Clinical evaluation of a disposable amperometric magneto-genosensor for the detection and identification of Streptococcus pneumoniae

Bettazzi, 2013, Electrochemical detection of miRNA-222 by use of a magnetic bead-based bioassay, Anal. Bioanal. Chem., 405, 1025, 10.1007/s00216-012-6476-7 Blaschke, 2011, Interpreting assays for the detection of Streptococcus pneumoniae, Clin. Infect. Dis., 52, S331, 10.1093/cid/cir048 Campuzano, 2011, Development of amperometric magnetogenosensors coupled to asymmetric PCR for the specific detection of Streptococcus pneumoniae, Anal. Bioanal. Chem., 399, 2413, 10.1007/s00216-010-4645-0 Chen, 2007, Immunomagnetic separation and MS/SPR end-detection combined procedure for rapid detection of Staphylococcus aureus and protein A, Biosens. Bioelectron., 22, 1487, 10.1016/j.bios.2006.06.038 Drummond, 2003, Electrochemical DNA sensors, Nat. Biotechnol., 21, 1192, 10.1038/nbt873 Harris, 2008, Duplex real-time PCR assay for detection of Streptococcus pneumoniae in clinical samples and determination of penicillin susceptibility, J. Clin. Microbiol., 46, 2751, 10.1128/JCM.02462-07 Llull, 2006, Characteristic signatures of the lytA gene provide a basis for rapid and reliable diagnosis of Streptococcus pneumoniae infections, J. Clin. Microbiol., 44, 1250, 10.1128/JCM.44.4.1250-1256.2006 López, 2004, Recent trends on the molecular biology of pneumococcal capsules, lytic enzymes, and bacteriophage, FEMS Microbiol. Rev., 28, 553, 10.1016/j.femsre.2004.05.002 Lucarelli, 2008, Electrochemical and piezoelectric DNA biosensors for hybridisation detection, Anal. Chim. Acta., 609, 139, 10.1016/j.aca.2007.12.035 Lund, 1978, Laboratory diagnosis, serology and epidemiology of Streptococcus pneumoniae, 241, 10.1016/S0580-9517(08)70365-9 Marriott, 2008, Pneumolysin: a double-edged sword during the host–pathogen interaction, Curr. Mol. Med., 8, 497, 10.2174/156652408785747924 Obregón, 2002, Molecular peculiarities of the lytA gene isolated from clinical pneumococcal strains that are bile insoluble, J. Clin. Microbiol., 40, 2545, 10.1128/JCM.40.7.2545-2554.2002 Pedrero, 2012, Magnetic beads-based electrochemical sensors applied to the detection and quantification of bioterrorism/biohazard agents, Electroanalysis, 24, 470, 10.1002/elan.201100528 Perkins, 2006, The inconsistency of “optimal” cutpoints obtained using two criteria based on the receiver operating characteristic curve, Am. J. Epidemiol., 163, 670, 10.1093/aje/kwj063 Rouphael, 2008, A real-time polymerase chain reaction for the detection of Streptococcus pneumoniae in blood using a mouse model: a potential new “gold standard”, Diagn. Microbiol. Infect. Dis., 62, 23, 10.1016/j.diagmicrobio.2008.06.002 Sheppard, 2004, Autolysin-targeted LightCycler assay including internal process control for detection of Streptococcus pneumoniae DNA in clinical samples, J. Med. Microbiol., 53, 189, 10.1099/jmm.0.05460-0 Smith, 2009, Diagnosis of Streptococcus pneumoniae infections in adults with bacteremia and community-acquired pneumonia: clinical comparison of pneumococcal PCR and urinary antigen detection, J. Clin. Microbiol., 47, 1046, 10.1128/JCM.01480-08 Wei, 2010, DNA diagnostics: nanotechnology-enhanced electrochemical detection of nucleic acids, Pediatr. Res., 67, 458, 10.1203/PDR.0b013e3181d361c3