Characterization and Mapping of a Shattering Mutant in Rice That Corresponds to a Block of Domestication Genes

Genetics - Tập 173 Số 2 - Trang 995-1005 - 2006
Hyeonso Ji1, Sang‐Ho Chu2, Wenzhu Jiang2, Young‐Il Cho2, Jang-Ho Hahn1, Moo-Young Eun1, Susan R. McCouch3, Hee‐Jong Koh2
1National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon 441-857, Korea
2Department of Plant Science, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Korea and
3Department of Plant Breeding, Cornell University, Ithaca, New York 14853-1901

Tóm tắt

Abstract Easy shattering reduces yield due to grain loss during harvest in cereals. Shattering is also a hindrance in breeding programs that use wild accessions because the shattering habit is often linked to desirable traits. We characterized a shattering mutant line of rice, Hsh, which was derived from a nonshattering japonica variety, Hwacheong, by N-methyl-N-nitrosourea (MNU) treatment. The breaking tensile strength (BTS) of the grain pedicel was measured using a digital force gauge to evaluate the degree of shattering of rice varieties at 5, 10, 15, 20, 25, 30, 35, and 40 days after heading (DAH). The BTS of Hwacheong did not decrease with increasing DAH, maintaining a level of 180–240 gf, while that of Hsh decreased greatly during 10–20 DAH and finally stabilized at 50 gf. Optical microscopy revealed that Hsh had a well-developed abscission layer similar to the wild rice Oryza nivara (accession IRGC105706), while Hwacheong did not produce an abscission layer, indicating that the shattering of Hsh was caused by differentiation of the abscission layer. On the basis of the BTS value and morphology of the abscission layer of F1 plants and segregation data in F2 populations, it was concluded that the easy shattering of Hsh was controlled by the single recessive gene sh-h. The gene sh-h was determined to be located on rice chromosome 7 by bulked segregant analysis. Using 14 SSR markers on rice chromosome 7, the gene sh-h was mapped between the flanking markers RM8262 and RM7161 at distances of 1.6 and 2.0 cM, respectively. An SSR marker Rc17 cosegregated with the gene sh-h. The locus sh-h for shattering was tightly linked to the Rc locus conferring red pericarp, as well as a QTL qSDs-7-1 for seed dormancy, implying that this region might represent a domestication block in the evolutionary pathway of rice.

Từ khóa


Tài liệu tham khảo

2001, Theor. Appl. Genet., 102, 118, 10.1007/s001220051626

2000, Theor. Appl. Genet., 100, 840, 10.1007/s001220051360

2002, Plant Cell Environ., 25, 479, 10.1046/j.1365-3040.2002.00839.x

2002, Crop Sci., 42, 2092, 10.2135/cropsci2002.2092

2005, Curr. Opin. Plant Biol., 8, 155, 10.1016/j.pbi.2005.01.005

1991, Genetics, 129, 285, 10.1093/genetics/129.1.285

1993, Genetics, 134, 559, 10.1093/genetics/134.2.559

1990, Rice Genet. Newsl., 7, 105

2002, J. Exp. Bot., 53, 2031, 10.1093/jxb/erf082

2000, Science, 289, 436, 10.1126/science.289.5478.436

1998, Breed. Sci., 48, 345

1994, Jpn. J. Breed., 44, 195, 10.1270/jsbbs1951.44.195

2004, Genetics, 166, 1503, 10.1534/genetics.166.3.1503

2005, Theor. Appl. Genet., 110, 1108, 10.1007/s00122-005-1939-2

2004, Theor. Appl. Genet., 108, 261, 10.1007/s00122-003-1432-8

1986, Jpn. J. Crop Sci., 55, 451, 10.1626/jcs.55.451

1982, Jpn. J. Crop Sci., 51, 271, 10.1626/jcs.51.271

1982, Jpn. J. Breed., 51, 43

1985, Jpn. J. Crop Sci., 54, 373, 10.1626/jcs.54.373

1982, Jpn. J. Crop Sci., 51, 542, 10.1626/jcs.51.542

1990, Jpn. J. Crop Sci., 59, 475, 10.1626/jcs.59.475

1995, Korean J. Crop Sci., 40, 103

2000, Genes Dev., 14, 108, 10.1101/gad.14.1.108

2002, Theor. Appl. Genet., 105, 1075, 10.1007/s00122-002-0988-z

1990, J. Agric. Sci. (Jpn.), 45, 186

2004, Theor. Appl. Genet., 109, 986, 10.1007/s00122-004-1710-0

1980, J. Korean Soc. Crop Sci., 25, 1

1987, Genomics, 1, 174, 10.1016/0888-7543(87)90010-3

2000, Nature, 404, 766, 10.1038/35008089

2000, Nature, 406, 910, 10.1038/35022611

2002, DNA Res., 9, 257, 10.1093/dnares/9.6.257

1991, Proc. Natl. Acad. Sci. USA, 88, 9828, 10.1073/pnas.88.21.9828

2002, Rice Genet. Newsl., 19, 74

1963, J. Fac. Agric. Hokkaido Univ., 53, 72

1970, Proc. Crop Sci. Soc. Jpn., 38, 425

1990, J. Breed., 40, 13, 10.1270/jsbbs1951.40.13

1995, Jpn. J. Crop Sci., 64, 607, 10.1626/jcs.64.607

1989, Crit. Rev. Plant Sci., 8, 103, 10.1080/07352688909382272

1995, Science, 269, 1714, 10.1126/science.269.5231.1714

1995, Proc. Natl. Acad. Sci. USA, 92, 6127, 10.1073/pnas.92.13.6127

2001, Plant Physiol., 126, 494, 10.1104/pp.126.2.494

2002, Theor. Appl. Genet., 104, 965, 10.1007/s00122-002-0889-1

2001, Curr. Biol., 11, 1914, 10.1016/S0960-9822(01)00593-0

2002, Annu. Rev. Plant Biol., 53, 131, 10.1146/annurev.arplant.53.092701.180236

1995

1984, J. Exp. Bot., 35, 1663

2006, Plant Cell, 18, 283, 10.1105/tpc.105.038430

2001, Genome Res., 11, 1441, 10.1101/gr.184001

2003, Theor. Appl. Genet., 107, 479, 10.1007/s00122-003-1270-8

2000, Euphytica, 115, 215, 10.1023/A:1004066416900

1999, Theor. Appl. Genet., 98, 243, 10.1007/s001220051064

Thông tin
Thông tin xuất bản

Genetics

Tập 173 Số 2

995-1005

Thông tin tác giả