Comparison of Microbiological, Histological, and Immunomodulatory Parameters in Response to Treatment with Either Combination Therapy with Prednisone and Metronidazole or Probiotic VSL#3 Strains in Dogs with Idiopathic Inflammatory Bowel Disease

PLoS ONE - Tập 9 Số 4 - Trang e94699
Giacomo Rossi1, G. Pengo2, Marco Caldín3, Angela Palumbo Piccionello1, Jörg M. Steiner4, Noah D. Cohen5, Albert E. Jergens6, Jan S. Suchodolski4
1School of Veterinary Medical Sciences, University of Camerino, Camerino, Italy
2Clinic St. Antonio, Cremona, Italy.
3San Marco Laboratories, Padova, Italy.
4Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, Texas, United States of America
5Department of Large Animal Clinical Sciences, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, Texas, United States of America
6Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, Ames, Iowa, United States of America

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JS Suchodolski, 2011, Companion animals symposium: Microbes and gastrointestinal health of dogs and cats, J Anim Sci, 89, 1520, 10.2527/jas.2010-3377

K Allenspach, 2010, Evaluation of mucosal bacteria and histopathology, clinical disease activity and expression of Toll-like receptors in German shepherd dogs with chronic enteropathies, Vet Microbiol, 146, 326, 10.1016/j.vetmic.2010.05.025

KP Rioux, 2005, The role of enteric microflora in inflammatory bowel disease: Human and animal studies with probiotics and prebiotics, Gastroenterol Clin North Am, 34, 465, 10.1016/j.gtc.2005.05.005

NV Contractor, 1998, Lymphoid hyperplasia, autoimmunity, and compromised intestinal intraepithelial lymphocyte development in colitis-free gnotobiotic IL-2-deficient mice, J Immunol, 160, 385, 10.4049/jimmunol.160.1.385

RK Sellon, 1998, Resident enteric bacteria are necessary for development of spontaneous colitis and immune system activation in interleukin-10-deficient mice, Infect Immun, 66, 5224, 10.1128/IAI.66.11.5224-5231.1998

PG Xenoulis, 2008, Molecular-phylogenetic characterization of microbial communities imbalances in the small intestine of dogs with inflammatory bowel disease, FEMS Microbiol Ecol, 66, 579, 10.1111/j.1574-6941.2008.00556.x

JS Suchodolski, 2010, Molecular analysis of the bacterial microbiota in duodenal biopsies from dogs with idiopathic inflammatory bowel disease, Vet Microbiol, 142, 394, 10.1016/j.vetmic.2009.11.002

JS Suchodolski, 2012, 16S rRNA Gene Pyrosequencing Reveals Bacterial Dysbiosis in the Duodenum of Dogs with Idiopathic Inflammatory Bowel Disease, Plos ONE, 7, e39333, 10.1371/journal.pone.0039333

JS Suchodolski, 2012, The fecal microbiome in dogs with acute diarrhea and idiopathic inflammatory bowel disease, Plos ONE, 7, e51907, 10.1371/journal.pone.0051907

L Sutherland, 1991, Double-Blind, Placebo Controlled Trial of Metronidazole in Crohns-Disease, Gut, 32, 1071, 10.1136/gut.32.9.1071

AE Jergens, 2010, Comparison of oral prednisone and prednisone combined with metronidazole for induction therapy of canine inflammatory bowel disease: a randomized-controlled trial, J Vet Intern Med, 24, 269, 10.1111/j.1939-1676.2009.0447.x

D Turner, 2012, Management of pediatric ulcerative colitis: joint ECCO and ESPGHAN evidence-based consensus guidelines, J Pediatr Gastroenterol Nutr, 55, 340, 10.1097/MPG.0b013e3182662233

SN Sauter, 2006, Effects of probiotic bacteria in dogs with food responsive diarrhoea treated with an elimination diet, J Anim Physiol Anim Nutr (Berl), 90, 269, 10.1111/j.1439-0396.2005.00595.x

R Bibiloni, 2005, VSL#3 probiotic-mixture induces remission in patients with active ulcerative colitis, Am J Gastroenterol, 100, 1539, 10.1111/j.1572-0241.2005.41794.x

Tursi A, Brandimarte G, Papa A, Giglio A, Elisei W, <etal>et al</etal>.. (2010) Treatment of Relapsing Mild-to-Moderate Ulcerative Colitis With the Probiotic VSL#3 as Adjunctive to a Standard Pharmaceutical Treatment: A Double-Blind, Randomized, Placebo-Controlled Study. Am J Gastroenterol: 2218–2227.

