Comparative analysis of multiple receptor subunit mRNA in micropunches obtained from different brain regions using a competitive RT-PCR method

Journal of Neuroscience Methods - Tập 84 - Trang 33-40 - 1998
Vijayender R Durgam1, Steven W Mifflin1
1Department of Pharmacology, University of Texas Health Science Center, 7703 Floyd Curl Dr., San Antonio, TX 78284-7764, USA

Tài liệu tham khảo

Celi, 1993, A rapid versatile method to synthesize internal standard for competitive PCR, Nucleic Acids Res, 21, 1047, 10.1093/nar/21.4.1047 Clementi, 1993, Quantitative PCR and RT-PCR in virology, PCR Methods Appl, 2, 191, 10.1101/gr.2.3.191 Comer, 1997, Detection of weekly expressed genes in the rostral ventrolateral medulla of the rat using micropunch and reverse transcription-polymerase chain reaction techniques, Clin Exp Pharm Physiol, 24, 755, 10.1111/j.1440-1681.1997.tb02127.x Lewohl, 1997, A method for the quantitation of the a1, a 2, and a 3 isoforms of the GABAA receptor in human brain using competitive PCR, Brain Res Protocols, 1, 347, 10.1016/S1385-299X(97)00009-3 Muller, 1996, Quantification of rat pre-pro-thyrotropin releasing hormone (TRH) mRNA by reverses transcription-polymerase chain reaction using external and internal standardisation, J Neurosci Methods, 68, 269, 10.1016/0165-0270(96)00090-8 Puia, 1991, Influence of recombinant GABAA receptor subunit composition on the actilvation of allosteric modulation of GABA gated Cl− currents, Mol Pharmacol, 39, 691 Segerson, 1987, Thyroid hormone regulates TRH biosynthesis in the paraventricular nucleus of the rat hypothalmus, Science, 238, 78, 10.1126/science.3116669 Verdoorn, 1990, Functional properties of recombinant rat GABAA receptors depend up on subunit composition, Neuron, 4, 919, 10.1016/0896-6273(90)90145-6 Yamada, 1990, Reciprocal regulation of preprothyrotropin-releasing hormone (TRH) mRNA in the rat anterior hypothalmus by thyroid hormone: disassociation from TRH concentrations during hypothathyroidism, Neuropeptides, 15, 49, 10.1016/0143-4179(90)90160-Z Zachar, 1993, Absolute quantification of target DNA: a simple competitive PCR for efficient analysis of multiple samples, Nucleic Acids Res, 21, 2017, 10.1093/nar/21.8.2017