Clinicopathologic study of E-cadherin/beta-catenin complex, and topoisomerase-II in a series of 71 liposarcoma cases
Tóm tắt
To investigate the expression of E-cadherin, beta-catenin and topoisomerase-II alpha and examine their clinical relevance in liposarcomas. The expression of E-cadherin, beta-catenin and topoisomerase II alpha was examined immunohistochemically on formalin-fixed paraffin-embedded tissue specimens from 71 patients who underwent surgical treatment for liposarcomas of the extremities or the retroperitoneum in two major cancer reference centres between 1990 and 2000. Detailed medical notes were available for all patients who were followed for median 82 months (range 5 to 215 months). Obtained expression data were weighted against clinical and pathology parameters of clinical relevance. Patients were mostly male (59%), median age was 56 years for the liposarcomas of the extremities and 60 years for the retroperitoneal liposarcomas. The tumours were of diverse histology, grade and size (median diameters 7 and 17 cm for tumours of the extremities and retroperitoneum respectively). Expression of β-catenin protein was weakly detected in 15 cases (21.1%). Similarly weak expression of topoisomerase II-alpha was detected in 14 (19.7%) cases of which only two had more than 20% of tumor cells stained positive. E-cadherin was not detected in the studied cohort of liposarcomas. We did not detect associations between the expression of the above proteins by liposarcoma cells and clinical outcome. Liposarcomas do not express E-cadherin, which matches the absence of epithelioid differentiation in this sarcoma subtype, and have low topoisomerase II-alpha expression, which justifies to some extend their resistance to anthracycline-based chemotherapy.
Tài liệu tham khảo
citation_journal_title=Virchows Arch; citation_title=Well-differentiated and dedifferentiated liposarcomas; citation_author=JM Coindre, F Pedeutour, A Aurias; citation_volume=456; citation_issue=2; citation_publication_date=2010; citation_pages=167-179; citation_doi=10.1007/s00428-009-0815-x; citation_id=CR1
citation_journal_title=N Engl J Med; citation_title=Soft-tissue sarcomas in adults; citation_author=MA Clark, C Fisher, I Judson, JM Thomas; citation_volume=353; citation_issue=7; citation_publication_date=2005; citation_pages=701-711; citation_doi=10.1056/NEJMra041866; citation_id=CR2
citation_journal_title=Histopathology; citation_title=The evolving classification of soft tissue tumours: an update based on the new WHO classification; citation_author=CD Fletcher; citation_volume=48; citation_issue=1; citation_publication_date=2006; citation_pages=3-12; citation_doi=10.1111/j.1365-2559.2005.02284.x; citation_id=CR3
citation_journal_title=Br J Radiol; citation_title=Radiotherapy of liposarcomas; citation_author=JB Reitan, O Kaalhus; citation_volume=53; citation_issue=634; citation_publication_date=1980; citation_pages=969-975; citation_doi=10.1259/0007-1285-53-634-969; citation_id=CR4
citation_journal_title=Cancer; citation_title=Significant clinical benefit of first-line palliative chemotherapy in advanced soft-tissue sarcoma: retrospective analysis and identification of prognostic factors in 488 patients; citation_author=V Karavasilis, BM Seddon, S Ashley, O Al-Muderis, C Fisher, I Judson; citation_volume=112; citation_issue=7; citation_publication_date=2008; citation_pages=1585-1591; citation_doi=10.1002/cncr.23332; citation_id=CR5
citation_journal_title=Cancer Res; citation_title=Microarray-based copy number and expression profiling in dedifferentiated and pleomorphic liposarcoma; citation_author=B Fritz, F Schubert, G Wrobel, C Schwaenen, S Wessendorf, M Nessling, C Korz, RJ Rieker, K Montgomery, R Kucherlapati; citation_volume=62; citation_issue=11; citation_publication_date=2002; citation_pages=2993-2998; citation_id=CR6
