Characterization of theStreptomyces coelicolor A3(2)wblE gene, encoding a homologue of the sporulation transcription factor
Tóm tắt
The gene corresponding to the recently identifiedwhiB-paralogous genewblE inS. coelicolor was found after sequencing the downstream region of the stress-response sporulation-specific σ-factor gene,sigH, inS. coelicolor A3(2). Sequence analysis has revealed an ORF exhibiting high similarity to sporulation transcription factors WhiB and WhiD. A stable null mutant of thewblE gene was obtained by integrative transformation,via double cross-over. Disruption of theS. coelicolor wblE gene appeared to have no obvious effect on growth, morphology, differentiation, and production of the pigmented antibiotics actinorhodin and undecylprodigiosin. Expression of thewblE gene was investigated during differentiation by S1 nuclease mapping, using RNA prepared fromS. coelicolor A3(2) and its isogenicsigF andsigH mutants grown to various developmental stages. A single promoter was identified upstream of thewblE coding region. ThewblEp promoter was induced at the beginning of aerial mycelium formation and its activity decreased later in differentiation. No differences in expression of thewblEp promoter were detected inS. coelicolor A3(2) mutants insigF andsigH genes for sporulation-specific σ factors. Sequence of thewblEp promoter showed partial similarity to the consensus sequence of the extracytoplasmic σ factors.
Tài liệu tham khảo
Ausubel F.M., Brent R., Kingston R.E., Moore D.O., Seidman J.S., Smith J.A., Struhl K.:Current Protocols in Molecular Biology. John Wiley & Sons, New York 1995.
Chater K.F.: Taking a genetic scalpel to theStreptomyces colony.Microbiology 144, 1465–1478 (1998).
Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C., Harris D., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III,Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R., Devlin K., Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L., Oliver K., Osborne J., Quail M.A., Rajandream M.-A., Rogers J., Rutter S., Seeger K., Skelton J., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G.: Deciphering the biology ofMycobacterium tuberculosis from the complete genome sequence.Nature 393, 537–544 (1998).
Davis N.K., Chater K.F.: TheStreptomyces coelicolor whiB gene encodes a small transcription factor-like protein dispensable for growth but essential for sporutation.Mol.Gen.Genet. 232, 351–358 (1992).
Gomez J.E., Bishai W.R.:whmD is an essential mycobacterial gene required for proper septation and cell division.Proc.Nat.Acad.Sci.USA 97, 8554–8559 (2000).
Helmann J.D.: The extracytoplasmic function (ECF) σ factors.Adv.Microbial.Physiol. 46, 48–110 (2002).
Kelemen G.H., Brown P., Kormanec J., Potúčková L., Chater K.F., Buttner M.: The position of the σ-factor genes,whiG andsigF, in the hierarchy controlling the development of spore chains in the aerial hyphae ofStreptomyces coelicolor A3(2).Mol.Microbiol. 21, 593–603 (1996).
Kelemen G.H., Brian P., Flardh K., Chamberlin L., Chater K.F., Buttner J.: Developmental regulation of transcription ofwhiE, a locus specifying the polyketide spore pigment inStreptomyces coelicolor A3(2).J.Bacteriol. 180, 2515–2521 (1998).
Kelemen G.H., Plaskitt K.H., Lewis C.G., Findlay K.C., Butiner J.: Deletion of DNA lying close to theglkA locus induces ectopic sporulation inStreptomyces coelicolor A3(2).Mol.Microbiol. 17, 221–230 (2001).
Kieser T., Bibb M.J., Buttner M.J., Chater K.F., Hopwood D.A.:Practical Streptomyces Genetics. The John Innes Foundation, Norwich (UK) 2000.
Kormanec J.: Analyzing the developmental expression of σ factors with S1 nuclease mapping, pp. 481–494 in C.H. Chein (Ed.):Nuclease Methods and Protocols. Methods in Molecular Biology. Humana Press, Totowa (USA) 2001.
Kormanec J., Řežuchová B., Farkašovský M.: Optimization ofStreptomyces aureofaciens transformation and disruption of thehrdA gene encoding a homologue of the principal σ factor.J.Gen.Microbiol. 139, 2525–2529 (1993).
Kormanec J., Ševčiková B., Sprušanský O., Benada O., Kofroňová O., Novaková R., Řežuchová B., Potúčková L., Homerová D.: TheStreptomyces aureofaciens homologue of thewhiB gene is essential for sporulation and its expression correlates with the developmental stage.Folia Microbiol. 43, 605–612 (1998).
