Comparison of LPS and MS-induced depressive mouse model: behavior, inflammation and biochemical changes
Tóm tắt
Depression is a mental disease involving complex pathophysiological mechanisms, and there are many ways to establish depressive mouse models. The purpose of this study is to comprehensively compare the behavioral changes and its mechanism induced by two different models. This study established two depressive mouse models by maternal separation (MS) or lipopolysaccharide (LPS) administration, and added fluoxetine treatment group respectively for comparison. MS induced more apparent anxiety-like behavior while LPS induced more apparent depressive-like behavior. LPS increased peripheral inflammatory factors more apparent, which were mitigated by fluoxetine. MS inhibited the 5-HT system more obviously and was relieved by fluoxetine. LPS triggered stronger immune response in the hippocampus and prefrontal cortex (PFC). MS significantly reduced the expression of neurotrophic proteins and was alleviated by fluoxetine. Overall, LPS induced stronger system inflammation, while MS impaired the function of HPA axis and 5-HT system. Our results will contribute to a deeper understanding of the pathophysiology of different stress-induced depression and will also help researchers select appropriate models of depression for their own needs.
Tài liệu tham khảo
Malhi GS, Mann JJ. Depression. Lancet. 2018;392(10161):2299–312. https://doi.org/10.1016/S0140-6736(18)31948-2.
WHO: Depression.|.*2021*2021.: World Health Organization; 2021.
Iob E, Kirschbaum C, Steptoe A. Persistent depressive symptoms, HPA-axis hyperactivity, and inflammation: the role of cognitive-affective and somatic symptoms. Mol Psychiatry. 2020;25(5):1130–40. https://doi.org/10.1038/s41380-019-0501-6.
Price RB, Duman R. Neuroplasticity in cognitive and psychological mechanisms of depression: an integrative model. Mol Psychiatry. 2020;25(3):530–43. https://doi.org/10.1038/s41380-019-0615-x.
Beurel E, Toups M, Nemeroff CB. The bidirectional relationship of depression and inflammation: double trouble. Neuron. 2020;107(2):234–56. https://doi.org/10.1016/j.neuron.2020.06.002.
Penner-Goeke S, Binder EB. Epigenetics and depression. Dialogues Clin Neurosci. 2019;21(4):397–405 https://doi.org/10.31887/DCNS.2019.21.4/ebinder.
Nemeroff CB. Paradise lost: the neurobiological and clinical consequences of child abuse and neglect. Neuron. 2016;89(5):892–909. https://doi.org/10.1016/j.neuron.2016.01.019.
Suchecki D. Maternal regulation of the infant's hypothalamic-pituitary-adrenal axis stress response: Seymour 'Gig' Levine's legacy to neuroendocrinology. J Neuroendocrinol. 2018;30(7):e12610. https://doi.org/10.1111/jne.12610.
van Bodegom M, Homberg JR, Henckens M. Modulation of the hypothalamic-pituitary-adrenal Axis by early life stress exposure. Front Cell Neurosci. 2017;11:87. https://doi.org/10.3389/fncel.2017.00087.
Nishi M. Effects of early-life stress on the brain and behaviors: implications of early maternal separation in rodents. Int J Mol Sci. 2020;21(19). https://doi.org/10.3390/ijms21197212.
Miyazaki T, Takase K, Nakajima W, Tada H, Ohya D, Sano A, et al. Disrupted cortical function underlies behavior dysfunction due to social isolation. J Clin Invest. 2012;122(7):2690–701. https://doi.org/10.1172/JCI63060.
Zajdel J, Zager A, Blomqvist A, Engblom D, Shionoya K. Acute maternal separation potentiates the gene expression and corticosterone response induced by inflammation. Brain Behav Immun. 2019;77:141–9. https://doi.org/10.1016/j.bbi.2018.12.016.
Ho PS, Yen CH, Chen CY, Huang SY, Liang CS. Changes in cytokine and chemokine expression distinguish dysthymic disorder from major depression and healthy controls. Psychiatry Res. 2017;248:20–7. https://doi.org/10.1016/j.psychres.2016.12.014.
Goldsmith DR, Rapaport MH, Miller BJ. A meta-analysis of blood cytokine network alterations in psychiatric patients: comparisons between schizophrenia, bipolar disorder and depression. Mol Psychiatry. 2016;21(12):1696–709. https://doi.org/10.1038/mp.2016.3.
Eyre HA, Air T, Pradhan A, Johnston J, Lavretsky H, Stuart MJ, et al. A meta-analysis of chemokines in major depression. Prog Neuro-Psychopharmacol Biol Psychiatry. 2016;68:1–8. https://doi.org/10.1016/j.pnpbp.2016.02.006.
Black C, Miller BJ. Meta-analysis of cytokines and chemokines in Suicidality: distinguishing suicidal versus nonsuicidal patients. Biol Psychiatry. 2015;78(1):28–37. https://doi.org/10.1016/j.biopsych.2014.10.014.
Li W, Ali T, He K, Liu Z, Shah FA, Ren Q, et al. Ibrutinib alleviates LPS-induced neuroinflammation and synaptic defects in a mouse model of depression. Brain Behav Immun. 2021;92:10–24. https://doi.org/10.1016/j.bbi.2020.11.008.
Li M, Li C, Yu H, Cai X, Shen X, Sun X, et al. Lentivirus-mediated interleukin-1beta (IL-1beta) knock-down in the hippocampus alleviates lipopolysaccharide (LPS)-induced memory deficits and anxiety- and depression-like behaviors in mice. J Neuroinflammation. 2017;14(1):190. https://doi.org/10.1186/s12974-017-0964-9.
