Combining intracellular and secreted bioluminescent reporter proteins for multicolor cell-based assays

Photochemical & Photobiological Sciences - Tập 7 Số 2 - Trang 212-217 - 2008
Elisa Michelini1,2, Luca Cevenini1,2, Laura Mezzanotte1,2, Danielle M. Ablamsky3, Tara L. Southworth3, Bruce R. Branchini3, Aldo Roda2
1Center for Applied Biomedical Research (CRBA), S. Orsola-Malpighi Hospital, Bologna, Italy
2Department of Pharmaceutical Sciences, University of Bologna, Italy
3Department of Chemistry, Connecticut College, New London, USA

Tóm tắt

Từ khóa


Tài liệu tham khảo

F. Fan, K. V. Wood, Bioluminescent assays for high-throughput screening Assay Drug Dev. Technol., 2007, 5, 127–36.

A. Roda, P. Pasini, M. Mirasoli, E. Michelini, M. Guardigli, Biotechnological applications of bioluminescence and chemiluminescence Trends Biotechnol., 2004, 22, 295–303.

E. Michelini, M. Magliulo, P. Leskinen, M. Virta, M. Karp, A. Roda, Recombinant cell-based bioluminescence assay for androgen bioactivity determination in clinical samples Clin. Chem., 2005, 51, 1995–8.

D. E. Nivens, T. E. McKnight, S. A. Moser, S. J. Osbourn, M. L. Simpson, G. S. Sayler, Bioluminescent bioreporter integrated circuits: potentially small, rugged and inexpensive whole-cell biosensors for remote environmental monitoring J. Appl. Microbiol., 2004, 96, 33–46.

D. O. Fesenko, T. V. Nasedkina, D. V. Prokopenko, A. D. Mirzabekov, Biosensing and monitoring of cell populations using the hydrogel bacterial microchip Biosens. Bioelectron., 2005, 20, 1860–5.

E. H. White, E. Rapaport, H. H. Seliger, T. A. Hopkins, The chemi- and bioluminescence of firefly luciferin: an efficient chemical production of electronically excited states Bioorg. Chem., 1971, 1, 92–122.

M. DeLuca, Firefly luciferase Adv. Enzymol., 1976, 44, 37–68.

S. S. Patterson, H. M. Dionisi, R. K. Gupta, G. S. Sayler, Codon optimization of bacterial luciferase (lux) for expression in mammalian cells J. Ind. Microbiol. Biotechnol., 2005, 32, 115–23.

H. Fujii, K. Noda, Y. Asami, A. Kuroda, M. Sakata, A. Tokida, Increase in bioluminescence intensity of firefly luciferase using genetic modification Anal. Biochem., 2007, 366, 131–6.

A. M. Loening, T. D. Fenn, A. M. Wu, S. S. Gambhir, Consensus guided mutagenesis of Renilla luciferase yields enhanced stability and light output Protein Eng. Des. Sel., 2006, 19, 391–400.

B. R. Branchini, T. L. Southworth, J. P. De Angelis, A. Roda, E. Michelini, Luciferase from the Italian firefly Luciola italica: Molecular cloning and expression Comp. Biochem. Physiol., B: Biochem. Mol. Biol., 2006, 145, 159–67.

V. R. Viviani, F. G. Arnoldi, M. Brochetto-Braga, Y. Ohmiya, Cloning and characterization of the cDNA for the Brazilian Cratomorphus distinctus larval firefly luciferase: similarities with European Lampyris noctiluca and Asiatic Pyrocoelia luciferases Comp. Biochem. Physiol., B: Biochem. Mol. Biol., 2004, 139, 151–6.

A. R. Emamzadeh, S. Hosseinkhani, M. Sadeghizadeh, M. Nikkhah, M. J. Chaichi, M. Mortazavi, cDNA cloning, expression and homology modeling of a luciferase from the firefly Lampyroidea maculata J. Biochem. Mol. Biol., 2006, 39, 578–85.

B. S. Alipour, S. Hosseinkhani, M. Nikkhah, H. Naderi-Manesh, M. J. Chaichi, S. K. Osaloo, Molecular cloning, sequence analysis, and expression of a cDNA encoding the luciferase from the glow-worm Lampyris turkestanicus Biochem. Biophys. Res. Commun., 2004, 325, 215–22.

S. V. Markova, S. Golz, L. A. Frank, B. Kalthof, E. S. Vysotski, Cloning and expression of cDNA for a luciferase from the marine copepod Metridia longa. A novel secreted bioluminescent reporter enzyme J. Biol. Chem., 2004, 279, 3212–7.

Y. Nakajima, T. Kimura, K. Sugata, T. Enomoto, A. Asakawa, H. Kubota, M. Ikeda, Y. Ohmiya, Multicolor luciferase assay system: one-step monitoring of multiple gene expressions with a single substrate Biotechniques, 2005, 38, 891–4.

K. Hakkila, M. Maksimow, M. Karp, M. Virta, Reporter genes lucFF, luxCDABE, gfp, and dsred have different characteristics in whole-cell bacterial sensors Anal. Biochem., 2002, 301, 235–42.

C. Wu, C. Suzuki-Ogoh, Y. Ohmiya, Dual-reporter assay using two secreted luciferase genes Biotechniques, 2007, 42, 290–292.

B. A. Tannous, D. E. Kim, J. L. Fernandez, R. Weissleder, X. O. Breakefield, Codon-optimized Gaussia luciferase cDNA for mammalian gene expression in culture and in vivo Mol. Ther., 2005, 11, 435–43.

T. Suzuki, S. Usuda, H. Ichinose, S. Inouye, Real-time bioluminescence imaging of a protein secretory pathway in living mammalian cells using Gaussia luciferase FEBS Lett., 2007, 581, 4551–6.

I. Remy, S. W. Michnick, A highly sensitive protein-protein interaction assay based on Gaussia luciferase Nat. Methods, 2006, 3, 977–9.

S. Wiles, K. Ferguson, M. Stefanidou, D. B. Young, B. D. Robertson, Alternative luciferase for monitoring bacterial cells under adverse conditions Appl. Environ. Microbiol., 2005, 71, 3427–32.

B. R. Branchini, D. M. Ablamsky, M. H. Murtiashaw, L. Uzasci, H. Fraga, T. L. Southworth, Thermostable red and green light-producing firefly luciferase mutants for bioluminescent reporter applications Anal. Biochem., 2007, 361, 253–62.

G. Campana, P. Pasini, A. Roda, S. Spampinato, Regulation of ileal bile acid-binding protein expression in Caco-2 cells by ursodeoxycholic acid: role of the farnesoid X receptor Biochem. Pharmacol., 2005, 69, 1755–63.

Chroma-Glo Luciferase Assay System, Technical Manual No. TM062, Promega Corporation, 2003, 7–9.

S. Knappskog, H. Ravneberg, C. Gjerdrum, C. Trösse, B. Stern, I. F. Pryme, The level of synthesis and secretion of Gaussia princeps luciferase in transfected CHO cells is heavily dependent on the choice of signal peptide J. Biotechnol., 2007, 128, 705–15.

J. Y. Chiang, R. Kimmel, C. Weinberger, D. Stroup, Farnesoid X receptor responds to bile acids and represses cholesterol 7alpha-hydroxylase gene (CYP7A1) transcription J. Biol. Chem., 2000, 275, 10918–24.

E. Michelini, L. Cevenini, L. Mezzanotte, D. Ablamsky, T. Southworth, B. R. Branchini, A. Roda, Spectral-resolved gene technology for multiplexed bioluminescence and high content screening Anal. Chem., 2008, 80, 260–7.