KW Simpson, 2011, Pitfalls and progress in the diagnosis and management of canine inflammatory bowel disease, Vet Clin North Am Small Anim Pract, 41, 381, 10.1016/j.cvsm.2011.02.003

AE Jergens, 2003, A scoring index for disease activity in canine inflammatory bowel disease, J Vet Intern Med, 17, 291, 10.1111/j.1939-1676.2003.tb02450.x

AJ German, 2000, Cytokine mRNA expression in mucosal biopsies from German Shepherd dogs with small intestinal enteropathies, Dig Dis Sci, 45, 7, 10.1023/A:1005436721798

Day MJ, Bilzer T, Mansell J, Wilcock B, Hall EJ, <etal>et al</etal>.. (2008) Histopathological Standards for the Diagnosis of Gastrointestinal Inflammation in Endoscopic Biopsy Samples from the Dog and Cat: A Report from the World Small Animal Veterinary Association Gastrointestinal Standardization Group. J Comp Pathol <volume>138</volume>: S1–S43.

G Rossi, 2000, Immunohistochemical study of lymphocyte populations infiltrating the gastric mucosa of beagle dogs experimentally infected with Helicobacter pylori, Infect Immun, 68, 4769, 10.1128/IAI.68.8.4769-4772.2000

AE Ridyard, 2007, Apical junction complex protein expression in the canine colon: differential expression of claudin-2 in the colonic mucosa in dogs with idiopathic colitis, J Histochem Cytochem, 55, 1049, 10.1369/jhc.7A7211.2007

AG Engel, 1986, Mononuclear cells in myopathies: quantitation of functionally distinct subsets, recognition of antigen-specific cell-mediated cytotoxicity in some diseases, and implications for the pathogenesis of the different inflammatory myopathies, Hum Pathol, 17, 704, 10.1016/S0046-8177(86)80180-0

JF Garcia-Mazcorro, 2011, Effect of a multi-species synbiotic formulation on fecal bacterial microbiota of healthy cats and dogs as evaluated by pyrosequencing, FEMS Microbiol Ecol, 78, 542, 10.1111/j.1574-6941.2011.01185.x

D Franchimont, 2004, Deficient host-bacteria interactions in inflammatory bowel disease? The toll-like receptor (TLR)-4 Asp299gly polymorphism is associated with Crohn&apos;s disease and ulcerative colitis, Gut, 53, 987, 10.1136/gut.2003.030205

C Becker, 2006, Cutting edge: IL-23 cross-regulates IL-12 production in T cell-dependent experimental colitis, J Immunol, 177, 2760, 10.4049/jimmunol.177.5.2760

IJ Fuss, 2006, Both IL-12p70 and IL-23 are synthesized during active Crohn&apos;s disease and are down-regulated by treatment with anti-IL-12 p40 monoclonal antibody, Inflamm Bowel Dis, 12, 9, 10.1097/01.MIB.0000194183.92671.b6

H Sokol, 2008, Faecalibacterium prausnitzii is an anti-inflammatory commensal bacterium identified by gut microbiota analysis of Crohn disease patients, PNAS, 105, 16731, 10.1073/pnas.0804812105

B Vitali, 2010, Impact of a synbiotic food on the gut microbial ecology and metabolic profiles, BMC Microbiol, 10, 4, 10.1186/1471-2180-10-4

N Larsen, 2011, Predominant genera of fecal microbiota in children with atopic dermatitis are not altered by intake of probiotic bacteria Lactobacillus acidophilus NCFM and Bifidobacterium animalis subsp. lactis Bi-07, FEMS Microbiol Ecol, 75, 482, 10.1111/j.1574-6941.2010.01024.x

JF Garcia-Mazcorro, 2012, Abundance and short-term temporal variability of fecal microbiota in healthy dogs, MicrobiologyOpen, 1, 340, 10.1002/mbo3.36

VL Wilke, 2012, Gene expression in intestinal mucosal biopsy specimens obtained from dogs with chronic enteropathy, Am J Vet Res, 73, 1219, 10.2460/ajvr.73.8.1219

K Madsen, 2001, Probiotic bacteria enhance murine and human intestinal epithelial barrier function, Gastroenterol, 121, 580, 10.1053/gast.2001.27224

S Prasad, 2005, Inflammatory processes have differential effects on claudins 2, 3 and 4 in colonic epithelial cells, Lab Invest, 85, 1139, 10.1038/labinvest.3700316

N Gassler, 2001, Inflammatory bowel disease is associated with changes of enterocytic junctions, Am J Physiol Gastrointest Liver Physiol, 281, G216, 10.1152/ajpgi.2001.281.1.G216

