Tính chất của các biosurfactant ngoại bào được biểu hiện bởi một chủng Pseudomonas putida tách từ bên trong rễ của Sida hermaphrodita được trồng trong đất ô nhiễm kim loại nặng

Bernat, Przemysław1, Nesme, Joseph2,3, Paraszkiewicz, Katarzyna1, Schloter, Michael2, Płaza, Grażyna4
1Department of Industrial Microbiology and Biotechnology, Faculty of Biology and Environmental Protection, University of Łódź, Łódź, Poland
2Comparative Microbiome Analysis, Helmholtz Zentrum München, Neuherberg, Germany
3Section of Microbiology, Department of Biology, Faculty of Science, University of Copenhagen, Copenhagen, Denmark
4Institute for Ecology of Industrial Areas, Unit of Environmental Microbiology, Katowice, Poland

Tóm tắt

Pseudomonas putida E41 được phân lập từ bên trong rễ của Sida hermaphrodita (trồng trên một cánh đồng bị ô nhiễm kim loại nặng) cho thấy hoạt tính biosurfactant cao. Trong bài báo này, chúng tôi mô tả dữ liệu từ phân tích khối phổ và phân tích gen, nhằm nâng cao hiểu biết của chúng tôi về các đặc tính kiểu hình của chủng này. Dịch lỏng thu được từ nuôi cấy lỏng P. putida E41 thể hiện sự giảm mạnh độ căng bề mặt đi kèm với khả năng ổn định nhũ tương. Chúng tôi đã xác định được sự biểu hiện của lipopeptide ngoại bào, putisolvin I và II nhưng không phát hiện được rhamnolipid. Sự hiện diện của chúng được xác nhận bằng kỹ thuật khối phổ hấp phụ laser thậm chí và ion hóa (MALDI) TOF/TOF. Hơn nữa, mười phospholipid (chủ yếu là phosphatidylethanolamines PE 33:1 và PE 32:1) được bài tiết bởi các túi cũng đã được phát hiện. Ngược lại, lượng bã tế bào vi khuẩn chủ yếu được chiếm ưu thế bởi phosphatidylglycerols (PGs), mà hầu như vắng mặt trong dịch lỏng. Có vẻ như thành phần lipid ngoại bào (bài tiết ra môi trường) và lipid tế bào trong chủng này khác nhau. Kỹ thuật giải trình tự đọc dài và tái cấu trúc toàn bộ bộ gen cho phép xác định một con đường sinh tổng hợp putisolvin hoàn chỉnh. Trong bộ gen của P. putida E41 còn phát hiện tất cả các gen liên quan đến sinh tổng hợp glycerophospholipid, và chúng có thể chịu trách nhiệm cho việc sản xuất phospholipid đã được phát hiện. Tổng thể, đây là báo cáo đầu tiên mô tả sự biểu hiện của lipopeptide ngoại bào (được xác định là putisolvins) và phospholipid bởi một chủng P. putida, điều này có thể được giải thích bằng nhu cầu thích nghi với môi trường ô nhiễm nghiêm trọng.

Từ khóa

#Pseudomonas putida #biosurfactant #lipopeptides #phospholipids #môi trường ô nhiễm #kim loại nặng

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