Co-expression of functional human Heme Oxygenase 1, Ecto-5′-Nucleotidase and ecto-nucleoside triphosphate diphosphohydrolase-1 by “self-cleaving” 2A peptide system

Akamatsu, 2004, Heme oxygenase-1-derived carbon monoxide protects hearts from transplant associated ischemia reperfusion injury, FASEB J., 18, 771, 10.1096/fj.03-0921fje Antonioli, 2013, CD39 and CD73 in immunity and inflammation, Trends Mol. Med, 19, 355, 10.1016/j.molmed.2013.03.005 Arber, 2013, The immunogenicity of virus-derived 2A sequences in immunocompetent individuals, Gene Ther, 20, 958, 10.1038/gt.2013.25 Cooper, 2012, Clinical lung xenotransplantation–what donor genetic modifications may be necessary?, Xenotransplantation, 19, 144, 10.1111/j.1399-3089.2012.00708.x Cooper, 2013, The potential of genetically-engineered pigs in providing an alternative source of organs and cells for transplantation, J. Biomed. Res, 27, 249, 10.7555/JBR.27.20130063 Cozzi, 1997, Characterization of pigs transgenic for human decay-accelerating factor, Transplantation, 64, 1383, 10.1097/00007890-199711270-00002 de Felipe, 2004, Targeting of proteins derived from self-processing polyproteins containing multiple signal sequences, Traffic, 5, 616, 10.1111/j.1398-9219.2004.00205.x de Felipe, 1999, Use of the 2A sequence from foot-and-mouth disease virus in the generation of retroviral vectors for gene therapy, Gene Ther, 6, 198, 10.1038/sj.gt.3300811 de Felipe, 2006, E unum pluribus: multiple proteins from a self-processing polyprotein, Trends Biotechnol, 24, 68, 10.1016/j.tibtech.2005.12.006 De Giorgi, 2014, Functional analysis of expression of human ecto-nucleoside triphosphate diphosphohydrolase-1 and/or ecto-5′-nucleotidase in pig endothelial cells, Nucleosides Nucleotides Nucleic Acids, 33, 313, 10.1080/15257770.2014.896466 Deng, 2011, Use of the 2A peptide for generation of multi-transgenic pigs through a single round of nuclear transfer, PLoS ONE, 6, e19986, 10.1371/journal.pone.0019986 Di Stasi, 2011, Inducible apoptosis as a safety switch for adoptive cell therapy, N. Engl. J. Med, 365, 1673, 10.1056/NEJMoa1106152 Dwyer, 2004, Thromboregulatory manifestations in human CD39 transgenic mice and the implications for thrombotic disease and transplantation, J. Clin. Invest, 113, 1440, 10.1172/JCI19560 Fang, 2005, Stable antibody expression at therapeutic levels using the 2A peptide, Nat. Biotechnol, 23, 584, 10.1038/nbt1087 Fisicaro, 2011, Versatile co-expression of graft-protective proteins using 2A-linked cassettes, Xenotransplantation, 18, 121, 10.1111/j.1399-3089.2011.00631.x Gock, 2011, Genetic modification of pigs for solid organ xenotransplantation, Transplant. Rev. (Orlando), 25, 9, 10.1016/j.trre.2010.10.001 Gottlieb, 2012, Endoplasmic reticulum anchored heme-oxygenase 1 faces the cytosol, Haematologica, 97, 1489, 10.3324/haematol.2012.063651 Hasegawa, 2008, Ecto-5′ nucleotidase (CD73)-mediated adenosine generation and signaling in murine cardiac allograft vasculopathy, Circ. Res, 103, 1410, 10.1161/CIRCRESAHA.108.180059 Huttinger, 2012, Ectonucleotide triphosphate diphosphohydrolase-1 (CD39) mediates resistance to occlusive arterial thrombus formation after vascular injury in mice, Am. J. Pathol, 181, 322, 10.1016/j.ajpath.2012.03.024 Kaczmarek, 1996, Identification and characterization of CD39/vascular ATP diphosphohydrolase, J. Biol. Chem, 271, 33116, 10.1074/jbc.271.51.33116 Klemens, 1990, Characterization of soluble vs membrane-bound human placental 5′-nucleotidase, Biochem. Biophys. Res. Commun, 172, 1371, 10.1016/0006-291X(90)91601-N Klemz, 2009, Amplifying the fluorescence of bilirubin enables the real-time detection of heme oxygenase activity, Free Radic. Biol. Med, 46, 305, 10.1016/j.freeradbiomed.2008.10.044 Lavitrano, 2002, Efficient production by sperm-mediated gene transfer of human decay accelerating factor (hDAF) transgenic pigs for xenotransplantation, Proc. Natl. Acad. Sci. U.S.A., 99, 14230, 10.1073/pnas.222550299 Mansouri, 2014, Strategies for multigene expression in eukaryotic cells, Plasmid, 75, 12, 10.1016/j.plasmid.2014.07.001 Minskaia, 2013, Optimisation of the foot-and-mouth disease virus 2A co-expression system for biomedical applications, BMC Biotechnol, 13, 67, 10.1186/1472-6750-13-67 Misumi, 1990, Primary structure of human placental 5′-nucleotidase and identification of the glycolipid anchor in the mature form, Eur. J. Biochem, 191, 563, 10.1111/j.1432-1033.1990.tb19158.x Osborne, 2005, Expression of human ecto-5′-nucleotidase in pig endothelium increases adenosine production and protects from NK cell-mediated lysis, Am. J. Transplant, 5, 1248, 10.1111/j.1600-6143.2005.00868.x Park, 2014, Production and characterization of soluble human TNFRI-Fc and human HO-1 (HMOX1) transgenic pigs by using the F2A peptide, Transgenic Res, 23, 407, 10.1007/s11248-013-9780-x Petersen, 2011, Transgenic expression of human heme oxygenase-1 in pigs confers resistance against xenograft rejection during ex vivo perfusion of porcine kidneys, Xenotransplantation, 18, 355, 10.1111/j.1399-3089.2011.00674.x Rothwell, 2010, Functional expression of secreted proteins from a bicistronic retroviral cassette based on FMDV 2A can be position- dependent, Hum. Gene Ther, 21, 1631, 10.1089/hum.2009.197 Smolenski, 1990, Determination of sixteen nucleotides, nucleosides and bases using high-performance liquid chromatography and its application to the study of purine metabolism in hearts for transplantation, J. Chromatogr, 527, 414, 10.1016/S0378-4347(00)82125-8 Soares, 2009, Heme oxygenase-1: from biology to therapeutic potential, Trends Mol. Med, 15, 50, 10.1016/j.molmed.2008.12.004 Soares, 2004, Heme oxygenase-1 modulates the expression of adhesion molecules associated with endothelial cell activation, J. Immunol, 172, 3553, 10.4049/jimmunol.172.6.3553 Verrier, 2011, Bicistronic lentiviruses containing a viral 2A cleavage sequence reliably co-express two proteins and restore vision to an animal model of LCA1, PLoS ONE, 6, e20553, 10.1371/journal.pone.0020553 Yang, 2008, Development of optimal bicistronic lentiviral vectors facilitates high-level TCR gene expression and robust tumor cell recognition, Gene Ther, 15, 1411, 10.1038/gt.2008.90 Yin, 2005, Investigations of the effect of DNA size in transient transfection assay using dual luciferase system, Anal. Biochem, 346, 289, 10.1016/j.ab.2005.08.029 Zernecke, 2006, CD73/ecto-5′-nucleotidase protects against vascular inflammation and neointima formation, Circulation, 113, 2120, 10.1161/CIRCULATIONAHA.105.595249