Comparative evaluation of healing response between colo-colic invagination anastomosis and single-layer running suture. Experimental study in dogs

Journal of Coloproctology - Tập 36 - Trang 157-161 - 2016
Miguel Augusto Arcoverde Nogueira1, Francisco Sérgio Pinheiro Regadas2, Carlos Renato Sales Bezerra1,3,4, Welligton Ribeiro Figueiredo4,5, Erbert Portela Martins Filho5
1Universidade Estadual do Piauí (UESPI), Escola de Medicina, Teresina, PI, Brazil
2Universidade Federal do Ceará (UFC), Escola de Medicina, Fortaleza, CE, Brazil
3Programa de Cirurgia Abdominal, Universidade Estadual do Piauí (UESPI), Teresina, PI, Brazil
4Universidade Federal do Ceará (UFC), Fortaleza, CE, Brazil
5Faculdade Integral Diferencial (FacidDevry), Teresina, PI, Brazil

Tóm tắt

AbstractObjective Evaluate healing response of colo-colic anastomosis by invagination vs. single-layer suture.Methods Sixty dogs were randomly distributed in two groups and anastomosed with single-layer suture (G-I, control) or by invagination and cardinal sutures (G-II, study). In the end, the animals were euthanized (10 from each group on POD7 and 20 on POD21) and the anastomosed segment was retrieved for histology and immunohistochemistry. Parameters included body weight, adhesions, edema, vasoproliferation, type I and III collagen, myeloperoxidase and nitric oxide. Findings were analyzed with Student's t test and the Mann–Whitney test.Results No animal died prior to euthanasia. The groups were similar with regard to all parameters: median weight 10.86 kg (G-I) and 9.98 kg (G-II) on POD7 (p = 0.41) and 11.86 kg (G-I) and 11.55 kg (G-II) on POD21 (p = 0.71); abdominal adhesions (p = 0.7383 POD7; p = 0.5685 POD21), level of edema (p = 0.3006 POD7; p = 0.7990 POD21), vasoproliferation (p = 0.1191 POD7; p = 0.0758 POD21), type I collagen (p = 0.4591 POD7; p = 0.3357 POD21), type III collagen (p = 0.2166 POD7; p = 0.2712 POD21), nitric oxide (p = 0.3980 POD7; p = 0.4796 POD21) and myeloperoxidase (p = 0.580 POD7; p = 0.755 POD21).Conclusion No significant difference in healing response was observed between the two anastomosis techniques (single-layer suture and invagination).

Tài liệu tham khảo

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Journal of Coloproctology

Tập 36

157-161

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