal scrapings for the laboratory diagnosis of herpes simplex keratitis.
Tài liệu tham khảo
Yamamoto S, Shimomura Y, Kinoshita S, Nishida K, Yamamoto R, Tano Y: Detection of herpes simplex virus DNA in human tear film by polymerase chain reaction. Am J Ophthalmol. 1994, 117: 160-163.
Holland EJ, Schwartz GS: Classification of herpes simplex virus keratitis. Cornea. 1999, 18: 144-154.
Espy MJ, Uhl JR, Mitchell PS, Thorvilson JN, Wold AD, Smith TF: Diagnosis of herpes simplex virus infection in the clinical laboratory by Light Cycler PCR. J Clin Microbiol. 2000, 38: 795-799.
Kowalski RP, Gordon YJ: Evaluation of immunologic tests for the detection of ocular herpes simplex virus. Ophthalmology. 1989, 96: 1583-1586.
Thiel MA, Bossart W, Bernauer W: Improved impression cytology techniques for the immunopathological diagnosis of superficial viral infections. Br J Ophthalmol. 1997, 81: 984-988.
Johnson FB, Luker G, Chow C: Comparison of shell vial culture and the suspension infection method for the rapid detection of herpes simplex viruses. Diag Microbiol Infect Dis. 1993, 16: 61-66. 10.1016/0732-8893(93)90131-P.
Gleaves CA, Smith TF, Shuster EA, Pearson GR: Comparison of standard tube and shell vial cell culturetechniques for the deletion of cytomegalovirus in clinical specimens. J Clin Microbiol. 1985, 21: 217-221.
Walpita P, Darougar S, Thaker U: A rapid and sensitive culture test for detecting herpes simplex virus from the. Br J Ophthalmol. 1985, 69: 637-639.
Espy MJ, Wold AD, Jesperson DJ, Jones MF, Smith TF: Comparison of shell vials and conventional tubes seeded with rhabdomyosarcoma and MRC-5 cells for the rapid detection of herpes simplex virus. J Clin Microbiol. 1991, 29: 2701-2713.
Wiedbrauk DL, Johnson SL: Manual of clinical virology,. New York, Raven press Ltd. 1993
Johnson FB, Visick EM: A rapid culture alternative to the shell vial method for the edetction of herpes simplex virus. Diag Microbiol Infect Dis. 1992, 15: 673-678. 10.1016/0732-8893(92)90070-A.
Zaitseva NS, Muraveiva TV, Vinogradava VI, Shubladze AK, Maevskaya TM, kasparov AA, Rzhechitskaya OV: Comparative evaluation of methods for laboratory diagnosis of herpetic eye disease. Am J Ophthalmol. 1973, 75: 997-1003.
Kaye SB, Baker K, Bonshek K, Maseruka H, Grinfeld E, Tullo A, Easty DL, Hart CA: Human herpes viruses in the cornea. Br J Ophthalmol. 2000, 84: 563-571. 10.1136/bjo.84.6.563.
Mori Y, Shimomura Y, Inoue Y, Kinoshita S: Susceptibility of cultural rabbit corneal epithelial cells to various herpes simplex virus isolates. Jpn J Ophthalmol. 1996, 40: 367-370.
Araki-Sasaki K, Ohashi Y, Sasabe T, Hayashi K, Watanabe H, Tano Y, Handa H: An SV40 immortalized human corneal epithelial cell line and its characterization. Invest Ophthalmol Vis Sci. 1995, 36: 614-621.
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