Comparative of fibroblast and osteoblast cells adhesion on surface modified nanofibrous substrates based on polycaprolactone
Tóm tắt
One of the determinant factors for successful bioengineering is to achieve appropriate nano-topography and three-dimensional substrate. In this research, polycaprolactone (PCL) nano-fibrous mat with different roughness modified with O2 plasma was fabricated via electrospinning. The purpose of this study was to evaluate the effect of plasma modification along with surface nano-topography of mats on the quality of human fibroblast (HDFs) and osteoblast cells (OSTs)-substrate interaction. Surface properties were studied using scanning electron microscopy (SEM), atomic force microscopy (AFM), contact angle, Fourier-transformation infrared spectroscopy. We evaluated mechanical properties of fabricated mats by tensile test. The viability and proliferation of HDFs and OSTs on the substrates were followed by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT). Mineralization of the substrate was determined by alizarin red staining method and calcium content of OSTs was determined by calcium content kit. Cells morphology was studied by SEM analysis. The results revealed that the plasma-treated electrospun nano-fibrous substrate with higher roughness was an excellent designed substrate. A bioactive topography for stimulating proliferation of HDFs and OSTs is to accelerate the latter’s differentiation time. Therefore, the PCL substrate with high density and major nano-topography were considered as a bio-functional and elegant bio-substrate for tissue regeneration applications.
Tài liệu tham khảo
Acevedo CA, Somoza RA, Weinstein-Oppenheimer C et al (2013) Improvement of human skin cell growth by radiation-induced modifications of a Ge/Ch/Ha scaffold. J Bioprocess Biosyst Eng 8:305–313
Adams JC (2002) Methods in cell biology: methods in cell-matrix adhesion. Elsevier, San Diego, p 32
Ambrosio L, Guarino V (2008) The synergic effect of polylactide fiber and calcium phosphate particle reinforcement in poly epsilon-caprolactone-based composite scaffolds. J Acta Biomater 4:1778–1787
Atyabi SM, Irani S, Sharifi F et al (2016) Cell attachment and viability study of PCL nano-fiber modified by cold atmospheric plasma. J Cell Biochem Biophys 74:181–190
Besunder R, Papas D, Yildirim ED et al (2010) Accelerated differentiation of osteoblast cells on polycaprolactone scaffolds driven by a combined effect of protein coating and plasma modification. J Biofabr 2:1–12
Chan CK, Prabhakaran MP, Venugopal J et al (2008) Surface modified electrospun nanofibrous scaffolds for nerve tissue engineering. J Nanotechnol 19:455102
Cheng Y, Lee P, Ramos D et al (2014) Collagen functionalized bioactive nanofiber matrices for osteogenic differentiation of mesenchymal stem cells: bone tissue engineering. J Biomed Nanotechnol 10:287–298
Dehghan M, Homayouni Moghadam F, Tayebi T et al (2014) Differentiation of bone marrow mesenchymal stem cells into chondrocytes after short term culture in alkaline medium. J IJHOSCR 8:13–19
Demirtas TT, Gumusderelioglu M, Mavis B et al (2009) Synthesis, characterization and osteoblastic activity of polycaprolactone nano-fibers coated with biomimetic calcium phosphate. J Acta Biomater 5:3098–3111
Desai S, Singh RP (2004) Surface modification of polyethylene. Long-term properties of polyolefins. Springer, New York, pp 231–294
Dhanasekaran M, Kamath MS, Shiek SSJA et al (2014) Polycaprolactone scaffold engineered for sustained release of resveratrol: therapeutic enhancement in bone. J Int Nanomed 9:183–195
