Comparison of N-glycan pattern of recombinant human coagulation factors II and IX expressed in Chinese hamster ovary (CHO) and African green monkey (Vero) cells
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Kurachi K. Recombinant antihemophilic factors. In: Goldstein J, ed. Biotechnology of Blood. Boston: Butterworth-Heinemann, 1992:177–195.
Cumming DA. Physiological relevance of protein glycosylation. Dev Biol Stand 1992;76:83–94.
Rademacher TW. Glycosylation as a factor affecting product consistency. Biologicals 1993;21:103–104.
Werner RG, Noe W. Mammalian cell cultures. Genetic engineering, protein glycosylation, fermentation and process control. Drug Res 1993;43:1242–1249.
Yan SCB, Grinnell B, Wold F. Post-translational modifications of proteins. Trends Biol Sci 1989;14:264–268.
Fischer B, Mitterer A, Dorner F. Purification of recombinant human coagulation factors II and IX and protein S expressed in recombinant Vaccinia virus-infected Vero cells. J Biotechnol 1995;38:129–136.
Bradford M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248–254.
Laemmli UK. Cleavage o structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227:680–685.
Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. Proc Natl Acad Sci USA 1979;76:4350–4354.