Changes with time in the distribution of virus and PR protein around single local lesions of TMV infected tobacco
Tóm tắt
ELISA was used to determine PR la protein and TMV accumulation in local necrotic lesions produced on salicylic acid and water sprayed Nicotiana tabacum cv Xanthi-nc leaves. The amount of PR la protein produced is the result of an interaction between the salicylic acid treatment and lesion growth. The implication of these observations for experiments investigating the relationship between PR proteins and resistance are discussed. The distribution of TMV and PR la protein in and around single local necrotic lesions up to 14 days after inoculation was measured by ELISA. The highest concentration of TMV was in the centre of the lesion and decreased rapidly with distance from the centre. In contrast there was very little PR la protein in the centre of the lesion, the largest amounts were just outside the centre, and the concentration then decreased with distance from the centre. This is the distribution that might be expected for a substance closely associated with the restriction of virus spread.
Tài liệu tham khảo
Antoniw JF, Pierpoint WS: The purification and properties of one of the ‘b’ proteins from virus-infected tobacco plants. Journal of General Virology 39:343–350, 1978.
Antoniw JF, Ritter CE, Pierpoint WS, Van Loon LC: Comparison of three pathogenesis-related proteins from plants of two cultivars of tobacco infected with TMV. Journal of General Virology 47:79–87, 1980.
Antoniw JF, White RF, Barbara DJ, Jones P, Longley A: The detection of PR (b) protein and TMV by ELISA in systemic and localised virus infections of tobacco. Plant Molecular Biology 4:55–60, 1985.
Barbara DJ, Clark MF: A simple indirect ELISA using F(ab')2 fragments of immunoglobulin. Journal of General Virology 58:315–322, 1982.
Gianinazzi S, Martin C, Vallee JC: Hypersensibilité aux virus, température et protéines solubles chez le Nicotiana Xanthi n.c. Apparition de nouvelles macromolécules lors de la repression de la synthèse virale. Cr Acad Sci Paris D 270:2382–2386, 1970.
Rohloff H, Lerch B: Soluble leaf proteins in virus infected plants and acquired resistance. Phytopathologische Zeitschrift 89:306–316, 1977.
Van Loon LC: Regulation of changes in proteins and enzymes associated with active defence against virus infection. In RKS Wood (Editor) Active defence mechanisms in plants 247–273. Plenum Press, New York 1982.
Van Loon LC, Antoniw JF: Comparison of the effects of salicylic acid and ethephon with virus-induced hypersensitivity and acquired resistance in tobacco. Netherland Journal of Plant Pathology 88:237–256, 1982.
White RF: Acetylsalicylic acid (aspirin) induces resistance to tobacco mosaic virus in tobacco. Virology 99:410–412, 1979.