Characterization of the intestinal mucosal proteome in cats with inflammatory bowel disease and alimentary small cell lymphoma

Journal of Veterinary Internal Medicine - Tập 35 Số 1 - Trang 179-189 - 2021
Sina Marsilio1,2, Floris Dröes2, Lawrence J. Dangott3, Betty Chow4,5, Steve L. Hill6,5, Mark R. Ackermann7, J Scott Estep8, Jonathan A. Lidbury2, Jan S. Suchodolski2, Jörg M. Steiner2
1Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA, USA
2Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences Texas A&M College of Veterinary Medicine & Biomedical Sciences, Texas A&M University College Station Texas USA
3Protein Chemistry Laboratory, Department of Biochemistry & Biophysics Texas A&M University College Station Texas USA
4VCA Animal Specialty & Emergency Center Los Angeles California USA
5Veterinary Specialty Hospital, San Diego, California, USA
6Flagstaff Veterinary Internal Medicine Consulting, Flagstaff, Arizona, USA
7Oregon Veterinary Diagnostic Laboratory, Carlson College of Veterinary Medicine, Oregon State University, Corvallis, Oregon, USA
8Texas Veterinary Pathology, LLC, San Antonio, Texas, USA

Tóm tắt

AbstractBackgroundCurrent tests for diagnosis and differentiation of lymphoplasmacytic enteritis (LPE) and small cell lymphoma (SCL) in cats are expensive, invasive, and lack specificity. The identification of less invasive, more reliable biomarkers would facilitate diagnosis.ObjectivesTo characterize the mucosal proteome in endoscopically obtained, small intestinal tissue biopsy specimens. We hypothesized that differentially expressed proteins could be identified and serve as biomarker candidates for the differentiation of LPE and SCL in cats.AnimalsSix healthy control cats, 6 cats with LPE, and 8 cats with SCL.MethodsThe mucosal proteome was analyzed using 2‐dimensional fluorescence difference gel electrophoresis (2D DIGE) and nanoflow liquid chromatography tandem mass spectrometry. For 5 proteins, results were verified by Western blot analysis.ResultsA total of 2349 spots were identified, of which 9 were differentially expressed with a ≥2‐fold change between healthy cats and cats with LPE and SCL (.01 < P < .001). Eight of these 9 spots were also differentially expressed between cats with LPE and cats with SCL (P .001 < P < .04). However, Western blot analysis for malate dehydrogenase‐1, malate dehydrogenase‐2, apolipoprotein, annexin IV, and annexin V did not confirm significant differential protein expression for any of the 5 proteins assessed.Conclusions and Clinical ImportanceTwo‐D DIGE did not identify potential biomarker candidates in the intestinal mucosa of cats with LPE and SCL. Future studies should focus on different techniques to identify biomarker candidates for cats with chronic enteropathies (CE).

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Tài liệu tham khảo

10.1111/j.1939-1676.2003.tb02450.x

10.1016/j.cvsm.2011.02.003

10.1177/1098612X12451548

10.1111/jsap.12494

10.1177/0300985811404712

10.1177/0300985810389479

10.1186/s12917-018-1635-5

10.1016/j.vetimm.2005.02.014

10.2478/raon-2013-0072

10.1111/jvim.15455

10.1111/jvim.15742

10.1067/mcp.2001.113989

10.1002/pmic.201700448

10.1002/elps.11501601185

Clark DP, 2013, Proteomics: The Global Analysis of Proteins

10.1038/nprot.2006.256

10.1016/B978-0-12-405914-6.00006-8

10.1016/j.jcpa.2008.01.001

10.1111/j.1939-1676.2009.0443.x

Keller SM, 2012, Clonality testing in veterinary medicine: a review with diagnostic guidelines, Vet Pathol, 49, 658

10.1038/leu.2012.246

10.1038/sj.leu.2403202

10.1016/0003-2697(84)90782-6

10.1016/0003-2697(76)90527-3

Tonge R, 2001, Validation and development of fluorescence two‐dimensional differential gel electrophoresis proteomics, Dent Tech, 1, 377

10.1002/pmic.200390006

10.1111/j.1462-5822.2007.00901.x

10.1007/s12975-010-0048-y

10.1586/14789450.5.2.283

10.1002/ibd.20652

10.1002/pmic.200300635

10.7150/jca.27560

10.1002/pmic.200800298

10.1016/B978-0-12-800094-6.00004-2

User manual vertical electrophoresis Ettan Daltsix Electrophoresis System Amersham Bioscience. San Francisco CA USA.

10.1038/labinvest.2009.87

Chahed K, 2005, Expression of fibrinogen E‐fragment and fibrin E‐fragment is inhibited in the human infiltrating ductal carcinoma of the breast: the two‐dimensional electrophoresis and MALDI‐TOF‐mass spectrometry analyses, Int J Oncol, 27, 1425

10.1002/pmic.200300606

10.1371/journal.pone.0124864

Chen GA, 2002, Proteomic analysis of lung adenocarcinoma: identification of a highly expressed set of proteins in tumors, Clin Cancer Res, 8, 2298

10.1021/ac011159c

10.1074/mcp.M113.028027

10.1158/1078-0432.CCR-03-0321

10.4061/2009/239204

10.1586/14789450.2014.939635

10.1155/2019/5214821

10.1002/pmic.200400941

10.1177/0300985816638724

10.1111/j.1939-1676.2009.0457.x

10.1111/jvim.15471

Force USPST.Section 6. Methods for Arriving at a Recommendation2017.

NCCN.NCCN clinical practice guidelines in oncology (NCCN guidelines®) esophageal and Esophagogastric junction cancers. Version 1.20142014.

10.7326/M18-0694

10.1136/bmjopen-2017-018448

10.1136/bmj.e3502

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Journal of Veterinary Internal Medicine

Tập 35 Số 1

179-189

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