Domingos M, Gloria A, Intranuovo F et al (2013) Improved osteoblast cell affinity on plasma-modified 3-dextruded PCL scaffolds. J Acta Biomater 9:5997–6005
Farooque TM, Kumar G, Waters MS et al (2012) Freeform fabricated scaffolds with roughness struts that enhance both stem cell proliferation and differentiation by controlling cell shape. J Biomater 33:4022–4030
Forch R, Jenkins ATA, Schonherr H (2009) Surface design: applications in bioscience and nanotechnology. Wiley, New York, pp 271–282
Gazicki M, Yasuda H (1982) Biomedical applications of plasma polymerization and plasma treatment of polymer surface. J Biomater 3:68–72
Grace JM, Gerenser LJ (2003) Plasma treatment of polymers. Dispers J Sci Technol 24:305–341
Greene G, Tannenbaum R, Yao G et al (2003) Wetting characteristics of plasma-modified porous polyethylene. J Langmuir 19:5869–5874
Guadalupe E, Ramos D, Shelke NB et al (2015) Bioactive polymeric nanofiber matrices for skin regeneration. J Appl Polym 132:41879
Hourston DJ, Lane JM (1993) Surface treatments of polyolefins. J Prog Org Coat 21:269–284
Hui S, Ji Z, Kuikui H et al (2010) Compressed collagen gel as the scaffold for skin engineering. J Biomed Micro Devices 12:627–635
Irani S, Salamian N, Zandi M et al (2013) Cell attachment studies on electrospun nanofibrous PLGA and freeze-dried porous PLGA. J Nano Bull 2:130103–130107
Irani S, Salamian N, Zandi M et al (2014) The study of P19 stem cell behavior on aligned oriented electrospun poly(lactic-co-glycolic acid) nano-fibers for neural tissue engineering. J Polym Adv Technol 25:562–567
James R, Nukavarapu SP, Kumbar SG et al (2008) Electrospun poly (lactic acid-co-glycolic acid) scaffolds for skin tissue engineering. J Biomater 29:4100–4107
James R, Kumbar SG, Laurencin CT et al (2011) Electrospun nanofibrous scaffolds for engineering soft connective tissues. J Method Mol Biol 726:243–258
Johnson J, Niehaus A, Nichols S et al (2009) Electrospun PCL in vitro: a microstructural basis for mechanical property changes. J Biomater Sci Polym 20:467–481
Kluger PJ, Weisser J, Wyrwa R et al (2010) Electrospun poly(d/l-lactide-co-l-lactide) hybrid matrix: a novel scaffold material for soft tissue engineering. J Mater Sci 21:2665–2671
Knezevic M, Novakovic GV, Marolt D (2010) Bone tissue engineering with human stem cells. J Stem Cell Res Ther 1:10. doi:10.1186/scrt10
Krishnan R, Rajeswari R, Venugopal J et al (2012) Polysaccharide nanofibrous scaffolds as a model for in vitro skin tissue regeneration. J Mater Sci 23:1511–1519
Kumbar SG, James R, Nukavarapu SP et al (2008) Electrospun nanofiber scaffolds: engineering soft tissue. J Biomed Mater. doi:10.1088/1748-6041/3/3/034002
Lee HUK, Sul JY, Young JS et al (2008) Role of reactive gas in atmospheric plasma for cell attachment and proliferation on biocompatible poly caprolactone film. J Appl Surf Sci 254:5700–5705
López-Pérez PM, Silva RMP, Sousa RA et al (2010) Plasma-induced polymerization as a tool for surface functionalization of polymer scaffolds for bone tissue engineering: an in vitro study. J Acta Biomater 6:3704–3712
Mirzadeh H, Saeed M, Zandi M et al (2015) Rationalization of specific structure formation in electrospinning process: study on nano-fibrous PCL- and PLGA-based scaffolds. J Biomed Mater Res Part A 103(12):3927–3939
Moon TS, Shim JH, Yun MJ et al (2012) Stimulation of healing within a rabbit calvarial defect by a PCL/PLGA scaffold blended with TCP using solid freeform fabrication technology. J Mater Sci 23:2993–3002
Swindle-Reilly Katelyn E, Paranjape Chinmay S, Miller Cheryl A (2014) Electrospun poly(caprolactone)-elastin scaffolds for peripheral nerve regeneration. Prog Biomater 3:20