Characterization of ERF76 promoter cloned from Populus simonii × P. nigra

Springer Science and Business Media LLC - Tập 39 - Trang 1-16 - 2017
Wenjing Yao1,2, Shengji Wang1, Boru Zhou1, Jianping Wang3, Tingbo Jiang1
1State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China
2Northeast Institute of Geography and Agroecology, Chinese Academy of Science, Harbin, China
3Department of agronomy, University of Florida, Gainesville, USA

Tóm tắt

The poplar transcription factor ERF76 gene is salt-inducible and plays an important role in regulating stress-related genes. To understand the regulatory mechanisms of ERF76 gene, the 1537-bp upstream DNA sequence of ERF76 gene was cloned from di-haploid Populus simonii × P. nigra. Sequence analysis indicated that the fragment contains a series of regulatory elements related to defense and stress responsiveness. The protein–DNA interaction assay indicated that the sequence interacted with proteins involved in oxidoreductase activity and plant defense and stress response. Biological evidence from promoter segmentation and deletion analysis demonstrated that the promoter segments in different length possess different transcriptional activity and respond to salt stress differentially in transgenic Arabidopsis. The characterization of ERF76 promoter provides mechanistic understanding of ERF76 gene in salt stress responses.

Tài liệu tham khảo

Aken OV, Zhang BT, Law S, Narsai R, Whelan J (2013) AtWRKY40 and AtWRKY63 modulate the expression of stress-responsive nuclear genes encoding mitochondrial and chloroplast proteins. Plant Physiol 162:254–271 Aoki H, Teramura H, Schepetilnikov M, Ryabova LA, Kusano H, Shimada H (2014) Enhanced translation of the downstream ORF attributed to a long 5′ untranslated region in the OsMac1 gene family members, OsMac2 and OsMac3. Plant Biotechnol 31:221–228 Bhullar S, Chakravarthy S, Advani S, Datta S, Pental D, Burma PK (2003) Strategies for development of functionally equivalent promoters with minimum sequence homology for transgene expression in plants: cis-elements in a novel DNA context versus domain swapping. Plant Physiol 132:988–998 Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254 Chakravarthy S, Tuori RP, D’Ascenzo MD, Fobert PR, Després C, Martin GB (2003) The tomato transcription factor Pti4 regulates defense-related gene expression via GCC box and non-GCC box cis elements. Plant Cell 15:3033–3050 Chaturvedi CP, Sawant SV, Kiran K, Mehrotra R, Lodhi N, Ansari SA, Tuli R (2006) Analysis of polarity in the expression from a multifactorial bidirectional promoter designed for high-level expression of transgenes in plants. J Biotechnol 123:1–12 Devi S, Chen X, Oliver DJ, Xiang C (2006) A novel high-throughput genetic screen for stress-responsive mutants of Arabidopsis thaliana reveals new loci involving stress responses. Plant J 47:652–663 Filichkin SA, Leonard JM, Alvaro M, Po-Pu L, Hiroyuki N (2004) A novel endo-β-mannanase gene in tomato leman5 is associated with anther and pollen development. Plant Physiol 134:1080–1087 Graham RT, Iain WM, John FG, Philip MG (2002) Arabidopsis thaliana GATA factors: organisation, expression and DNA-binding characteristics. Plant Mol Biol 50:43–57 He Y, Li W, Lv J, Jia YB, Wang MC, Xia GM (2011) Ectopic expression of a wheat MYB transcription factor gene, TaMYB73, improves salinity stress tolerance in Arabidopsis thaliana. J Exp Bot 63:1511–1522 Hehl R, Wingender E (2001) Database-assisted promoter analysis. Trends Plant Sci 6:251–255 Hernandez-Garcia CM, Bouchard RA, Rushton PJ et al (2010) High level transgenic expression of soybean (Glycine max) GmERF and Gmubi gene promoters isolated by a novel promoter analysis pipeline. BMC Plant Biol 10:1–16 Horstmann V, Huether CM, Jost W, Reski R, Decker EL (2004) Quantitative promoter analysis in Physcomitrella patens: a set of plant vectors activating gene expression within three orders of magnitude. BMC Biotechnol 4:1–13 Hou L, Chen LJ, Wang JY, Xu DF, Dai LX, Zhang H, Zhao YX (2012) Construction of stress responsive synthetic promoters and analysis of their activity in transgenic Arabidopsis thaliana. Plant Mol Biol Rep 30:1496–1506 Jeanette MQ, Paola B, Mats E, Sabeeha M (2000) Coordinate copper- and oxygen-responsive Cyc6 and Cpx1 expression in chlamydomonas is mediated by the same element. J Biol Chem 275:6080–6089 Jefferson RA (1987) Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol Biol Rep 5:387–405 Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: β-Glucuronidase as a sensitive and versatile gene marker in higher plants. EMBO J 6:3901–3907 Karthikeyan AS, Ballachanda DN, Raghothama KG (2009) Promoter deletion analysis elucidates the role of cis elements and 5′UTR intron in spatiotemporal regulation of AtPht1; 4 expression