Collagen at interfaces I. In situ collagen adsorption at solution/air and solution/polymer interfaces

Wiley - Tập 20 Số 7 - Trang 951-962 - 1986
Michel Deyme1, Adam Baszkin1, J. E. Proust1, Éric Perez1, Marie Martine Boissonnade1
1Physico‐Chimie des Surfaces et Innovation en Pharmacotechnie UA CNRS 1218, Université Paris‐Sud, 5 rue J. B. Clément 92290 Chǎtenay‐Malabry, France

Tóm tắt

AbstractCollagen was isolated from rat tail tendons and acetylated with 1–14C acetic anhydride. In situ adsorption of this collagen from a buffer solution (pH = 2.7) was measured at the interfaces to air, polyethylene and polyethylene grafted with poly(maleic acid), respectively.The kinetics of adsorption were recorded for all surfaces studied and the corresponding diffusion coefficients for collagen in solution with various protein concentrations were calculated. The desorption of collagen from polymer surfaces was also studied.These experiments reveal the existence of both a reversibly and an irreversibly adsorbed collagen layer on the polymers tested. The desorption/adsorption ratio for the polyethylene is higher than that for the grafted polyethylene indicating stronger interactions of collagen with the grafted surface than with the non‐modified polyethylene.

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Wiley

Tập 20 Số 7

951-962

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