So sánh Hồ sơ Biểu hiện Phiên mã của các Gen Phát triển Trichome của Đậu nành dưới Stress Muối

Plant Molecular Biology Reporter - Tập 36 - Trang 82-93 - 2017
Özge Çelik1, Çimen Atak1, Zekiye Suludere2
1Faculty of Science and Letters, Department of Molecular Biology and Genetics, İstanbul Kültür University, İstanbul, Turkey
2Faculty of Science, Department of Biology, Gazi University, Ankara, Turkey

Tóm tắt

Mục tiêu của nghiên cứu này là xác định sự thay đổi cấu trúc siêu vi và sự điều chỉnh của các gen liên quan đến chuyển hóa trichome dưới áp lực muối ở cây đậu nành (Glycine max L. Merr.). Các cây giống đậu nành Ataem-7 và S04-05 ở giai đoạn 14 ngày tuổi đã được xử lý với các nồng độ NaCl là 0, 50, 100 và 150 mM. Trong khi lượng diệp lục bị giảm, hoạt động của guaiacol peroxidase tăng lên ở cả hai giống do nồng độ NaCl tăng. Ở giống đậu nành S04-05, mật độ trichome gia tăng trên cả hai bề mặt lá, trong khi đó, mật độ trichome giảm ở giống Ataem-7 khi điều trị với 150 mM NaCl. Mật độ khí khổng gia tăng trên cả hai bề mặt lá của cả hai giống đậu nành sau khi chịu áp lực muối. Chúng tôi cũng đã thực hiện phân tích qRT-PCR để đánh giá mức độ phiên mã tương đối của các gen tương đồng với gen phát triển trichome ở Arabidopsis. Phân tích qRT-PCR cho thấy sự kích thích của các gen tương đồng GL2 và GL3 ở cây đậu nành sau khi điều trị với 50, 100 và 150 mM NaCl trong cả hai giống. Mặc dù mức độ biểu hiện của gen tương đồng TTG1 bị ảnh hưởng tiêu cực ở cả hai giống đậu nành dưới các nồng độ muối khác nhau, hồ sơ biểu hiện gen tương đồng GL1 lại khác nhau phụ thuộc vào việc thay đổi nồng độ muối ở cả hai giống so với cây đối chứng. Có thể nhận thấy rằng sự điều chỉnh hình thành trichome khác nhau giữa hai giống đậu nành.

Từ khóa

#Đậu nành #trichome #áp lực muối #gen phát triển #qRT-PCR

Tài liệu tham khảo

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