Cloning, expression, and characterization of a membrane progestin receptor and evidence it is an intermediary in meiotic maturation of fish oocytes

Proceedings of the National Academy of Sciences of the United States of America - Tập 100 Số 5 - Trang 2231-2236 - 2003
Yong Zhu1, Charles D. Rice1, Yefei Pang1, Margaret C. Pace1, Peter Thomas1
1Marine Science Institute, University of Texas at Austin, 750 Channel View Drive, Port Aransas, TX 78373; Department of Biology, East Carolina University, 1000 East Fifth Street, Greenville, NC 27858; and Departments of Environmental Toxicology and Bioscience, Clemson University, 509 Westinghouse Road, Pendleton, SC 29670

Tóm tắt

The structures of membrane receptors mediating rapid, nongenomic actions of steroids have not been identified. We describe the cloning of a cDNA from spotted seatrout ovaries encoding a protein that satisfies the following seven criteria for its designation as a steroid membrane receptor: plausible structure, tissue specificity, cellular distribution, steroid binding, signal transduction, hormonal regulation, and biological relevance. For plausible structure, computer modeling predicts that the protein has seven transmembrane domains, typical of G protein-coupled receptors. The mRNA (4.0 kb) is only detected in the brain and reproductive tissues on Northern blots. Antisera only detect the protein (40 kDa) in plasma membranes of reproductive tissues. The recombinant protein produced in an Escherichia coli expression system has a high affinity ( K d = 30 nM), saturable, displaceable, single binding site specific for progestins. Progestins alter signal transduction pathways, activating mitogen-activated protein kinase and inhibiting adenylyl cyclase, in a transfected mammalian cell line. Inhibition of adenylyl cyclase is pertussis toxin sensitive, suggesting the receptor may be coupled to an inhibitory G protein. Progestins and gonadotropin up-regulate both mRNA and protein levels in seatrout ovaries. Changes in receptor abundance in response to hormones and at various stages of oocyte development, its probable coupling to an inhibitory G protein and inhibition of progestin induction of oocyte maturation upon microinjection of antisense oligonucleotides are consistent with the identity of the receptor as an intermediary in oocyte maturation. These characteristics suggest the fish protein is a membrane progestin receptor mediating a “nonclassical” action of progestins to induce oocyte maturation in fish.

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Thông tin xuất bản

Proceedings of the National Academy of Sciences of the United States of America

Tập 100 Số 5

2231-2236

Thông tin tác giả