Comparison of pH measurements made using <sup>31</sup>P NMR and a fibreoptic pH meter

NMR in Biomedicine - Tập 5 Số 6 - Trang 360-363 - 1992
Rama Jayasundar1, Laurance D. Hall2, N M Bleehen1
1Medical Research Council Clinical Oncology and Radiotherapeutics Unit, Hills Road, Cambridge CB2 2QH, UK
2Herchel Smith Laboratory for Medicinal Chemistry, Cambridge University School of Clinical Medicine, University Forvie Site, Robinson Way, Cambridge CB2 2PZ, UK

Tóm tắt

AbstractThe objective of this study was to compare pH measurements made in biological samples using 31P NMR (pHNMR) with those made with a novel, dye‐based fibreoptic pH measurement system (pHF), which is compatible with use in electromagnetic fields without field perturbation. Using protein‐free model solutions, pHNMR was calibrated against pHF, giving a correlation coefficient of 0.969 and a mean difference (± SD) between pHNMR and pHF of 0.037±0.054 over the pH range 6.8–7.7. Further calibration of pHNMR with pHF was carried out for human red blood lysates and then pHNMR was compared with pHF for whole, packed red blood cells over the pH range 7.0–7.8. Values for pHNMR, the intracellular pH, were consistently lower than for pHF, the extracellular pH, by a mean (±SD) of 0.15±0.02 units. A close correlation of extracellular pHNMR with pHF was demonstrated for a blood sample exhibiting two Pi peaks, over the pH range 7.03–7.71. We conclude that concurrent use of NMR and the fibreoptic pH meter provides a reliable method of simultaneous measurement of intracellular and extracellular pH in biological systems.

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NMR in Biomedicine

Tập 5 Số 6

360-363

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