Comparison of 5 microplate colorimetric assays forin vitro cytotoxicity testing and cell proliferation assays

Alley MC, Scudiero DA, Monks A, Hursey ML, Czerwinsk MJ, Fine DL, Abbott BJ, Mayo JG, Shoemaker RH and Boyd MR (1988) Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay. Cancer Res. 48: 589?601.

Borenfreund E and Puemer JA (1983) A simple quantitative procedure using monolayer cultures for cytotoxicity assays. J. Tissue Culture Meth. 9: 7?9.

Connolly DT, Knight MB, Harakas NK, Wittwer AJ and Feder J (1986) Determination of the number of endothelial cells in culture versus an acid phosphatase assay. Anal. Biochem. 152: 136?140.

Cook JA and Mitchell JB (1989) Viability measurements in mammalian cell systems. Anal. Biochem. 179: 1?7.

Fiennes A, Walton J, Winterboume D, McGlashan D and Hermon-Taylor J (1987) Quantitative correlation of natural red dye uptake with cell numbers in human cancer cell cultures. Cell Biol. Int. Repts. 11: 373?378.

Gilvarry U, Farrell D, Lynch V, Moriarty M, Dooley M, Law E and Clynes M (1990) Cytological differences between normal and malignant cell populations in culture. Cancer Res. 50, 3390?3393.

Givens K, Kirada S, Chen A, Rothschiller J and Lee D (1990) Purification of human ocular fibroblasts. An assessment ofin vitro colorimetric assays. Invest. Ophthalmol. Vis. Sci. 31: 1856?1862.

Huschtscha LI, Lucibello FC and Bodmer WF (1989) A rapid method for counting cells ?in situ? using a fluorogenic alkaline phosphatase enzyme assay. In Vitro Cell Dev. Biol. 25: 105?108.

Keepers YP, Pizao FE, Peters GJ, Vanarkotte J, Winograd B and Pinedo HM (1991) Comparison of the sulforhodamine-B protein and tetrazolium (MTT) assays forin vitro chemosensitivity testing. Eur. J. Cancer 27: 897?900.

Kueng W, Silber E and Eppenberger U (1989) Quantification of cells cultured on 96-well plates. Anal. Biochem. 182: 16?19.

Martin A and Clynes M (1991) Acid phosphatase: endpoint forin vitro toxicity tests. In Vitro Cell Dev. Biol. 27A: 183?184.

Miura M, Kunitomo K and Komi N (1988)In vitro chemosensitivity testing based on intracellular ATF levels (ATP assay). Tokushima. J. Exp. Med 35: 49?55.

Mossman T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Meth. 65: 55?63.

Neville ME (1987)51Cr uptake assay: a sensitive and reliable method to quantitate cell viability and cell death. J. Immunol. Meth. 99: 77?82.

Scragg M and Ferreira L (1991) Evaluation of differential staining procedures for quantification of fibroblasts cultured in 96-well plates. Anal. Biochem. 198: 80?85.

Skehan F, Storeng R, Scudiero D, Monks A, McMahon J, Vistico D, Warren J, Bokesch H, Kenney S and Boyd MR (1990) New colorimetric assay of anticancer drug screening. J. Natl. Canc. Inst. 82: 1107?1113.

Triglia D, Braa S, Yonan C and Naughton G (1991)In vitro toxicity of various classes of test agents using the neutral red assay on a human three-dimensional physiologic skin model. In Vitro Cell Dev. Biol. 27A: 239?244.

Vistica DT, Skehan P, Scudiero D, Monks A, Pittman A and Boyd MR (1991) Tetrazolium-based assays for cellular viability: a critical examination of selected parameters affecting formazan production. Cancer Res. 51: 2515?2520.