Combined MRI–PET dissects dynamic changes in plant structures and functions

Plant Journal - Tập 59 Số 4 - Trang 634-644 - 2009
Siegfried Jahnke1,2, Marion I. Menzel2, Dagmar van Dusschoten2, Gerhard W. Roeb2, Jonas Bühler2, Senay Minwuyelet2, Peter Blümler2, Vicky M. Temperton2, Thomas Hombach2, M. Streun3, Simone Beer3, Maryam Khodaverdi3, K. Ziemons3, Heinz H. Coenen4, Ulrich Schurr2
1FB Biologie und Geographie, Universität Duisburg-Essen, 45117 Essen, Germany
2ICG-3: Phytosphere, Forschungszentrum Jülich, 52425 Jülich, Germany
3ZEL: Central Institute for Electronics, Forschungszentrum Jülich, 52425 Jülich, Germany
4INB-5: Nuclear Chemistry, Forschungszentrum Jülich, 52425 Jülich, Germany

Tóm tắt

SummaryUnravelling the factors determining the allocation of carbon to various plant organs is one of the great challenges of modern plant biology. Studying allocation under close to natural conditions requires non‐invasive methods, which are now becoming available for measuring plants on a par with those developed for humans. By combining magnetic resonance imaging (MRI) and positron emission tomography (PET), we investigated three contrasting root/shoot systems growing in sand or soil, with respect to their structures, transport routes and the translocation dynamics of recently fixed photoassimilates labelled with the short‐lived radioactive carbon isotope 11C. Storage organs of sugar beet (Beta vulgaris) and radish plants (Raphanus sativus) were assessed using MRI, providing images of the internal structures of the organs with high spatial resolution, and while species‐specific transport sectoralities, properties of assimilate allocation and unloading characteristics were measured using PET. Growth and carbon allocation within complex root systems were monitored in maize plants (Zea mays), and the results may be used to identify factors affecting root growth in natural substrates or in competition with roots of other plants. MRI–PET co‐registration opens the door for non‐invasive analysis of plant structures and transport processes that may change in response to genomic, developmental or environmental challenges. It is our aim to make the methods applicable for quantitative analyses of plant traits in phenotyping as well as in understanding the dynamics of key processes that are essential to plant performance.

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Tài liệu tham khảo

10.1146/annurev.py.26.090188.001251

Artschwager E., 1926, Anatomy of the vegetative organs of the sugar beet, J. Agric. Res., 33, 143

10.1093/jxb/erm294

10.1126/science.290.5491.521

10.1007/978-0-387-22529-6_1

10.1002/pmic.200700744

10.1016/0883-2889(87)90123-7

10.1088/0031-9155/51/13/R02

10.1007/BF02357889

10.1007/s00425-008-0701-9

Hayward H.E., 1938, The Structure of Economic Plants

10.1016/B978-012088457-5/50001-0

10.1007/BF02411410

10.1093/jxb/15.3.485

10.1038/nm1700

10.1109/TNS.2006.881063

10.1104/pp.125.4.1743

10.1111/j.0022-2720.2004.01351.x

De Kroon H., 2007, How do roots interact?, Science, 318, 1562, 10.1126/science.1150726

10.1016/S0969-8043(97)00117-6

10.1007/978-3-642-60125-5_50

MacFall J.S., 1996, Encyclopedia of Nuclear Magnetic Resonance, 3633

10.1016/S1161-0301(01)00139-3

10.1073/pnas.0707368104

10.1146/annurev.arplant.50.1.695

10.1111/j.1744-7348.1976.tb00604.x

10.1002/9780470751114.ch3

10.1111/j.1469-8137.2005.01323.x

10.1071/FP03008

10.1111/j.1468-1331.2007.01997.x

10.1002/9780470751114.ch10

10.1109/TNS.2005.862958

10.1080/00380768.2002.10409267

10.1023/A:1022252419649

10.1126/science.1147243

10.3732/ajb.89.2.270

10.1146/annurev.arplant.58.032806.104006

10.1007/978-0-387-22529-6

Phelps M.E., 1975, Effect of positron range on spatial resolution, J. Nucl. Med., 16, 649

10.1093/jxb/44.9.1491

10.2136/vzj2008.0030

10.1111/j.1745-4557.2005.00046.x

10.1016/S0006-3495(02)75413-1

10.1073/pnas.0602316103

10.1126/science.272.5267.1423

10.1016/S0044-328X(76)80034-7

10.1109/TNS.2006.875051

10.1007/s00425-007-0503-5

10.1093/pcp/pcn192

10.1046/j.0016-8025.2003.01068.x

10.1016/j.nima.2006.08.048

10.1111/j.1365-3040.2006.01544.x

10.1017/S002185969700498X

10.1016/S0262-8856(03)00137-9