Characteristics of gut microbiota and its response to a Chinese Herbal Formula in elder patients with metabolic syndrome

1. Eckel RH, Grundy SM, Zimmet PZ. The metabolic syndrome. Lancet. 2005; 365:1415-1428.

2. Gami AS, Witt BJ, Howard DE, Erwin PJ, Gami LA, Somers VK, Montori VM. Metabolic syndrome and risk of incident cardiovascular events and death: A systematic review and meta-analysis of longitudinal studies. J Am Coll Cardiol. 2007; 49:403-414.

3. Musso G, Gambino R, Cassader M. Interactions between gut microbiota and host metabolism predisposing to obesity and diabetes. Annu Rev Med. 2011; 62:361-380.

4. Ley RE, Turnbaugh PJ, Klein S, Gordon JI. Microbial ecology: Human gut microbes associated with obesity. Nature. 2006; 444:1022-1023.

5. Qin JJ, Li YR, Cai ZM, <i>et al</i>. A metagenome-wide association study of gut microbiota in type 2 diabetes. Nature. 2012; 490:55-60.

6. Xu J, Lian FM, Zhao LH, Zhao YF, Chen XY, Zhang X, Guo Y, Zhang C, Zhou Q, Xue Z, Pang X, Zhao L, Tong X. Structural modulation of gut microbiota during alleviation of type 2 diabetes with a Chinese herbal formula. ISME J. 2015; 9:552-562.

7. Chen KC, Lu R, Iqbal U, Hsu KC, Chen BL, Nguyen PA, Yang HC, Huang CW, Li YC, Jian WS, Tsai SH. Interactions between traditional Chinese medicine and western drugs in Taiwan: A population-based study. Comput Methods Programs Biomed. 2015; 122:462-470.

8. Zhang J. The clinical study of the Nourishing-Yin-and-Dredging-Collateral Formula&apos;s intervention in chronic renal damage towards elderly patients with matabolic syndrome. Nanjing: Nanjing University of Chinese Medicine; 2011. (in Chinese)

9. Zhu CL, Guo HM. Research on effect of Nourishing-Yin-and-Dredging-Collateral Formula on inflammatory mechanisms of human venous endothelial cells. Changchun Zhong Yi Yao Da Xue Xue Bao. 2014; 30:405-407. (in Chinese)

10. Expert Panel on Metabolic Syndrome of Chinese Diabetes Society. Recommendations on metabolic syndrome of Chinese diabetes society. Zhongguo Tang Niao Bing Za Zhi. 2004;12:156-161. (in Chinese)

11. Zoetendal EG, Akkermans ADL, De Vos WM. Temperature gradient gel electrophoresis analysis of 16S rRNA from human fecal samples reveals stable and host-specific communities of active bacteria. Appl Environ Microbial. 1998; 64:3854-3859.

12. Mu CL, Yang YX, Su Y, Zoetendal EG, Zhu WY. Differences in microbiota membership along the gastrointestinal tract of piglets and their differential alterations following an early-life antibiotic intervention. Front Microbiol. 2017; 8:797.

13. Caporaso JG, Kuczynski J, Stombaugh J, <i>et al</i>. QIIME allows analysis of high-throughput community sequencing data. Nat Methods. 2010; 7:335-336.

14. Cole JR, Wang Q, Cardenas E, Fish J, Chai B, Farris RJ, Kulam-Syed-Mohideen AS, McGarrell DM, Marsh T, Garrity GM, Tiedje JM. The Ribosomal Database Project: Improved alignments and new tools for rRNA analysis. Nucleic Acids Res. 2009; 37:D141-D145.

15. Lozupone C, Lladser ME, Knights D, Stombaugh J, Knight R. UniFrac: An effective distance metric for microbial community comparison. ISME J. 2011; 5:169-172.

16. Segata N, Izard J, Waldron L, Gevers D, Miropolsky L, Garrett WS, Huttenhower C. Metagenomic biomarker discovery and explanation. Genome Biol. 2011; 12:R60.

17. Xia J, Wishart DS. Using MetaboAnalyst 3.0 for comprehensive metabolomics data analysis. Curr Protoc Bioinformatics. 2016; 55:14.10.1-14.10.19.

18. Benjamini Y, Hochberg Y. Controlling the false discovery rate: A practical and powerful approach to multiple testing. J Roy Stat Soc B Met. 1995; 57:289-300.

