Classification of NPY-Expressing Neocortical Interneurons

Journal of Neuroscience - Tập 29 Số 11 - Trang 3642-3659 - 2009
Anastassios Karagiannis1,2, Thierry Gallopin1, Csaba Dávid3, Demian Battaglia4,5, H Geoffroy1, Jean Rossier1, Elizabeth M. C. Hillman6, Kathleen S. Rockland3, Bruno Cauli1,2
1NDC - Neurobiologie et diversité cellulaire (ESPCI, 10 Rue Vauquelin 75231 PARIS CEDEX 05 - bat.E / F 4ème étage - France)
2NPA - Neurobiologie des processus adaptatifs (bat. B , 4è, 5è, 6è étages 9 Quai Saint-Bernard - BP case1 75252 PARIS CEDEX 05 - France)
3Institute of Anatomy and Cell Biology (Department of Neuroanatomy, D-79001 Freiburg, Germany. - Germany)
4Bernstein CenterComputational Neuroscience, (D-37073 Göttingen, - Germany)
5NPSM - Neurophysique et physiologie du système moteur (45 Rue des Saints-Pères 75270 PARIS CEDEX 06 - France)
6Department of Biomedical Engineering, (351L Engineering Terrace, 1210 Amsterdam Avenue, New York, NY 10027, USA - United States)

Tóm tắt

Neuropeptide Y (NPY) is an abundant neuropeptide of the neocortex involved in numerous physiological and pathological processes. Because of the large electrophysiological, molecular, and morphological diversity of NPY-expressing neurons their precise identity remains unclear. To define distinct populations of NPY neurons we characterized, in acute slices of rat barrel cortex, 200 cortical neurons of layers I–IV by means of whole-cell patch-clamp recordings, biocytin labeling, and single-cell reverse transcriptase-PCR designed to probe for the expression of well established molecular markers for cortical neurons. To classify reliably cortical NPY neurons, we used and compared different unsupervised clustering algorithms based on laminar location and electrophysiological and molecular properties. These classification schemes confirmed that NPY neurons are nearly exclusively GABAergic and consistently disclosed three main types of NPY-expressing interneurons. (1) Neurogliaform-like neurons exhibiting a dense axonal arbor, were the most frequent and superficial, and substantially expressed the neuronal isoform of nitric oxide synthase. (2) Martinotti-like cells characterized by an ascending axon ramifying in layer I coexpressed somatostatin and were the most excitable type. (3) Among fast-spiking and parvalbumin-positive basket cells, NPY expression was correlated with pronounced spike latency. By clarifying the diversity of cortical NPY neurons, this study establishes a basis for future investigations aiming at elucidating their physiological roles.

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Journal of Neuroscience

Tập 29 Số 11

3642-3659

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