citation_journal_title=Hum Pathol; citation_title=Expression of E-cadherin in bone and soft tissue sarcomas: a possible role in epithelial differentiation; citation_author=H Sato, T Hasegawa, Y Abe, H Sakai, S Hirohashi; citation_volume=30; citation_issue=11; citation_publication_date=1999; citation_pages=1344-1349; citation_doi=10.1016/S0046-8177(99)90066-7; citation_id=CR7
citation_journal_title=Sci STKE; citation_title=Wnt/beta-catenin pathway; citation_author=RT Moon; citation_volume=2005; citation_issue=271; citation_publication_date=2005; citation_pages=cm1; citation_id=CR8
citation_journal_title=Science; citation_title=Convergence of Wnt, beta-catenin, and cadherin pathways; citation_author=WJ Nelson, R Nusse; citation_volume=303; citation_issue=5663; citation_publication_date=2004; citation_pages=1483-1487; citation_doi=10.1126/science.1094291; citation_id=CR9
citation_journal_title=Oncogene; citation_title=Cadherins and cancer: how does cadherin dysfunction promote tumor progression?; citation_author=A Jeanes, CJ Gottardi, AS Yap; citation_volume=27; citation_issue=55; citation_publication_date=2008; citation_pages=6920-6929; citation_doi=10.1038/onc.2008.343; citation_id=CR10
citation_journal_title=Clin Cancer Res; citation_title=Expression of E-cadherin and beta-catenin in human non-small cell lung cancer and the clinical significance; citation_author=S Kase, K Sugio, K Yamazaki, T Okamoto, T Yano, K Sugimachi; citation_volume=6; citation_issue=12; citation_publication_date=2000; citation_pages=4789-4796; citation_id=CR11
citation_journal_title=Nat Rev Cancer; citation_title=DNA topoisomerase II and its growing repertoire of biological functions; citation_author=JL Nitiss; citation_volume=9; citation_issue=5; citation_publication_date=2009; citation_pages=327-337; citation_doi=10.1038/nrc2608; citation_id=CR12
citation_journal_title=J Clin Oncol; citation_title=Topoisomerase II{alpha} amplification and anthracycline-based chemotherapy: the jury is still out; citation_author=FJ Esteva, GN Hortobagyi; citation_volume=27; citation_issue=21; citation_publication_date=2009; citation_pages=3416-3417; citation_doi=10.1200/JCO.2009.22.6449; citation_id=CR13
citation_journal_title=Nat Rev Cancer; citation_title=Targeting DNA topoisomerase II in cancer chemotherapy; citation_author=JL Nitiss; citation_volume=9; citation_issue=5; citation_publication_date=2009; citation_pages=338-350; citation_doi=10.1038/nrc2607; citation_id=CR14
citation_journal_title=J BUON; citation_title=E-cadherin, b-catenin and topoisomerase II expression in rhabdomyosarcomas; citation_author=PN Gogou, A Batistatou, EE Pakos, N Apostolikas, D Stefanou, PG Tsekeris; citation_volume=14; citation_issue=2; citation_publication_date=2009; citation_pages=323-324; citation_id=CR15
citation_journal_title=Arch Pathol Lab Med; citation_title=Expression of E-cadherin and p53 proteins in human soft tissue sarcomas; citation_author=J Yoo, S Park, CS Kang, SJ Kang, BK Kim; citation_volume=126; citation_issue=1; citation_publication_date=2002; citation_pages=33-38; citation_id=CR16
citation_journal_title=Mod Pathol; citation_title=Nuclear beta-catenin in mesenchymal tumors; citation_author=TL Ng, AM Gown, TS Barry, MC Cheang, AK Chan, DA Turbin, FD Hsu, RB West, TO Nielsen; citation_volume=18; citation_issue=1; citation_publication_date=2005; citation_pages=68-74; citation_doi=10.1038/modpathol.3800272; citation_id=CR17
citation_journal_title=Arch Pathol Lab Med; citation_title=Beta-catenin accumulation and gene mutation in exon 3 in dedifferentiated liposarcoma and malignant fibrous histiocytoma; citation_author=A Sakamoto, Y Oda, T Adachi, T Saito, S Tamiya, Y Iwamoto, M Tsuneyoshi; citation_volume=126; citation_issue=9; citation_publication_date=2002; citation_pages=1071-1078; citation_id=CR18
