Comparative efficacy of two fipronil spot-on formulations against experimental tick infestations (Ixodes ricinus) in dogs

Parasitology Research - Tập 107 - Trang 735-739 - 2010
Stéphane Bonneau1, Sandeep Gupta2, Marie-Christine Cadiergues3
1Virbac S.A. Preclinical and Clinical Unit, Carros, France
2Charles River Laboratories Preclinical Services Ireland Ltd, Mayo, Ireland
3Dermatology, Groupe de Recherche des Animaux de Compagnie, National Veterinary School, Toulouse, France

Tóm tắt

A parallel-group-design, randomized, unicentre and blinded controlled study was undertaken to assess the efficacy of a new fipronil-based spot-on formulation applied once to dogs against experimental Ixodes ricinus infestations. Six dogs served as negative controls (group 1), six dogs served as positive controls (group 2) receiving the original fipronil spot-on (Frontline® spot-on Dog, Merial) at a dosage of 0.67 mL for a dog weighing from 2 to 10 kg and 1.34 mL for a dog weighing from 10.1 to 20 kg and six dogs were treated with a 10% w/v fipronil-based spot-on solution (Effipro® Spot-on, Virbac SA) at an identical dosage (group 3, 0.67 mL for a dog weighing from 2 to 10 kg and 1.34 mL for a dog weighing from 10.1 to 20 kg). Each dog was sedated and subsequently infested with 50 unfed adult I. ricinus on days −7, −2, 7, 14, 21, 28 and 35. Forty-eight hours after the treatment and 48 h after each challenge (days −5, 2, 9, 16, 23, 30 and 37), the population of the remaining ticks was assessed for each animal. Geometric mean tick counts obtained were reduced by 99% and 94% on day 2 in groups 2 and 3, respectively, compared to the negative control group. Dogs were protected from re-infestations with an efficacy of >90% for 3 weeks in group 2 and for 5 weeks in group 3. Both 10% w/v fipronil-based spot-on solutions, despite different vehicles, were equally able to eradicate tick infestation, to prevent new infestations and were equally well tolerated.

Tài liệu tham khảo

Baxter C, Vogelnest L, Doggett S (2009) Dermatoses caused by infestations of immature Ixodes spp. on dogs and cats in Sydney, Australia. Aust Vet J 87:182–187 Beugnet F, Marie JL (2009) Emerging arthropod-borne diseases of companion animals in Europe. Vet Parasitol 163:298–305 Bonneau S, Fourier JJ, Rousseau C, Cadiergues MC (2010) Comparative efficacy of two fipronil spot-on formulations against experimental flea infestations (Ctenocephalides felis) in dogs. Intern J Appl Res Vet Med 8:16–20 Cruthers L, Slone RL, Guerrero AJ, Robertson-Plouch C (2001) Evaluation of the speed of kill of fleas and ticks with frontline top spot in dogs. Vet Ther 2:170–174 des Vignes F, Piesman J, Heffernan R, Schulze T, Stafford K III, Fish D (2001) Effect of tick removal on transmission of Borrelia burgdorferi and Ehrlichia phagocytophila by Ixodes scapularis nymps. J Infect Dis 183:773–778 Dryden MW, Payne PA, Smith V, Hosteder J (2006) Evaluation of an imidacloprid (8.8% w/w)-permethrin (44.0% w/w) topical spot-on and a fipronil (9.8% w/w)-(S)-methoprene (8.8% w/w) topical spot-on to repel, prevent attachment, and kill adult Ixodes scapularis and Amblyomma americanum ticks on dogs. Vet Ther 7:173–186 Fritz CL (2009) Emerging tick-borne diseases. Vet Clin N Am Small Anim Pract 39:265–278 Gray JS, Dautel H, Estrada-Pena A, Kahl O, Lindgren E (2009) Effects of climate change on ticks and tick-borne diseases in Europe. Interdiscip Perspect Infect Dis 2009:593232 Greene C (2006) Environmental factors in infectious disease. In: Greene C (ed) Infectious diseases of the dog and cat. Saunders Elsevier, St Louis, MI, pp 991–1012 Katavolos P, Armstrong P, Dawson J, Telford S III (1998) Duration of tick attachment required for transmission of granulocytic ehrlichiosis. J Infect Dis 177:1422–1425 Lindgren E, Talleklint L, Polfeldt T (2000) Impact of climatic change on the northern latitude limit and population density of the disease-transmitting European tick Ixodes ricinus. Environ Health Perspect 108:119–123 Piesman J, Dolan M (2002) Protection against Lyme disease spirochete transmission provided by prompt removal of nymphal Ixodes scapularis (Acari: Ixodidae). J Med Entomol 39:509–512 Pollmeier M, Pengo G, Jeannin P, Soll MD (2001) Evaluation of the efficacy of FRONTLINE (R) (active ingredient: Fipronil) in the treatment and control of European castor bean tick (Ixodes ricinus) infestations in the dog and cat. Kleintierpraxis 46:99–104 Postal JM, Longo F, Jeannin PC, Hunter JS, Tanner P, Keister DM, Romano M (1997) Efficacy against ticks (Rhipicephalus sanguineus) in dogs of two topical formulations of fipronil (Frontline spray and Frontline Top-Spot) after experimental infestation: an overall analysis of various studies. Fourth International Symposium on Ectoparasites of Pets. N. C. Hinkle. University of California, Riverside, CA, USA, pp 105–106 Schuele G, Barnett S, Bapst B, Cavaliero T, Luempert L, Strehlau G, Young DR, Moran C, Junquera P (2008) Efficacy of a single application of a 12.5% pyriprole topical solution against laboratory infestations with ticks (Ixodes ricinus, Dermacentor reticulatus, and Rhipicephalus sanguineus) on dogs. Vet Parasitol 154:311–317 Young DR, Arther RG, Davis WL (2003) Evaluation of K9 advantix (TM) vs. frontline plus (R) topical treatments to repel brown dog ticks (Rhipicephalus sanguineus) on dogs. Parasitol Res 90:S116–S118