Comparison of four purification methods to purify cysteine protease from Asian pear fruit (Pyrus pyrifolia)

Ajila, 2007, Valuable components of raw and ripe peels from two Indian mango varieties, Food Chem., 102, 1006, 10.1016/j.foodchem.2006.06.036 Arshad, 2014, Bromelain: an overview of industrial application and purification strategies, Appl. Microbiol. Biotechnol., 98, 7283, 10.1007/s00253-014-5889-y Babu, 2008, Liquid–liquid extraction of bromelain and polyphenol oxidase using aqueous two-phase system, Chem. Eng. Process., 47, 83, 10.1016/j.cep.2007.08.006 Bai, 2000, The properties of proteolytic enzymes in fruits (pear, kiwifruit, fig, pineapple and papaya), Korean J. Food Cook. Sci., 16, 363 Bradford, 1976, A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein dye-binding, Anal. Biochem., 72, 248, 10.1016/0003-2697(76)90527-3 Chaiwut, 2010, Extraction and three-phase partitioning behavior of proteases from papaya peels, Process. Biochem., 45, 1172, 10.1016/j.procbio.2010.03.019 Chalabi, 2014, Proteolytic activities of kiwifruit actinidin (Actinidia deliciosa cv. Hayward) on different fibrous and globular proteins: a comparative study of actinidin with papain, Appl. Biochem. Biotechnol., 172, 4025, 10.1007/s12010-014-0812-7 Choe, 1996, A study on utilization as meat tenderizer from Korean pear protease, Korean, J. Food Sci. Anim. Resour., 16, 89 Choe, 1996, A new protease in Korean pears as meat tenderizer, Anim. Sci. Technol., 67, 43 Cortez, 1999, Xylanase and b-xylosidase separation by fractional precipitation, Process. Biochem., 35, 277, 10.1016/S0032-9592(99)00064-3 Costa, 2014, A new procedure based on column chromatography to purify bromelain by ion exchange plus gel filtration chromatographies, Ind. Crop. Prod., 59, 163, 10.1016/j.indcrop.2014.04.042 Cui, 2005, Analyses of arbutin and chlorogenic acid, the major phenolic constituents in Oriental pear, J. Agric. Food Chem., 53, 3882, 10.1021/jf047878k Devakate, 2009, Purification and drying of bromelain, Sep. Purif. Technol., 64, 259, 10.1016/j.seppur.2008.09.012 Gamboa, 2013, The use of papain inhibitor immobilized onto polyaniline for bioaffinity chromatography of cysteine proteases, Sep. Purif. Technol., 120, 467, 10.1016/j.seppur.2013.10.027 García-Carreño, 1993, Substrate-gel electrophoresis for composition and molecular weight of proteinases of proteinaceous proteinase inhibitor, Anal. Biochem., 214, 65, 10.1006/abio.1993.1457 Goja, 2013, Aqueous two-phase extraction advances for bioseparation, J. Bioprocess. Biotech., 4, 1 Golunski, 2011, Ethanol precipitation and ultrafiltration of inulinases from Kluyveromyces marxianus, Sep. Purif. Technol., 78, 261, 10.1016/j.seppur.2011.02.019 Ha, 2012, Characterisation of commercial papain, bromelain, actinidin and zingibain protease preparations and their activities toward meat proteins, Food Chem., 134, 95, 10.1016/j.foodchem.2012.02.071 Han, 2004, Study on meat tenderness of a protease extracted from domestic pear, Korean J. Food Sci. Anim. Resour., 24, 326 Huddleston, 1991, The molecular basis of partitioning in ATPS: review, TIBTECH, 9, 381, 10.1016/0167-7799(91)90130-A Johansson, 1998, Affinity partitioning of proteins using aqueous two-phase systems, 443 Karnchanatat, 2011, Zingipain, a cysteine protease from Zingiber ottensii valetin rhizomes with antiproliferative activities against fungi and human malignant cell lines, Prep. Biochem. Biotechnol., 41, 138, 10.1080/10826068.2011.547347 Kee, 1998, Application fig protease to foods, Korean J. Food Sci. Anim. Resour., 18, 19 Ketnawa, 2011, Aqueous two-phase extraction of bromelain from pineapple