Comparison of BALB/c and B6-albino mouse strain blastocysts as hosts for the injection of C57BL6/N-derived C2 embryonic stem cells
Tóm tắt
Embryonic stem (ES) cells from a C57BL/6N (B6N) background injected into B6(Cg)-Tyrc-2J/J (B6-albino) recipient blastocysts are commonly used for generating genetically modified mouse models. To understand the influence of the recipient blastocyst strain on germline transmission, BALB/cAnNTac and B6-albino germline transmission rates were compared using the C57BL6/N-derived C2 ES cell line. A total of 92 ES cell clones from 27 constructs were injected. We compared blastocyst yield, birth rate, chimera formation rate, and high-percentage (>50 %) male chimera formation rate. For germline transmission, we analyzed 24 clones from 19 constructs, which generated high-percentage male chimeras from both donor strains. B6-albino hosts resulted in higher mean blastocyst yields per donor than did BALB/c ones (3.6 vs. 2.5). However, BALB/c hosts resulted in a higher birth rate than B6-albino ones (36 vs. 27 %), a higher chimera formation rate (50 vs. 42 %), a higher high-percentage male chimera rate (10 vs. 8 %), and a higher germline transmission rate (65 vs. 49 %), respectively. Our data suggest that BALB/c is a suitable blastocyst host strain for C2 ES cells and has an advantage over the B6-albino strain for receiving the injection of C2 ES cells.
Tài liệu tham khảo
Auerbach W, Dunmore JH, Fairchild-Huntress V, Fang Q, Auerbach AB, Huszar D, Joyner AL (2000) Establishment and chimera analysis of 129/SvEv- and C57BL/6-derived mouse embryonic stem cell lines. Biotechniques 29(5):1024–1028, 1030, 1032
Behringer R, Gertsenstein M, Nagy K, Nagy A (2013) Manipulating the mouse embryo: a laboratory manual, 4th edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor
Dolgin E (2011) Mouse library set to be knockout. Nature 474(7351):262–263
Fielder TJ, Yi CS, Masumi J, Waymire KG, Chen HW, Wang S, Shi KX, Wallace DC, MacGregor GR (2012) Comparison of male chimeric mice generated from microinjection of JM8.N4 embryonic stem cells into C57BL/6J and C57BL/6NTac blastocysts. Transgenic Res 21(6):1149–1158
Gertsenstein M, Nuter LM, Reid T, Pereira M, Stanford WL, Rossant J, Nagy A (2010) Efficient generation of germ line transmitting chimeras from C57BL/6N ES cells by aggregation with outbred host embryos. PLoS ONE 5(6):e11260. doi:10.1371/journal.pone.0011260
Hansen GM, Markesich DC, Burnett MB, Zhu Q, Dionne KM, Richter LJ, Finnell RH, Sands AT, Zambrowicz BP, Abuin A (2008) Large-scale gene trapping in C57BL/6N mouse embryonic stem cells. Genome Res 18(10):1670–1679
Ledermann B, Burki K (1991) Establishment of germ-line competent C57BL/6 embryonic stem cell line. Exp Cell Res 197(2):254–258
Lemckert FA, Sedgwick JD, Korner H (1997) Gene targeting in C57BL/6 ES cells. Successful germ line transmission using recipient BALB/c blastocysts developmentally matured in vitro. Nucleic Acids Res 25(4):917–918
Pacholczyk G, Suhag R, Mazurek M, Dederscheck SM, Koni PA (2008) Generation of C57BL/6 knockout mice using C3HxBALB/c blastocysts. Biotechniques 44(3):413–416
Papaioannou V, Johnson R (2000) Production of chimeras by blastocyst and morula injection of targeted ES cells. In: Joyner AL (ed) Gene targeting: a practical approach, 2nd edn. Oxford University Press, Oxford, pp 133–175
Pettitt SJ, Liang Q, Rairdan XY, Moran JL, Prosser HM, Beier DR, Lloyd KC, Bradley A, Skarnes WC (2009) Agouti C57BL/6N embryonic stem cells for mouse genetic resources. Nat Methods 6(7):493–496
Poueymirou WT, Auerbach W, Frendewey D, Hickey JF, Escaravage JM, Esau L, Doré AT, Stevens S, Adams NC, Dominguez MG, Gale NW, Yancopoulos GD, DeChiara TM, Valenzuela DM (2007) F0 generation mice fully derived from gene-targeted embryonic stem cells allowing immediate phenotypic analyses. Nat Biotechnol 25(1):91–99
Schuster-Gossler K, Lee AW, Lerner CP, Parker HJ, Dyer VW, Scott VE, Gossler A, Conover JC (2001) Use of coisogenic host blastocysts for efficient establishment of germline chimeras with C57BL/6J ES cell lines. Biotechniques 31(5):1022–1024, 1026
Seong E, Saunders TL, Stewart CL, Burmeister M (2004) To knockout in 129 or in C57BL/6: that is the question. Trends Genet 20(2):59–62
Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (2011) A conditional knockout resource for the genome-wide study of mouse gene function. Nature 474(7351):337–342
Taft RA, Low BE, Byers SL, Murray SA, Kutny P, Wiles MV (2013) The Perfect Host: a mouse host embryo facilitating more efficient germ line transmission of genetically modified embryonic stem cells. PLoS ONE 8(7):e67826. doi:10.1371/journal.pone.0067826
Zevnik B, Uyttersprot NC, Perez AV, Bothe GW, Kern H, Kauselmann G (2014) C57BL/6N albino/agouti mutant mice as embryo donors for efficient germline transmission of C57BL/6 ES cells. PLoS ONE 9(3):e90570. doi:10.1371/journal.pone.0090570