Characterization of galactose-dependent promoters from an oleaginous fungus Mortierella alpina 1S-4

Takuo Okuda1, Akinori Ando1, Eiji Sakuradani1, Hiroshi Kikukawa1, Noriaki Kamada2, Misa Ochiai2, Jun Shima3, Jun Ogawa1
1Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto, 606-8052, Japan
2Research Institute, Suntory Global Innovation Center Ltd., 1-1-1 Wakayamadai, Shimamoto-cho, Mishima-gun, Osaka 618-8503, Japan
3Research Division of Microbial Sciences, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto, 606-8052, Japan

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Amann E, Brosius J, Ptashne M (1983) Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli. Gene 25:167–178

Ando A, Sakuradani E, Horinaka K, Ogawa J, Shimizu S (2009a) Transformation of an oleaginous zygomycete Mortierella alpina 1S-4 with the carboxin resistance gene conferred by mutation of the iron-sulfur subunit of succinate dehydrogenase. Curr Genet 55:349–356

Ando A, Sumida Y, Negoro H, Suroto DA, Ogawa J, Sakuradani E, Shimizu S (2009b) Establishment of Agrobacterium tumefaciens-mediated transformation of an oleaginous fungus, Mortierella alpina 1S-4, and its application for eicosapentaenoic acid producer breeding. Appl Environ Microbiol 75:5529–5535

Archer DB, Jeenes DJ, Mackenzie DA (1994) Strategies for improving heterologous protein production from filamentous fungi. Antonie Van Leeuwenhoek 65:245–250

Arima K, Yu J, Iwasaki S (1970) Milk-clotting enzyme from Mucor pusillus var. Lindt. Methods Enzymol 19:446–459

Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72:248–254

Cereghino JL, Cregg JM (2000) Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev 24:45–66

Chien CT, Bartel PL, Sternglanz R, Fields S (1991) The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest. Proc Natl Acad Sci USA 88:9578–9582

Gancedo JM (1998) Yeast carbon catabolite repression. Microbiol Mol Biol Rev 62:334–361

Gill I, Valivety R (1997) Polyunsaturated fatty acids, part 1: occurrence, biological activities and applications. Trends Biotechnol 15:401–409

Giniger E, Ptashne M (1988) Cooperative DNA binding of the yeast transcriptional activator GAL4. Proc Natl Acad Sci USA 85:382–386

Giniger E, Varnum SM, Ptashne M (1985) Specific DNA binding of GAL4, a positive regulatory protein of yeast. Cell 40:767–774

Götz F (1995) A promoter-screening plasmid and xylose-inducible, glucose-repressible expression vectors for Staphylococcus carnosus. Gene 158:91–96

Griggs DW, Johnston M (1991) Regulated expression of the GAL4 activator gene in yeast provides a sensitive genetic switch for glucose repression. Proc Natl Acad Sci USA 88:8597–8601

Hansson L, Blackberg L, Edlund M, Lundberg L, Stromqvist M, Hernell O (1993) Recombinant human milk bile salt-stimulated lipase. Catalytic activity is retained in the absence of glycosylation and the unique proline-rich repeats. J Biol Chem 268:26692–26698

Ichishima E, Taya N, Ikeguchi M, Chiba Y, Nakamura M, Kawabata C, Inoue T, Takahashi K, Minetoki T, Ozeki K, Kumagai C, Gomi K, Yoshida T, Nakajima T (1999) Molecular and enzymic properties of recombinant 1,2-α-mannosidase from Aspergillus saitoi overexpressed in Aspergillus oryzae cells. Biochem J 339:589–597

Jareonkitmongkol S, Kawashima H, Shirasaka N, Shimizu S, Yamada H (1992) Production of dihomo-γ-linolenic acid by a Δ5-desaturase-defective mutant of Mortierella alpina 1S-4. Appl Environ Microbiol 58:2196–2200

Jareonkitmongkol S, Shimizu S, Yamada H (1993) Production of an eicosapentaenoic acid-containing oil by a Δ12 desaturase-defective mutant of Mortierella alpina 1S-4. J Am Oil Chem Soc 70:119–123

Jefferson RA, Burgess SM, Hirsh D (1986) β-Glucuronidase from Escherichia coli as a gene-fusion marker. Proc Natl Acad Sci USA 83:8447–8451

Johnston M (1987) A model fungal gene regulatory mechanism: the GAL genes of Saccharomyces cerevisiae. Microbiol Rev 51:458–476