peels (‘Phu Lae’ cultv.) and its biochemical properties, Food Sci. Biotechnol., 20, 1219, 10.1007/s10068-011-0168-5 Kim, 2003, Effects of singular manner or mixed type treatment of proteases isolated from pear, pineapple and kiwifruit on actomyosin degradation, Korean J. Food Sci. Anim. Resour., 23, 193 Kim, 2010, Proteolytic effect of fruit flesh and crude enzyme extract from fruits on myofibrilar protein, Korean J. Food Cookery Sci., 26, 323 Kumar, 1999, Microbial alkaline proteases: from a bio-industrial viewpoint, Biotechnol. Adv., 17, 561, 10.1016/S0734-9750(99)00027-0 Lee, 2001, Encapsulation of bromelain in liposome, J. Food Sci. Nutr., 5, 81 Li, 2009, Purification and characterization of a protease from Korean pear (Pyrus serotine L.) as meat tenderizer, Korean J. Food Sci. Anim. Resour., 29, 157, 10.5851/kosfa.2009.29.2.157 Nam, 2006, The enzymatic properties of actinidin from Kiwifruit, Food Sci. Biotechnol., 15, 453 Nam S.H., Walsh M.K., Kim S.H., Yang K.Y., Identification and functional characterization of cysteine protease from nine pear cultivars (Pyrus pyrifolia), Int. J. Food Prop. in press Naveena, 2004, Tenderizaiton of buffalo meat using plant proteases from Cucumis trigounus Roxb (Kachri) and Zingiber officinale roscoe (Ginger rhizome), Meat Sci., 68, 363, 10.1016/j.meatsci.2004.04.004 Nitsawang, 2006, Purification of papain from Carica papaya latex: aqueous two-phase extraction versus two-step salt precipitation, Enzyme Microbiol. Technol., 39, 1103, 10.1016/j.enzmictec.2006.02.013 Ojha, 1996, Purification, properties and developmental regulation of a Ca2+ independent serine–cysteine protease from Allomyces arbuscular, Int. J. Biochem. Cell Biol., 28, 345, 10.1016/1357-2725(95)00136-0 Pastorello, 1998, Identification of actinidin as the major allergen of kiwi fruit, J. Allergy Clin. Immunol., 101, 531, 10.1016/S0091-6749(98)70360-4 Raghavarao, 1998, Recent developments in aqueous two phase extraction in bioprocessing, Sep. Purif. Method, 27, 1, 10.1080/03602549809351638 Rao, 2011, Novel approach towards recovery of glycosaminoglycans from tannery waste water, Bioresour. Technol., 102, 872, 10.1016/j.biortech.2010.09.013 Rawdkuen, 2011, The partitioning of protease from Calotropis procera latex by aqueous two-phase systems and its hydrolytic pattern on muscle proteins, Food Bioprod. Process., 89, 73, 10.1016/j.fbp.2010.02.001 Rho, 2002, The effect of bulking agent on quality of kiwifruit powder in the process of domestic kiwifruit tenderizer, Korean J. Food Sci., 34, 805 Shyu, 2004, Molecular cloning, expression, and functional characterization of a cystatin from pineapple stem, Biosci. Biotechnol. Biochem., 68, 1681, 10.1271/bbb.68.1681 Simova-Stoilova, 2010, Proteolytic activity and cysteine protease expression in wheat leaves under severe soil drought and recovery, Plant Physiol. Biochem., 48, 200, 10.1016/j.plaphy.2009.11.003 Soares, 2012, Purification of bromelain from pineapple wastes by ethanol precipitation, Sep. Purif. Technol., 98, 389, 10.1016/j.seppur.2012.06.042 Sugiyama, 1996, Purification and characterization of six kiwifruit proteases isolated with two ion-exchange resins, Toyopearl-SuperQ and Bakerbond WP-PEI, Biosci. Biotech. Biochem., 60, 1994, 10.1271/bbb.60.1994 Sun, 2010, Characterization of three novel alkaline serine proteases from tomato (Lycopersicum esculentum mill.) fruit and their potential application, J. Food Biochem., 34, 1014, 10.1111/j.1745-4514.2010.00346.x Zare, 2013, Purification and autolysis of the ficin isoforms from fig (Ficus carica cv. Sabz) latex, Phytochemistry, 87, 16, 10.1016/j.phytochem.2012.12.006