Chemical Modification of the Mitochodrial <i>bc<sub>1</sub></i> by <i>N,N′</i> ‐Dicyclohexylcarbodiimide Inhibits Proton Translocation

FEBS Journal - Tập 132 Số 3 - Trang 595-601 - 1983
Brendan D. Price1, Martin D. Brand1
1Department of Biochemistry, University of Cambridge, Tennis, Court Road, Cambridge, Great Britain, CB2 1QW

Tóm tắt

We report here that N,N/i′‐dicyclohexylcarbodiimide (DCCD) decreases the H2e stoichiometry of the mytochrome bc1 complex from 308 ± 0.2 (10) to 201 ± 0.1 (8) but has only a minimal effect on the H2e ratio of cytochrome oxidase under the relatively mild condition used. The veffect on the bc1 complex connot be explained by uncoupling, by inhibition of electron transport or by selective mitochondrial damage.We conclude that DCCD is an inhibitor of proton translocation within ther bc1 complex. There are three possible explanation of this effect: (a) DCCD could alter the pathway of electron flow, (b) DCCD could prevent one of the proton translocation reations but not electrton transport, (c) DCCD could prevent the couduction of the translocated proton to the external phase.

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FEBS Journal

Tập 132 Số 3

595-601

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