19. Claesson MJ, Cusack S, O&apos;Sullivan O, <i>et al</i>. Composition, variability, and temporal stability of the intestinal microbiota of the elderly. Proc Natl Acad Sci USA. 2011; 108:4586-4591.

20. Milani C, Ticinesi A, Gerritsen J, <i>et al</i>. Gut microbiota composition and <i>Clostridium difficile</i> infection in hospitalized elderly individuals: A metagenomic study. Sci Rep. 2016; 6:25945

21. O&apos;Flaherty S, Saulnier DM, Pot B, Versalovic J. How can probiotics and prebiotics impact mucosal immunity? Gut Microbes. 2010; 1:293-300.

22. Le KA, Li Y, Xu XJ, Yang WT, Liu TT, Zhao X, Tang YG, Cai D, Go VL, Pandol S, Hui H. Alterations in fecal <i>Lactobacillus</i> and <i>Bifidobacterium</i> species in type 2 diabetic patients in Southern China population. Front Physiol. 2013; 3:496.

23. Karlsson FH, Tremaroli V, Nookaew I, Bergstrom G, Behre CJ, Fagerberg B, Nielsen J, Bäckhed F. Gut metagenome in European women with normal, impaired and diabetic glucose control. Nature. 2013; 498:99-103.

24. Larsen N, Vogensen FK, van den Berg FWJ, Nielsen DS, Andreasen AS, Pedersen BK, Al-Soud WA, Sørensen SJ, Hansen LH, Jakobsen M. Gut microbiota in human adults with type 2 diabetes differs from non-diabetic adults. Plos One. 2010; 5:e9085

25. Wang W, Chen LP, Zhou R, Wang XB, Song L, Huang S, Wang G, Xia B. Increased proportions of <i>Bifidobacterium</i> and the <i>Lactobacillus</i> group and loss of butyrateproducing bacteria in inflammatory bowel disease. J Clin Microbiol. 2014; 52:398-406.

26. Zeng H, Liu J, Jackson MI, Zhao FQ, Yan L, Combs GF Jr. Fatty liver accompanies an increase in <i>Lactobacillus</i> species in the hind gut of C57BL/6 mice fed a high-fat diet. J Nutr. 2013; 143:627-631.

27. Thevaranjan N, Puchta A, Schulz C, <i>et al</i>. Age-associated microbial dysbiosis promotes intestinal permeability, systemic inflammation, and macrophage dysfunction. Cell Host Microbe. 2017; 21:455-466.

28. Tsilingiri K, Barbosa T, Penna G, Caprioli F, Sonzogni A, Viale G, Rescigno M. Probiotic and postbiotic activity in health and disease: Comparison on a novel polarised ex-vivo organ culture model. Gut. 2012; 61:1007-1015.

29. Du XN, Xu HJ, Huang PP, Dai JX. Diversity and antimicrobial activity of endophytic bacteria isolated from <i>Lycium barbarum</i> of Ningxia. Wei Sheng Wu Xue Tong Bao. 2015; 42:1779-1787. (in Chinese)

30. Wang YF. Effects and mechanisms of polysaccharide from <i>Polygonatum sibiricum</i> on lipid metabolism disorder in rats. Yunnan Traditional Chinese Medicine College, 2017. Thesis. (in Chinese)

31. Wang L. Effects of stachyose in rehmannia on intestinal microflora in diabetic mice. Peking Union Medical College, 2013. Thesis. (in Chinese)

32. Lv BY, Fan RX.The composition and function of <i>Lycium barbarum</i> L. Hebei Zhong Yi Yao Xue Bao. 1998; 13:29-30.(in Chinese)

33. Liu YT, Xu W, Xin Y, Li XL. Regulating effects of Fructus Lycii extracts on dysbiosis of intestinal flora of mice. Tian Ran Chan Wu Yan Jiu Yu Kai Fa. 2015; 27:1270-1272,1295. (in Chinese)

34. Xu J, Lian F, Zhao L, Zhao Y, Chen X, Zhang X, Guo Y, Zhang C, Zhou Q, Xue Z, Pang X, Zhao L, Tong X. Structural modulation of gut microbiota during alleviation of type 2 diabetes with a Chinese herbal formula. ISME J. 2015; 9:552-562.