H Ohta, 2011, Expression and subcellular localization of apical junction proteins in canine duodenal and colonic mucosa, Am J Vet Res, 72, 1046, 10.2460/ajvr.72.8.1046

E Curis, 2005, Almost all about citrullin in mammals, Amino Acids, 29, 177, 10.1007/s00726-005-0235-4

O Dossin, 2011, Effect of Parvoviral Enteritis on Plasma Citrulline Concentration in Dogs, J Vet Intern Med, 25, 215, 10.1111/j.1939-1676.2010.0671.x

D Pinheiro, 2011, Phenotypic and functional characterization of a CD4(+) CD25(high) FOXP3(high) regulatory T-cell population in the dog, Immunol, 132, 111, 10.1111/j.1365-2567.2010.03346.x

CM Colitz, 2000, Histologic and immunohistochemical characterization of lens capsular plaques in dogs with cataracts, Am J Vet Res, 61, 139, 10.2460/ajvr.2000.61.139

J Bassaganya-Riera, 2012, Probiotic bacteria produce conjugated linoleic acid locally in the gut that targets macrophage PPAR gamma to suppress colitis, Plos ONE, 7, e31238, 10.1371/journal.pone.0031238

EO Petrof, 2004, Probiotics inhibit nuclear factor-kappaB and induce heat shock proteins in colonic epithelial cells through proteasome inhibition, Gastroenterol, 127, 1474, 10.1053/j.gastro.2004.09.001

C Di Giacinto, 2005, Probiotics ameliorate recurrent Th1-mediated murine colitis by inducing IL-10 and IL-10-dependent TGF-beta-bearing regulatory cells, J Immunol, 174, 3237, 10.4049/jimmunol.174.6.3237

H Ohta, 2013, Expression of CD4+ T cell cytokine genes in the colorectal mucosa of inflammatory colorectal polyps in miniature dachshunds, Vet Immunol Immunopathol, 155, 259, 10.1016/j.vetimm.2013.07.006

Y Tamura, 2013, Markedly increased expression of interleukin-8 in the colorectal mucosa of inflammatory colorectal polyps in miniature dachshunds, Vet Immunol Immunopathol, 156, 32, 10.1016/j.vetimm.2013.09.017

M Muhling, 2008, Improved group-specific PCR primers for denaturing gradient gel electrophoresis analysis of the genetic diversity of complex microbial communities, ISME J, 2, 379, 10.1038/ismej.2007.97

E Malinen, 2005, Analysis of the fecal microbiota of irritable bowel syndrome patients and healthy controls with real-time PCR, Am J Gastroenterol, 100, 373, 10.1111/j.1572-0241.2005.40312.x

JF Garcia-Mazcorro, 2012, Effect of the proton pump inhibitor omeprazole on the gastrointestinal bacterial microbiota of healthy dogs, FEMS Microbiol Ecol, 80, 624, 10.1111/j.1574-6941.2012.01331.x

MG Wise, 2005, Quantitative detection of Clostridium perfringens in the broiler fowl gastrointestinal tract by real-time PCR, Appl Environ Microbiol, 71, 3911, 10.1128/AEM.71.7.3911-3916.2005

DC Lubbs, 2009, Dietary protein concentration affects intestinal microbiota of adult cats: a study using DGGE and qPCR to evaluate differences in microbial populations in the feline gastrointestinal tract, J Anim Physiol Anim Nutr (Berl), 93, 113, 10.1111/j.1439-0396.2007.00788.x

T Rinttila, 2004, Development of an extensive set of 16S rDNA-targeted primers for quantification of pathogenic and indigenous bacteria in faecal samples by real-time PCR, J Appl Microbiol, 97, 1166, 10.1111/j.1365-2672.2004.02409.x

JP Furet, 2004, Molecular quantification of lactic acid bacteria in fermented milk products using real-time quantitative PCR, Int J Food Microbiol, 97, 197, 10.1016/j.ijfoodmicro.2004.04.020

J Walter, 2001, Detection of Lactobacillus, Pediococcus, Leuconostoc, and Weissella species in human feces by using group-specific PCR primers and denaturing gradient gel electrophoresis, Appl Environ Microbiol, 67, 2578, 10.1128/AEM.67.6.2578-2585.2001

HG Heilig, 2002, Molecular diversity of Lactobacillus spp. and other lactic acid bacteria in the human intestine as determined by specific amplification of 16S ribosomal DNA, Appl Environ Microbiol, 68, 114, 10.1128/AEM.68.1.114-123.2002