Comparative genome‐wide analysis of small RNAs of major Gram‐positive pathogens: from identification to application

Microbial Biotechnology - Tập 3 Số 6 - Trang 658-676 - 2010
Mobarak Abu Mraheil1,2, André Billion1,2, Carsten Kuenne2, Jordan Pischimarov2, Bernd Kreikemeyer3, Susanne Engelmann4, Axel Hartke5, Jean‐Christophe Giard5, Maja Rupnik6, Sonja Vorwerk7, Markus Beier7, Julia van der Meer8, Thomas Hartsch8, Anette Jacob9, Franz Cemič10, Jürgen Hemberger10, Trinad Chakraborty2, Torsten Hain2
1Both coauthors contributed equally to the work.
2Institute of Medical Microbiology, Justus-Liebig-University, Frankfurter Strasse 107, 35392 Giessen, Germany.
3Institute for Medical Microbiology, University of Rostock, Schillingallee 70, 18057 Rostock, Germany.
4Institute for Microbiology, University of Greifswald, Jahnstrasse 15, 17487 Greifswald, Germany.
5Laboratoire de Microbiologie de l'Université de Caen, EA956 USC INRA2017, 14032 CAEN cedex, France.
6University of Maribor, Faculty of Medicine, Slomskov trg 15, 2000 Maribor, Slovenia.
7febit biomed gmbh, Im Neuenheimer Feld 519, 69120 Heidelberg, Germany
8Genedata Bioinformatik GmbH, Lena-Christ-Str. 50, D-82152 Planegg-Martinsried, Germany.
9Functional Genome Analysis, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
10Institute for Biochemical Engineering and Analytics, University of Applied Sciences Giessen-Friedberg, Wiesenstasse 14, 35390 Giessen, Germany.

Tóm tắt

SummaryIn the recent years, the number of drug‐ and multi‐drug‐resistant microbial strains has increased rapidly. Therefore, the need to identify innovative approaches for development of novel anti‐infectives and new therapeutic targets is of high priority in global health care. The detection of small RNAs (sRNAs) in bacteria has attracted considerable attention as an emerging class of new gene expression regulators. Several experimental technologies to predict sRNA have been established for the Gram‐negative model organism Escherichia coli. In many respects, sRNA screens in this model system have set a blueprint for the global and functional identification of sRNAs for Gram‐positive microbes, but the functional role of sRNAs in colonization and pathogenicity for Listeria monocytogenes, Staphylococcus aureus, Streptococcus pyogenes, Enterococcus faecalis and Clostridium difficile is almost completely unknown. Here, we report the current knowledge about the sRNAs of these socioeconomically relevant Gram‐positive pathogens, overview the state‐of‐the‐art high‐throughput sRNA screening methods and summarize bioinformatics approaches for genome‐wide sRNA identification and target prediction. Finally, we discuss the use of modified peptide nucleic acids (PNAs) as a novel tool to inactivate potential sRNA and their applications in rapid and specific detection of pathogenic bacteria.

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Microbial Biotechnology

Tập 3 Số 6

658-676

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