Characterization of Ultra‐Thin Temperature‐Responsive Polymer Layer and Its Polymer Thickness Dependency on Cell Attachment/Detachment Properties

Macromolecular Bioscience - Tập 10 Số 10 - Trang 1117-1129 - 2010
Kenzo Fukumori1, Yoshikatsu Akiyama2, Yoshikazu Kumashiro2, Jun Kobayashi2, Masayuki Yamato2, Kiyotaka Sakai1, Teruo Okano2
1Department of Applied Chemistry, Waseda University, 3-4-1 Ohkubo, Shinjuku, Tokyo 169-8555, Japan
2Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, TWIns, 8-1 Kawadacho, Shinjuku, Tokyo 162-8666, Japan

Tóm tắt

AbstractUltra thin poly(N‐isopropylacrylamide) (PIPAAm) modified glass coverslips (PIAPAm‐CS) using electron beam irradiation exhibited a clear relationship between the polymer thickness and thermal cell adhesion/detachment behavior. The polymer thickness dependency and the characteristic of ultra thin PIPAAm layer, has been illustrated in terms of the molecular motion of the modified PIPAAm chains. PIPAAm‐CSs surfaces with various area‐polymer densities and thicknesses were characterized by AFM and protein adsorption assay. The newly obtained results gave a further insight into the illustration. Finally, the future application of intelligent surfaces was discussed for fabricating tissue and organ.magnified image

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Tài liệu tham khảo

10.1080/10601326808051910

10.1002/polb.1990.090280609

10.1021/ac950359j

10.1016/j.chroma.2006.01.068

10.1002/pola.22868

10.1098/rsif.2008.0499.focus

10.1002/adma.200402068

10.1093/clinchem/46.9.1478

10.1002/marc.1990.030111109

10.1002/jbm.820271005

10.1016/0142-9612(95)93257-E

10.1002/(SICI)1097-4636(200008)51:2<216::AID-JBM10>3.0.CO;2-K

10.1002/1097-4636(200101)54:1<37::AID-JBM5>3.0.CO;2-7

10.1016/S0142-9612(00)00270-2

10.1089/10763270152436517

10.1016/S0142-9612(01)00225-3

10.1097/01.TP.0000110320.45678.30

10.1056/NEJMoa040455

10.1163/156856298X00424

10.1002/(SICI)1097-4636(19990615)45:4<355::AID-JBM10>3.0.CO;2-7

10.1002/jbm.10228

10.1089/ten.2006.12.499

10.1096/fj.05-4715fje

10.1002/jbm.1284

10.1161/hh0302.105722

10.1016/S0142-9612(03)00110-8

10.1016/j.jtcvs.2009.02.004

10.1021/la036139f

10.1016/j.actbio.2008.06.018

10.1021/bm0000642

10.1021/la050417o

10.1002/jbm.a.30053

10.1021/la062330o

10.1021/la048546c

10.1016/j.biomaterials.2005.09.012

10.1021/bm049675a

10.1016/j.actbio.2007.09.002

10.1021/la802556e

10.1016/j.biomaterials.2008.01.004

10.1166/jnn.2007.509

10.1021/la062770f

10.1016/j.jcis.2005.10.068

10.1016/S0022-3093(01)00378-7

10.1016/0927-7757(93)80396-V

10.1063/1.1677069

10.6028/jres.110.081

10.1016/j.bbrc.2007.07.014

10.1002/jbm.a.10423

10.1091/mbc.10.3.785

Underwood P. A., 1993, J. Cell Sci., 104, 793, 10.1242/jcs.104.3.793

10.1016/j.matbio.2005.06.008

Vroman L., 1962, Nature, 196

10.1002/jbm.820260704

10.1021/la049663a

10.1021/la050221o

10.1021/cm049562y

10.1021/la052741p

10.1021/la0531502

10.1021/la034653f

10.1021/la701461b

10.1021/la00056a017

10.1039/b101673n

10.1021/jp0473321

10.1021/ja00076a032

10.1021/la051726h

10.1002/(SICI)1097-4636(199702)34:2<189::AID-JBM8>3.0.CO;2-M

10.1016/j.biomaterials.2004.06.005

10.1021/ja044382a

10.1002/1097-4636(200104)55:1<137::AID-JBM180>3.0.CO;2-L

10.1073/pnas.101112898

10.1073/pnas.040562297

10.1038/nmeth873

10.1016/S0167-7799(03)00033-7

10.1021/ac702208d

10.1016/j.biomaterials.2005.12.023

10.1002/jbm.a.30010

10.1016/j.biomaterials.2003.08.052

10.1016/j.biomaterials.2007.08.002

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Thông tin xuất bản

Macromolecular Bioscience

Tập 10 Số 10

1117-1129

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