Characterization of four defense-related genes up-regulated in root nodules of Casuarina glauca

Symbiosis - Tập 50 - Trang 27-35 - 2009
Patrícia Santos1,2,3, Ana Fortunato1,2, Inês Graça1,2, Sandra Marina Martins2, Maria Manuela Gouveia4, Florence Auguy5, Didier Bogusz5, Cândido Pereira Pinto Ricardo2,6, Katharina Pawlowski3, Ana Ribeiro1,2
1ECO-BIO/Tropical Research Institute, Oeiras, Portugal
2ITQB/UNL, Av. da República (EAN), Oeiras, Portugal
3Department of Botany, Stockholm University, Stockholm, Sweden
4Departamento de Biologia and Centro de Estudos da Macaronésia, Universidade da Madeira, Funchal, Portugal
5Unité Mixte de Recherche 1098, Institut de Recherche pour le Développement, Montpellier cedex 5, France
6ISA/UTL, Tapada da Ajuda, Lisbon, Portugal

Tóm tắt

Actinorhizal plants are capable of high rates of nitrogen fixation, due to their capacity to establish a root-nodule symbiosis with N2-fixing actinomycetes of the genus Frankia. Nodulation is an ontogenic process which requires a sequence of highly coordinated events. One of these mechanisms is the induction of defense-related events, whose precise role during nodulation is largely unknown. In order to contribute to the clarification of the involvement of defense-related genes during actinorhizal root-nodule symbiosis, we have analysed the differential expression of several genes with putative defense-related functions in Casuarina glauca nodules versus non-inoculated roots. Four genes encoding a chitinase (CgChi1), a glutathione S-transferase (CgGst), a hairpin-inducible protein (CgHin1) and a peroxidase (CgPox4) were found to be up-regulated in mature nodules compared to roots. In order to find out to which extend were the encoded proteins involved in nodule protection, development or both, gene regulation studies in response to SA and wounding as well as phylogenetic analysis of the protein sequences were performed. These were further characterized through expression studies after SA-treatment and wounding, and by phylogenetic analysis. We suggest that CgChi1 and CgGst are involved in defense or microsymbiont control and CgPox4 is involved in nodule development. For CgHin1 the question “defense, development or both” remains open.

Tài liệu tham khảo

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