Combination of commercially available molecular assays and culture based methods in diagnosis of tuberculosis and drug resistant tuberculosis

European Centre for Disease Prevention and Control/WHO Regional Office for Europe, 2014 Papaventsis, 2010, Tuberculosis in Greece: bacteriologically confirmed cases and anti-tuberculosis drug resistance, 1995–2009, Euro Surveill, 15, 10.2807/ese.15.28.19614-en Jelastopulu, 2009, Substantial underreporting of tuberculosis in West Greece: implications for local and national surveillance, Euro Surveill, 14, 10.2807/ese.14.11.19152-en Smith, 2006, A&E department: a missed opportunity for diagnosis of TB?, Thorax, 61, 364, 10.1136/thx.2005.053637 Drobniewski, 2013, Rapid diagnostics of tuberculosis and drug resistance in the industrialized world: clinical and public health benefits and barriers to implementation, BMC Med, 11, 190, 10.1186/1741-7015-11-190 Blumberg, 2013, American Thoracic Society/Centers for Disease Control and Prevention/Infectious Diseases Society of America: treatment of tuberculosis, Am J Respir Crit Care Med, 167, 603 Rovina, 2011, MIRU-VNTR typing of drug-resistant tuberculosis isolates in Greece, Ther Adv Respir Dis, 5, 229, 10.1177/1753465811402120 Bwanga, 2009, Direct susceptibility testing for multi drug resistant tuberculosis: a meta-analysis, BMC Infect Dis, 9, 67, 10.1186/1471-2334-9-67 Bloemberg, 2013, Evaluation of Cobas TaqMan MTB for direct detection of the Mycobacterium tuberculosis complex in comparison with Cobas Amplicor MTB, J Clin Microbiol, 51, 2112, 10.1128/JCM.00142-13 Cho, 2015, Comparison of AdvanSure TB/NTM PCR and COBAS TaqMan MTB PCR for detection of Mycobacterium tuberculosis complex in routine clinical practice, Ann Lab Med, 35, 356, 10.3343/alm.2015.35.3.356 Tortoli, 2012, Is real-time PCR better than conventional PCR for Mycobacterium tuberculosis complex detection in clinical samples?, J Clin Microbiol, 50, 2810, 10.1128/JCM.01412-12 Fegou, 2005, Sensitivity of the Cobas Amplicor system for detection of Mycobacterium tuberculosis in respiratory and extrapulmonary specimens, Clin Microbiol Infect, 11, 593, 10.1111/j.1469-0691.2005.01185.x Wang, 1999, Evaluation of three nucleic acid amplification methods for direct detection of Mycobacterium tuberculosis complex in respiratory specimens, J Clin Microbiol, 37, 1932, 10.1128/JCM.37.6.1932-1934.1999 Yang, 2011, Evaluation of the Cobas TaqMan MTB test for direct detection of Mycobacterium tuberculosis complex in respiratory specimens, J Clin Microbiol, 49, 797, 10.1128/JCM.01839-10 Huh, 2015, Comparison of the genedia MTB detection kit and the cobas TaqMan MTB assay for detection of Mycobacterium tuberculosis in respiratory specimens, J Clin Microbiol, 53, 1012101 Kim, 2011, Evaluation of Cobas TaqMan MTB PCR for detection of Mycobacterium tuberculosis, J Clin Microbiol, 49, 173, 10.1128/JCM.00694-10 Cohen, 1998, Diagnosis of pulmonary tuberculosis using PCR assays on sputum collected within 24hours of hospital admission, Am J Respir Crit Care Med, 157, 156, 10.1164/ajrccm.157.1.97-06043 Panaiotov, 2010, Dynamics of the laboratory results in patients with pulmonary tuberculosis, Diagn Microbiol Infect Dis, 67, 327, 10.1016/j.diagmicrobio.2010.03.002 Fegou, 2006, Comparison of the manual Mycobacteria Growth Indicator tube and the Etest with the method of proportion for susceptibility testing of Mycobacterium tuberculosis, Chemotherapy, 52, 174, 10.1159/000093035 Banu, 2014, Discordance across several methods for drug susceptibility testing of drug-resistant Mycobacterium tuberculosis isolates in a single laboratory, J Clin Microbiol, 52, 156, 10.1128/JCM.02378-13 Matsiota-Bernard, 1998, Characterization of rpoB mutations in rifampin-resistant clinical Mycobacterium tuberculosis isolates from Greece, J Clin Microbiol, 36, 20, 10.1128/JCM.36.1.20-23.1998 Gitti, 2011, GenoType(R) MTBDRplus compared with conventional drug-susceptibility testing of Mycobacterium tuberculosis in a low-resistance locale, Future Microbiol, 6, 357, 10.2217/fmb.11.8