Chromosomal mapping and gene disruption of the ADHIII gene in Aspergillus nidulans
Tóm tắt
There are at least three alcohol dehydrogenases in Aspergillus nidulans. ADHIII has no obvious physiological function. We describe here the cloning of the ADHIII gene (alcC), its mapping on linkage group VII by “reverse genetics”, and the properties of multicopy transformants tested for their ability to grow on a range of alcohols (butan-1-ol being the best substrate tested for growth). We were unable to detect any obvious alteration in phenotype of a strain carrying a disrupted copy of the ADHIII gene.
Tài liệu tham khảo
Arst HN Jr, Scazzocchio C (1985) Formal genetics and molecular biology of the control expression in Aspergillus nidulans. In: Bennet JW, Lasure LL (eds) Gene manipulations in fungi. Academic Press, New York, pp 309–343
Bailey C, Arst HN Jr (1975) Eur J Biochem 51:573–577
Creaser EH, Porter RL, Britt KA, Pateman JA, Doy CH (1984) Biochem J 225:449–454
Clutterbuck AJ (1974) In: King RC (ed) Handbook of genetics. Plenum Press, New York
Clutterbuck AJ (1987) In: Genetic maps. Cold Spring Harbor Laboratory. Cold Spring Harbor, New York
Cove DJ (1966) Biochim Biophys Acta 113:51–56
Fidel F, Doonan JH, Morris MR (1988) Gene 70:283–293
Goa J (1953) Scand J Clin Lab Invest 5:218–222
Gurr SJ, Unkles SE, Kinghorn JR (1987) The structure and organisation of genes of filamentous fungi. In: Kinghorn JR (ed) Gene structure in eukaryotic microbes. SGM Special Publications 22. IRL Press, Oxford
Gwynne DI, Buxton FP, Sibley S, Davies RW, Lockington RA, Scazzocchio C, Sealy-Lewis HM (1987) Gene 51:205–216
Hynes MJ (1972) J Bacteriol 111:717–222
Hynes MJ (1978a) Mol Gen Genet 161:59–65
Hynes MJ (1978b) Mol Gen Genet 166:31–36
Hynes MJ, Corrick CM, King JA (1983) Mol Cell Biol 3:1430–1439
Kelly J, Hynes MJ (1987) Curr Genet 12:21–31
Lockington RA, Sealy-Lewis HM, Scazzocchio C, Davies RW (1985) Gene 33:137–149
Lockington RA, Scazzocchio C, Sequeval D, Mathieu M, Felenbok B (1987) Mol Microbiol 1 (3):275–281
Maniatis T, Frisch EF, Sambrook J (1982) Molecular cloning. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
McKnight GL, Kato H, Upshall A, Parker MD, Saari G, O'Hara PJ (1985) EMBO J 4:2093–2099
Pateman JA, Doy CH, Olsen JE, Norris U, Creaser EH, Hynes MJ (1983) Proc R Soc Lond B 217:243–264
Pickett M, Gwynne DI, Buxton FP, Elliott R, Davies RW, Lockington RA, Scazzocchio C, Sealy-Lewis HM (1987) Gene 51:217–222
Pontecorvo G, Roper JA, Hemmons LM, MacDonald KD, Buxton AWJ (1953) Adv Genet 5:141–238
Raibaud O, Schwartz M (1984) Annu Rev Genet 18:173–206
Sealy-Lewis HM, Lockington RA (1984) Curr Genet 8:253–259
Tilburn J, Scazzocchio C, Taylor GG, Zabicky-Zissman JH, Lockington RA, Davies RW (1983) Gene 26:205–221
Yelton MM, Hamer JE, Timberlake WE (1984) Proc Natl Acad Sci USA 81:1470–1474