Collagen–chitosan polymer as a scaffold for the proliferation of human adipose tissue-derived stem cells

Springer Science and Business Media LLC - Tập 20 - Trang 799-808 - 2008
Yanxia Zhu1, Tianqing Liu1, Kedong Song1, Bo Jiang1, Xuehu Ma1, Zhanfeng Cui2
1Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian, China
2Department of Engineering Science, Oxford Centre for Tissue Engineering and Bioprocessing, Oxford University, Oxford, UK

Tóm tắt

The architecture and biomaterial are vital for three-dimensional culture of cells in scaffolds, so collagen–chitosan scaffolds suitable for the proliferation of adipose tissue-derived stem cells (ADSCs) were fabricated in this study. Chitosan was fully mixed with collagen with different volume ratio and cross-linked. The microstructure, pore size, bibulous ability, water content, interval porosity, enzyme degradation and affinity were examined before and after cross-linking. During ADSCs cultured in scaffold, the viability and metabolic rates were measured. After 14 days, the surface markers, specific transcription factors and multi-differentiation potential were assayed to identify the stemness of expanded cells. According to the pore size, bibulous ability, interval porosity, degradation rate and affinity of the scaffold, we chose cross-linked scaffolds of 7:3 material ratio as a better scaffold for ADSCs proliferation, and ADSCs could be expanded by more than 20 times. All expanded cells still maintained stem cell characteristics and pluripotency. So our developed collagen–chitosan scaffolds can promote ADSCs adhesion, expansion, and maintain pluripotency.

Tài liệu tham khảo