Cloning and characterization of the N-methyl-D-aspartate receptor subunit NR1 gene from chum salmon, Oncorhynchus keta (Walbaum, 1792)

SpringerPlus - Tập 3 - Trang 1-8 - 2014
Jeong-Nam Yu1, Seung Hyub Ham2, Seung Il Lee2, Hyung-Joo Jin2, Hiroshi Ueda3, Deuk-Hee Jin2
1National Institute of Biological Resources, Incheon, Korea
2Department of Marine Molecular Biotechnology, Gangneung-Wonju National University, Gangneung, Korea
3Laboratory of Aquatic Bioresources and Ecosystem, Field Science Center for Northern Biosphere, Hokkaido University, Sapporo, Japan

Tóm tắt

Here, we report the information about molecular and expression characterization of NR1 gene in chum salmon for the first time. The complete NR1 subunit showed a large open-reading frame of 2844 bp in the total length of 3193 bp, and this cDNA contained a coding region encoding 948 amino acids and a stop codon. The organization of the NR1 subunit of chum salmon were similar of most other fishes, except C’ terminal. The expression of NR1 subunit was to show higher in the natal river near to the hatchery than near to the coast. We expect that the information reported herein may facilitate further investigations on the relationship between memory factors of natal rivers and homing mechanisms in Salmonidae.

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