Combinatorial peptide libraries to overcome the classical affinity-enrichment methods in proteomics

Amino Acids - Tập 45 - Trang 219-229 - 2013
Pier Giorgio Righetti1, Egisto Boschetti2
1Miles Gloriosus Academy, Milan, Italy
2Scientific Consultant, Paris, France

Tóm tắt

The enrichment of targeted low-abundance proteins is possible by affinity adsorption using selected sorbents. Different categories of very dilute proteins are present in most of biological extracts so that specific affinity methods are unable to address their collective enrichment. Only recently an interesting approach has been proposed associating the affinity of multiple ligands used as a library mode under overloading much beyond the saturation of the affinity mixed bed. The principle and the limits of this technology are reported along with their current and potential applications in various domains.

Tài liệu tham khảo

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