Characterization and light-induced expression of theS-adenosylmethionine decarboxylase gene fromIpomoea nil
Tóm tắt
We screened a genomic library of Japanese morning glory(Ipomoea nil) and isolated theMGSDCgi clone (GenBank Accession Number U64927). The genomic clone comprised four exons and three introns.MCSDCgi contained a 660-bp 5′-untranslated region (UTR), a 1089-bp coding region of 362 amino acids, and a 209-bp 3′-UTR. A TATA box sequence (TATATAA) was found at position -29. The transcript leader of thisSAMDC gene contained a short, upstream open reading frame (uORF) of 51 amino acids, which was interrupted with a 107-bp intron. Two other introns were positioned upstream of the uORF in the 5’-UTR. Six-day-old seedlings were grown under a 12-h light/dark cycle, then treated with white light The amount ofSAMDC transcript dramatically increased from the first hour after light exposure, then returned to a basal level.SAMDC mRNA was accumulated more under high rather than low irradiance. These results suggest a relationship between photo-response and the physiological role of polyamines (PAs). The level ofSAMDC mRNA was increased only by brassinosteroids, not by any other plant hormone such as kinetin, IAA, ABA, and GA. This hormonal response may have replaced the effect of light onSAMDC gene expression in the dark. PAs decreased theSAMDC mRNA level, which could then be restored by their inhibitors. This indicates thatSAMDC gene expression is regulated by its cellular contents.
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