Characterization of immunomodulatory, anticancer and antioxidant properties of an extracellular polymer produced by Enterococcus sp. in vegetable waste medium

Environmental Sustainability - Tập 4 - Trang 419-428 - 2021
Vivek Sharma1, Moushumi Ghosh2
1Department of Life and Basic Sciences, Jaipur National University, Jaipur, India
2Department of Biotechnology, Thapar Institute of Engineering and Technology, Patiala, India

Tóm tắt

This study attempted to evaluate the bioactive properties and characteristics of an extracellular polymer produced by Enterococcus sp. grown in a newly developed vegetable waste media. The vegetable waste medium formulated from corn, garlic, and onion flakes enabled enhanced production of the biopolymer in comparison to other commercially available media. The biopolymer was purified and characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), antioxidant ability by hydroxyl, superoxide and di (2, 4, 6 trinitrophenyl) iminoazanium (DPPH) radical scavenging activity. Immunomodulatory and anticancer activities were evaluated by RAW 264.7 and HeLa, respectively. Human Umbilical Vein Endothelial Cells (HUVEC) cell lines were used to determine toxicity to the endothelial system. The molecular mass of the biopolymer was ~ 2 × 105 kDa and it consisted of carbohydrates, uronic acids, hexosamines and proteins. Significant (p < 0.05) production of nitric oxide, tumor necrosis factor (TNF-α), Interleukin-6 (IL-6), IL-ß and antioxidant activity were observed at 100 µg/mL biopolymer concentration. FTIR and SEM analysis revealed a biocompatible, highly porous biopolymer structure with technological suitability. The antioxidant, immunomodulatory and antitumor properties of the Enterococcus sp. biopolymer are likely to have biomedical implications and offer a sustainable approach in terms of de-scaling the production economics while effectively valorizing agro-wastes.

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