Johnston M, Davis RW (1984) Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae. Mol Cell Biol 4:1440–1448

Johnston M, Flick J, Spexton T (1994) Multiple mechanisms provide rapid and stringent glucose repression of GAL gene expression in Saccharomyces cerevisiae. Mol Cell Biol 14:3834–3841

Kaieda M, Samukawa T, Matsumoto T, Ban K, Kondo A, Shimada Y, Noda H, Nomoto F, Ohtsuka K, Izumoto E, Fukuda H (1999) Biodiesel fuel production from plant oil catalyzed by Rhizopus oryzae lipase in a water-containing system without an organic solvent. J Biosci Bioeng 88:627–631

Kawashima H, Kamada N, Sakuradani E, Jareonkitmongkol S, Akimoto K, Shimizu S (1997) Production of 8,11,14,17-cis-eicosatetraenoic acid by Δ5 desaturase-defective mutants of an arachidonic acid-producing fungus, Mortierella alpina. J Am Oil Chem Soc 74:455–459

MacKenzie DA, Jeenes DJ, Belshaw NJ, Archer DB (1993) Regulation of secreted protein production by filamentous fungi: recent developments and perspectives. J Gen Microbiol 139:2295–2307

Meisenzahl AC, Shapiro L, Jenal U (1997) Isolation and characterization of a xylose-dependent promoter from Caulobacter crescentus. J Bacteriol 179:592–600

Millati R, Edebo L, Taherzadeh MJ (2005) Performance of Rhizopus, Rhizomucor, and Mucor in ethanol production from glucose, xylose, and wood hydrolyzates. Enzyme Microb Technol 36:294–300

Punt PJ, Van Biezen N, Conesa A, Albers A, Mangnus J, Van Den Hondel C (2002) Filamentous fungi as cell factories for heterologous protein production. Trends Biotechnol 20:200–206

Rodrigues RC, Fernandez-Lafuente R (2010) Lipase from Rhizomucor miehei as an industrial biocatalyst in chemical process. J Mol Catal B Enzym 64:1–22

Sakuradani E, Kobayashi M, Shimizu S (1999) Δ9-Fatty acid desaturase from arachidonic acid-producing fungus. Unique gene sequence and its heterologous expression in a fungus, Aspergillus. Eur J Biochem 260:208–216

Sakuradani E, Ando A, Shimizu S, Ogawa J (2013) Metabolic engineering for the production of polyunsaturated fatty acids by oleaginous fungus Mortierella alpina 1S-4. J Biosci Bioeng 116:417–422

Shen WJ, Forde BG (1989) Efficient transformation of Agrobacterium spp. by high voltage electroporation. Nucleic Acids Res 17:8385

Shimizu S, Ogawa J, Kataoka M, Kobayashi M (1997) Screening of novel microbial enzymes for the production of biologically and chemically useful compounds. Adv Biochem Eng 58:45–87

Sumi A, Okuyama K, Kobayashi K, Ohtani W, Ohmura T, Yokoyama K (1999) Purification of recombinant human serum albumin. Efficient purification using STREAMLINE. Bioseparation 8:195–200

Synowiecki J, Al-Khateeb NA (2003) Production, properties, and some new applications of chitin and its derivatives. Crit Rev Food Sci Nutr 43:145–171

Takeno S, Sakuradani E, Murata S, Inohara-Ochiai M, Kawashima H, Ashikari T, Shimizu S (2004a) Cloning and sequencing of the ura3 and ura5 genes, and isolation and characterization of uracil auxotrophs of the fungus Mortierella alpina 1S-4. Biosci Biotechnol Biochem 68:277–285

Takeno S, Sakuradani E, Murata S, Inohara-Ochiai M, Kawashima H, Ashikari T, Shimizu S (2004b) Establishment of an overall transformation system for an oil-producing filamentous fungus, Mortierella alpina 1S-4. Appl Microbiol Biotechnol 65:419–425

Takeno S, Sakuradani E, Tomi A, Inohara-Ochiai M, Kawashima H, Shimizu S (2005) Transformation of oil-producing fungus, Mortierella alpina 1S-4, using Zeocin, and application to arachidonic acid production. J Biosci Bioeng 100:617–622

Terpe K (2006) Overview of bacterial expression systems for heterologous protein production: from molecular and biochemical fundamentals to commercial systems. Appl Microbiol Biotechnol 72:211–222

Wickes BL, Edman JC (1995) The Cryptococcus neoformans GAL7 gene and its use as an inducible promoter. Mol Microbiol 16:1099–1109