Muhammad T, Ikram M, Ullah R, Rehman SU, Kim MO. Hesperetin, a Citrus flavonoid, attenuates LPS-induced Neuroinflammation, apoptosis and memory impairments by modulating TLR4/NF-kappaB signaling. NUTRIENTS. 2019;11(3). https://doi.org/10.3390/nu11030648.
Arioz BI, Tastan B, Tarakcioglu E, Tufekci KU, Olcum M, Ersoy N, et al. Melatonin attenuates LPS-induced acute depressive-like behaviors and microglial NLRP3 Inflammasome activation through the SIRT1/Nrf2 pathway. Front Immunol. 2019;10:1511. https://doi.org/10.3389/fimmu.2019.01511.
Wong DT, Perry KW, Bymaster FP. Case history: the discovery of fluoxetine hydrochloride (Prozac). Nat Rev Drug Discov. 2005;4(9):764–74. https://doi.org/10.1038/nrd1821.
George ED, Bordner KA, Elwafi HM, Simen AA. Maternal separation with early weaning: a novel mouse model of early life neglect. BMC Neurosci. 2010;11:123. https://doi.org/10.1186/1471-2202-11-123.
Wang X, Yu H, Wang C, Liu Y, You J, Wang P, et al. Chronic ethanol exposure induces neuroinflammation in H4 cells through TLR3 / NF-kappaB pathway and anxiety-like behavior in male C57BL/6 mice. Toxicology. 2020;446:152625. https://doi.org/10.1016/j.tox.2020.152625.
Wen G, Yao H, Li Y, Ding R, Ren X, Tan Y, et al. Regulation of tau protein on the antidepressant effects of ketamine in the chronic unpredictable mild stress model. Front Psychiatr. 2019;10:287. https://doi.org/10.3389/fpsyt.2019.00287.
Yao H, Shen H, Yu H, Wang C, Ding R, Lan X, et al. Chronic ethanol exposure induced depressive-like behavior in male C57BL/6 N mice by downregulating GluA1. Physiol Behav. 2021;234:113387. https://doi.org/10.1016/j.physbeh.2021.113387.
Yao H, Guo W, Suo L, Li G, Wang Y, Chen Y, et al. Preventive effects of the AMPA receptor potentiator LY450108 in an LPS-induced depressive mouse model. Behav Brain Res. 2022;424:113813. https://doi.org/10.1016/j.bbr.2022.113813.
Yao H, Zhang D, Yu H, Shen H, Lan X, Liu H, et al. Chronic ethanol exposure induced anxiety-like behavior by altering gut microbiota and GABA system. Addict Biol. 2022;27(5):e13203. https://doi.org/10.1111/adb.13203.
Yao H, Zhang D, Yu H, Shen H, Lan X, Liu H, et al. AMPAkine CX516 alleviated chronic ethanol exposure-induced neurodegeneration and depressive-like behavior in mice. Toxicol Appl Pharmacol. 2022;439:115924. https://doi.org/10.1016/j.taap.2022.115924.
Binley KE, Ng WS, Tribble JR, Song B, Morgan JE. Sholl analysis: a quantitative comparison of semi-automated methods. J Neurosci Methods. 2014;225:65–70. https://doi.org/10.1016/j.jneumeth.2014.01.017.
Lai JL, Liu YH, Liu C, Qi MP, Liu RN, Zhu XF, et al. Indirubin inhibits LPS-induced inflammation via TLR4 abrogation mediated by the NF-kB and MAPK signaling pathways. Inflammation. 2017;40(1):1–12. https://doi.org/10.1007/s10753-016-0447-7.
Corona AW, Norden DM, Skendelas JP, Huang Y, O'Connor JC, Lawson M, et al. Indoleamine 2,3-dioxygenase inhibition attenuates lipopolysaccharide induced persistent microglial activation and depressive-like complications in fractalkine receptor (CX(3)CR1)-deficient mice. Brain Behav Immun. 2013;31:134–42. https://doi.org/10.1016/j.bbi.2012.08.008.
Aisa B, Tordera R, Lasheras B, Del RJ, Ramirez MJ. Effects of maternal separation on hypothalamic-pituitary-adrenal responses, cognition and vulnerability to stress in adult female rats. Neuroscience. 2008;154(4):1218–26. https://doi.org/10.1016/j.neuroscience.2008.05.011.
Holmes A, le Guisquet AM, Vogel E, Millstein RA, Leman S, Belzung C. Early life genetic, epigenetic and environmental factors shaping emotionality in rodents. Neurosci Biobehav Rev. 2005;29(8):1335–46. https://doi.org/10.1016/j.neubiorev.2005.04.012.
Frankiensztajn LM, Elliott E, Koren O. The microbiota and the hypothalamus-pituitary-adrenocortical (HPA) axis, implications for anxiety and stress disorders. Curr Opin Neurobiol. 2020;62:76–82. https://doi.org/10.1016/j.conb.2019.12.003.
Jones KA, Srivastava DP, Allen JA, Strachan RT, Roth BL, Penzes P. Rapid modulation of spine morphology by the 5-HT2A serotonin receptor through kalirin-7 signaling. Proc Natl Acad Sci U S A. 2009;106(46):19575–80. https://doi.org/10.1073/pnas.0905884106.
Kraus C, Castren E, Kasper S, Lanzenberger R. Serotonin and neuroplasticity - links between molecular, functional and structural pathophysiology in depression. Neurosci Biobehav Rev. 2017;77:317–26. https://doi.org/10.1016/j.neubiorev.2017.03.007.