Comparison of four routinely used methods for assessing root colonization by arbuscular mycorrhizal fungi

Botany - Tập 90 Số 11 - Trang 1073-1083 - 2012
Xueguang Sun1, Ming Tang1
1College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, PR China

Tóm tắt

Quantifying the proportion of roots colonized by arbuscular mycorrhizal fungi (AMF) is routine work for researchers conducting AMF studies. However, in practice, the methods are always misused, with their adaptability to different conditions neglected. In this study, four frequently used methods (root segment ±, root segment estimation, grid-line intersect, and magnified intersections) were evaluated and compared. Using the light microscopy based staining technique, we assessed AMF colonization of the roots of five plant species (Trifolium repens Linn., Zea mays Linn., Robinia pseudoacacia Linn., Populus simonii Carr., and Caragana korshinskii Kom.). The results revealed that a root length of at least 150 cm (rather than the usual 30 or 50 cm or 100 to 150 intersections generally used when following these four methods) should be examined to represent a single root sample whatever the method used. All four methods had good reproducibility, even though there was a high level of divergence among the results obtained using the different methods to assess the same root sample. We concluded that when assessing the AMF colonization of roots from the same species, all methods except the root segment ± method can be used; however, when assessing root samples from different species, the root segment estimation and magnified intersections methods give more reliable results. We suggest that the root segment ± method is an effective method for revealing the uniformity of AMF distributed in host roots of a certain length.

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Tài liệu tham khảo

10.1111/j.1469-8137.1978.tb01631.x

10.2136/sssaj1977.03615995004100030026x

10.1007/s00572-006-0045-x

10.1111/j.1469-8137.1977.tb04832.x

10.2307/3792863

10.2136/sssaj1981.03615995004500050009x

10.1111/j.1469-8137.1981.tb01690.x

10.1139/b84-290

Brundrett, M., Bougher, N., Dell, B., Grove, T., and Malajczuk, N. 1996. Roots and VAM fungi.InWorking with mycorrhizas in forestry and agriculture. Australian Centre for International Agricultural Research, Canberra, Australia. pp. 184–187.

10.1016/0038-0717(82)90052-9

10.1111/j.1469-8137.2004.01075.x

10.1007/s00572-009-0231-8

10.1016/0038-0717(94)90263-1

10.1046/j.1440-1703.2000.00329.x

10.1016/j.soilbio.2010.12.010

10.1111/j.1469-8137.1980.tb04556.x

10.1016/S0953-7562(09)80005-1

10.1016/j.envpol.2007.01.024

10.1046/j.0028-646X.2001.00312.x

10.1016/S0007-1536(70)80123-1

10.1046/j.1365-294x.1999.00604.x

10.1016/0038-0717(77)90055-4

10.1139/b87-059

Khaosaad T., 2010, Physiol. Plant., 140, 238

Kormanik, P.P., and McGraw, A.C. 1982. Quantification of vesicular–arbuscular mycorrhizae in plant roots.InMethods and principles of mycorrhizal research.Edited byN.C. Schenck. The American Phytopathological Society, St. Paul, Minnesota. pp. 37–45.

10.1139/m80-090

10.1016/S0953-7562(89)80195-9

10.1111/j.1469-8137.1983.tb02721.x

10.1111/j.1469-8137.1990.tb00476.x

10.1007/s00425-009-1057-5

10.1111/j.1469-8137.1973.tb02017.x

10.1016/j.soilbio.2009.02.027

10.2307/2401670

Nicholson, T.H. 1955. The mycotrophic habit in grasses. Ph.D. thesis, University of Nottingham, Nottingham, UK.

10.1007/s11557-008-0579-8

10.1111/j.1574-6941.1999.tb00621.x

10.1016/S0953-7562(09)80723-5

10.1046/j.1469-8137.2003.00810.x

10.1016/S0007-1536(70)80110-3

10.1111/j.1469-8137.1976.tb04657.x

10.1007/BF02803144

10.1016/j.pedobi.2008.02.002

10.1111/j.1469-8137.1977.tb04829.x

Smith, S.E., Read, D.J., and Harley, J.L. 1997. Mycorrhizal symbiosis. 2nd ed. Academic Press, New York.

Sparling, G.P., and Tinker, P.B. 1975. Mycorrhizas in Pennine grassland.InEndomycorrhizas.Edited byF.E. Sanders, B. Mosse, and P.B. Tinker. Academic Press, London. pp. 545–560.

10.1016/j.sajb.2007.11.004

10.2307/3792884

10.1016/j.soilbio.2009.07.026

Vierheilig H., 1998, Appl. Environ. Microbiol., 64, 5004, 10.1128/AEM.64.12.5004-5007.1998

Vierheilig H., 2005, Physiol. Plant., 125, 393, 10.1111/j.1399-3054.2005.00564.x

10.1097/00010694-199609000-00003

Wright S.F., 1987, Appl. Environ. Microbiol., 53, 2222, 10.1128/AEM.53.9.2222-2225.1987

10.1007/s11703-010-0096-9

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Botany

Tập 90 Số 11

1073-1083

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