Characterization of the multiple resistance traits of somatic hybrids between Solanum cardiophyllum Lindl. and two commercial potato cultivars

Plant Cell Reports - Tập 29 - Trang 1187-1201 - 2010
Ramona Thieme1, Elena Rakosy-Tican2, Marion Nachtigall1, Jörg Schubert3, Thilo Hammann1, Olga Antonova4, Tatjana Gavrilenko4, Udo Heimbach5, Thomas Thieme6
1Julius Kühn Institute, Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Agricultural Crops, Quedlinburg, Germany
2Babeş-Bolyai University, Cluj-Napoca, Romania
3Julius Kühn Institute, Federal Research Centre for Cultivated Plants, Institute for Biosafety of Genetically Modified Plants, Quedlinburg, Germany
4NI Vavilov Institute of Plant Industry, St. Petersburg, Russia
5Julius Kühn Institute, Federal Research Centre for Cultivated Plants, Institute for Plant Protection in Field Crops and Grassland, Braunschweig, Germany
6BTL Bio-Test Lab GmbH Sagerheide, Sagerheide, Germany

Tóm tắt

Interspecific somatic hybrids between commercial cultivars of potato Solanum tuberosum L. Agave and Delikat and the wild diploid species Solanum cardiophyllum Lindl. (cph) were produced by protoplast electrofusion. The hybrid nature of the regenerated plants was confirmed by flow cytometry, simple sequence repeat (SSR), amplified fragment length polymorphism (AFLP), microsatellite-anchored fragment length polymorphism (MFLP) markers and morphological analysis. Somatic hybrids were assessed for their resistance to Colorado potato beetle (CPB) using a laboratory bioassay, to Potato virus Y (PVY) by mechanical inoculation and field trials, and foliage blight in a greenhouse and by field trials. Twenty-four and 26 somatic hybrids of cph + cv. Agave or cph + cv. Delikat, respectively, showed no symptoms of infection with PVY, of which 3 and 12, respectively, were also resistant to foliage blight. One hybrid of cph + Agave performed best in CPB and PVY resistance tests. Of the somatic hybrids that were evaluated for their morphology and tuber yield in the field for 3 years, four did not differ significantly in tuber yield from the parental and standard cultivars. Progeny of hybrids was obtained by pollinating them with pollen from a cultivar, selfing or cross-pollination. The results confirm that protoplast electrofusion can be used to transfer the CPB, PVY and late blight resistance of cph into somatic hybrids. These resistant somatic hybrids can be used in pre-breeding studies, molecular characterization and for increasing the genetic diversity available for potato breeding by marker-assisted combinatorial introgression into the potato gene pool.

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Plant Cell Reports

Tập 29

1187-1201

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