Kormanec J., Ševčíková B., Halgašová N., Knirschová R., Řežuchová B.: Identification and transcription characterization of the gene encoding the stress-response σ factor σH inStreptomyces coelicolor A3(2).FEMS Microbiol.Lett. 189, 31–38 (2000).
Kormanec J., Homerova D., Ševčíková B., Řežuchová B.: A method for isolation of small DNA fragments from agarose and polyacrylamide gels.Anal.Biochem. 293, 138–139 (2001).
Macneil D.J., Gewain K.M., Ruby C.L., Dezeny G., Gibbons P.H., Macneil T.: Analysis ofStreptomyces avermitilis genes required for avermectin biosynthesis utilizing a novel integration vector.Gene 111, 61–68 (1992).
Maxam A.M., Gilbert W.: Sequencing end-labeled DNA with base specific chemical cleavages.Methods Enzymol. 65, 449–560 (1980).
Molle V., Palframan W.J., Findlay K.C., Buttner M.J.: WhiD and WhiB, homologous proteins required for different stages of sporulation inStreptomyces coelicolor A3(2).J.Bacteriol. 182, 1284–1295 (2000).
Paget M.S.B., Hong H.-J., Bibb M.J., Butiner M.J.: The ECF σ factors ofStreptomyces coelicolor A3(2), pp. 105–125 in D.A. Hodgson, C.M. Thomas (Eds):Signals, Switches, Regulons and Cascades: Control of Bacterial Gene Expression. Cambridge University Press, Cambridge (UK) 2002.
Potučková L., Kelemen G.H., Findlay K.C., Lonetto M.A., Buttner M.J., Kormanec J.: A new RNA polymerase σ factor, σF, is required for the late stages of morphological differentiation inStreptomyces spp.Mol.Microbiol. 17, 37–48 (1995).
Seibert V., Kourbatova E.M., Golovleva L.A., Schlomann M.: Characterization of the maleylacetate reductase MacA ofRhodococcus opacus 1CP and evidence for the presence of an isofunctional enzyme.J.Bacteriol. 180, 3503–3508 (1998).
Ševčíková B., Benada O., Kofroňová O., Kormanec J.: Stress response σ factor σH is essential for morphological differentiation ofStreptomyces coelicolor A3(2).Arch.Microbiol. 177, 98–106 (2001).
Smith D.R., Richterich P., Rubenfield M., Rice P.W., Butler C., Lee H.-M., Kirst S., Gundersen K., Abendschan K., Xu Q., Chung M., Deloughery C., Aldredge T., Maher J., Lundstrom R., Tulig C., Falls K., Imrich J., Torrey D., Engelstein M., Breton G., Madan D., Nietupski R., Seitz B., Connelly S., McDougall S., Safer H., Gibson R., Doucette-Stamm L., Eiglmeier K., Bergh S., Cole S.T., Robison K., Richterich L., Johnson J., Church G.M., Mao J.: Multiplex sequencing of 1.5 Mb of theMycobacterium leprae genome.Genome Res. 7, 802–819 (1997).
Soliveri J., Brown K.L., Buttner M.J., Chater K.F.: Two promoters for thewhiB sporulation gene ofStreptomyces coelicolor A3(2) and their activities in relation to development.J.Bacteriol. 174, 6215–6220 (1992).
Soliveri J., Granozzi C., Plaskitt K.A., Chater K.F.: Functional and evolutionary implications of a survey of various actinomycetes for homologues of twoStreptomyces coelicolor sporulation genes.J.Gen.Microbiol. 139, 2569–2578 (1993).
Soliveri J., Gomez J., Bishai W.R., Chater K.F.: Multiple paralogous genes related to theStreptomyces coelicolor developmental regulatory genewhiB are present inStreptomyces and other actinomycetes.Microbiology 146, 333–343 (2000).
Steyn A.J.C., Collins D.M., Hondalus M.K., Jacobs W.R., Kawakami R.P., Bloom B.R.:Mycobacterium tuberculosis WhiB3 interacts with RpoV to affect host survival but is dispensable forin vivo growth.Proc.Nat.Aced.Sci.USA 99, 3147–3152 (2001).
van Wezel G.P., Bibb M.J.: A novel plasmid vector that uses glucose kinase gene (glkA) for the positive selection of stable gene disruptants inStreptomyces.Gene 182, 229–230 (1996).