in Arabidopsis. Physiol Plant 136:10–18 Kim Y, Lee G, Jeon E et al (2014) The immediate upstream region of the 5′-UTR from the AUG start codon has a pronounced effect on the translational efficiency in Arabidopsis thaliana. Nucleic Acids Res 42:485–498 Lee SJ, Kang JY, Park HJ, Kim MD, Bae MS, Choi HI, Kim SY (2010) DREB2C interacts with ABF2, a bZIP protein regulating abscisic acid-responsive gene expression, and its overexpression affects abscisic acid sensitivity. Plant Physiol 153:716–727 Li ZM, Yang YJ, Yang F et al (2010) Cloning of LeGRP2 promoter from tomato that shows root-specific expression in Arabidopsis. Sci Agric Sin 43:1877–1882 Liu Y, Yin J, Xiao M et al (2013) Characterization of structure, divergence and regulation patterns of plant promoters. J Mol Biol Res 3:1885–1896 Park HC, Kim ML, Kang YH, Jeon JM, Yoo JH, Kim MC, Yoon HW (2004) Pathogen- and NaCl-induced expression of the SCaM-4 promoter is mediated in part by a GT-1 box that interacts with a GT-1-like transcription factor. Plant Physiol 135:2150–2161 Pesole G, Mignone F, Gissi C, Grillo G, Licciulli F, Liuni S (2001) Structural and functional features of eukaryotic mRNA untranslated regions. Gene 276:73–81 Plesch G, Ehrhardt T, Mueller-Roeber B (2001) Involvement of TAAAG elements suggests a role for Dof transcription factors in guard cell-specific gene expression. Plant J 28:455–464 Qin X, Zheng X, Shao C et al (2009) Stress-induced curcin-L promoter in leaves of Jatropha curcas L. and characterization in transgenic tobacco. Planta 230:387–395 Qiu P (2003) Recent advances in computational promoter analysis in understanding the transcriptional regulatory network. Biochem Biophys Res Commun 309:495–501 Sawant SV, Kiran K, Mehrotra R, Chaturvedi CP, Ansari SA, Singh P, Tuli R (2005) A variety of synergistic and antagonistic interactions mediated by cis-acting DNA motifs regulate gene expression in plant cells and modulate stability of the transcription complex formed on a basal promoter. J Exp Bot 56:2345–2353 Sharma N, Russell SD, Bhalla PL, Singh MB (2011) Putative cis-regulatory elements in genes highly expressed in rice sperm cells. BMC Res Notes 4:319 Shashikanth M, Krishna AR, Ramya G, Devi G, Ulaganathan K (2008) Genome-wide comparative analysis of Oryza sativa (japonica) and Arabidopsis thaliana 5'-UTR sequences for translational regulatory signals. Plant Biotechnol 25(6):553–563 Smieszek SP, Yang H, Paccanaro A et al (2014) Progressive promoter element combinations classify conserved orthogonal plant circadian gene expression modules. J R Soc Interface. doi:10.1098/rsif.2014.0535 Taha RS, Ismail I, Zainal Z, Akmar Abdullah SN (2012) The stearoyl-acyl-carrier-protein desaturase promoter (Des) from oil palm confers fruit-specific GUS expression in transgenic tomato. J Plant Physiol 169:1290–1300 Tatematsu K, Ward S, Leyser O, Kamiya Y, Nambara E (2005) Identification of cis-elements that regulate gene expression during initiation of axillary bud outgrowth in Arabidopsis. Plant Physiol 138:757–766 Tran LSP, Nakashima K, Sakuma Y, Simpson SD, Fujita Y, Maruyama K, Kazuo S, Yamaguchi-Shinozaki K (2004) Isolation and functional analysis of Arabidopsis stress-inducible NAC transcription factors that bind to a drought-responsive cis-element in the early responsive to dehydration stress 1 promoter. Plant Cell 16:2481–2498 Venter M, Botha FC (2004) Promoter analysis and transcription profiling: integration of genetic data enhances understanding of gene expression. Physiol Plant 120:74–83 Xu ZS, Ni ZY, Liu L et al (2008) Characterization of the TaAIDFa gene encoding a CRT/DRE-binding factor responsive to drought, high-salt, and cold stress in wheat. Mol Genet Genom 280:497–508 Yamaguchi-Shinozaki K, Shinozaki K (2005) Organization of cis-acting regulatory elements in osmotic-and cold-stress-responsive promoters. Trends Plant Sci 10:88–94 Yao WJ, Wang SJ, Zhou BR, Jiang TB (2016a) Transgenic poplar overexpressing endogenous transcription factor ERF76 gene improves salinity tolerance. Tree Physiol 36:896–908 Yao WJ, Wang L, Zhou BR, Wang SJ, Li RH, Jiang TB (2016b) Over-expression of poplar transcription factor gene ERF76 confers salt tolerance in transgenic tobacco. J Plant Physiol 198:23–31 Yao WJ, Zhang XM, Zhou BR, Zhao K, Li RH, Jiang TB (2017) Expression pattern of ERF gene family under multiple abiotic stresses in Populus simonii × P. nigra. Frontiers. Plant Sci 8:181. doi:10.3389/fpls.2017.00181 Zhang XR, Henriques R, Lin SS, Niu QW, Chua NH (2006) Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method. Nat Protoc 1:641–646 Zhang J, Zhang X, Wang Y, Hou H, Qian Y (2012) Characterization of sequence elements from malvastrum yellow vein betasatellite regulating promoter activity and DNA replication. Virol J 9:598–604