citation_journal_title=Cell; citation_title=Wnt/beta-catenin signaling in development and disease; citation_author=H Clevers; citation_volume=127; citation_issue=3; citation_publication_date=2006; citation_pages=469-480; citation_doi=10.1016/j.cell.2006.10.018; citation_id=CR19
citation_journal_title=Hum Pathol; citation_title=Prognostic significance of histologic grade and nuclear expression of beta-catenin in synovial sarcoma; citation_author=T Hasegawa, R Yokoyama, Y Matsuno, T Shimoda, S Hirohashi; citation_volume=32; citation_issue=3; citation_publication_date=2001; citation_pages=257-263; citation_doi=10.1053/hupa.2001.22764; citation_id=CR20
citation_journal_title=Histopathology; citation_title=Uterine sarcomas in Norway. A histopathological and prognostic survey of a total population from 1970 to 2000 including 419 patients; citation_author=VM Abeler, O Royne, S Thoresen, HE Danielsen, JM Nesland, GB Kristensen; citation_volume=54; citation_issue=3; citation_publication_date=2009; citation_pages=355-364; citation_doi=10.1111/j.1365-2559.2009.03231.x; citation_id=CR21
citation_journal_title=J Thorac Cardiovasc Surg; citation_title=Reduced membranous beta-catenin protein expression is associated with metastasis and poor prognosis in squamous cell carcinoma of the esophagus; citation_author=PK Hsu, AF Li, YC Wang, CC Hsieh, MH Huang, WH Hsu, HS Hsu; citation_volume=135; citation_issue=5; citation_publication_date=2008; citation_pages=1029-1035; citation_doi=10.1016/j.jtcvs.2007.11.007; citation_id=CR22
citation_journal_title=Mod Pathol; citation_title=Low membranous expression of beta-catenin and high mitotic count predict poor prognosis in endometrioid carcinoma of the ovary; citation_author=DG Rosen, Z Zhang, B Chang, X Wang, E Lin, J Liu; citation_volume=23; citation_issue=1; citation_publication_date=2010; citation_pages=113-122; citation_doi=10.1038/modpathol.2009.141; citation_id=CR23
citation_journal_title=Clin Transl Oncol; citation_title=Expression of E-cadherin, beta-catenin and topoisomerase IIalpha in leiomyosarcomas; citation_author=PN Gogou, A Batistatou, EE Pakos, N Apostolikas, D Stefanou, PG Tsekeris; citation_volume=11; citation_issue=8; citation_publication_date=2009; citation_pages=548-551; citation_doi=10.1007/s12094-009-0401-3; citation_id=CR24
citation_title=DNA Topoisomerase II Enzymes as Molecular Targets for Cancer Chemotherapy; citation_inbook_title=Curr Cancer Drug Targets; citation_publication_date=2010; citation_id=CR25; citation_author=K Chikamori; citation_author=AG Grozav; citation_author=T Kozuki; citation_author=D Grabowski; citation_author=R Ganapathi; citation_author=MK Ganapathi
citation_journal_title=Breast Cancer Res Treat; citation_title=Gene expression of topoisomerase II alpha (TOP2A) by microarray analysis is highly prognostic in estrogen receptor (ER) positive breast cancer; citation_author=A Rody, T Karn, E Ruckhaberle, V Muller, M Gehrmann, C Solbach, A Ahr, R Gatje, U Holtrich, M Kaufmann; citation_volume=113; citation_issue=3; citation_publication_date=2009; citation_pages=457-466; citation_doi=10.1007/s10549-008-9964-x; citation_id=CR26
citation_journal_title=Pathol Int; citation_title=Unique cell membrane expression of topoisomerase-II alpha as a useful diagnostic marker of liposarcoma; citation_author=H Endo, M Hirokawa, N Ishimaru, Y Tanaka, M Yamashita, M Sakaki, Y Hayashi, T Sano; citation_volume=54; citation_issue=3; citation_publication_date=2004; citation_pages=145-150; citation_doi=10.1111/j.1440-1827.2003.01600.x; citation_id=CR27
citation_journal_title=Expert Rev Anticancer Ther; citation_title=Role of chemotherapy in the management of soft tissue sarcomas; citation_author=D Krikelis, I Judson; citation_volume=10; citation_issue=2; citation_publication_date=2010; citation_pages=249-260; citation_doi=10.1586/era.09.176; citation_id=CR28