Changes in glycoconjugates revealed by lectin staining and stage-specific embryonic antigen-1 immunostaining in hamster submandibular glands during the postnatal period
Tóm tắt
Lectin binding and stage-specific embryonic antigen-1 (SSEA-1) immunoreactivity were studied in the developing submandibular glands of young Syrian golden hamsters (Mesocricetus auratus) from postnatal day 1 (the day of birth) to day 28. The submandibular glands were fixed in a solution containing 6% mercuric chloride, 1% sodium acetate, and 0.1% glutaraldehyde (HgCl2-G) or 4% paraformaldehyde (4P), and embedded in paraffin. Sections from HgCl2-G fixation were stained with three lectin-peroxidase conjugates: peanut agglutinin (PNA), Ulex europeus I agglutinin (UEA I), and wheat germ agglutinin (WGA). Sections from the 4P-fixed tissues were immunostained with monoclonal antibodies against SSEA-1, sialyl SSEA-1 and fucosyl SSEA-1. On the day of birth, the terminal unit of the submandibular gland was composed of fetal type secretory cells and proacinar cells. The secretory cells were PNA, UEA I, and WGA positive. The number of secretory terminal tubule cells decreased rapidly, and lectin-positive secretory cells were replaced by adult secretory cells that did not show PNA or UEA I stainings but were weakly positive for WGA. Fetal secretory cells were positively immunostained for SSEA-1 and sialyl SSEA-1, and immature ductal cells were stained for fucosyl SSEA-1. The positive stainings disappeared with regression of the fetal epithelial cells. Hence, modulation of glycoconjugate expression in the submandibular glands, which reflects changes in secretory cells from the fetal type to adult type during postnatal development, is revealed by lectin staining and immunostaining for SSEA-1 and related antigens.
Tài liệu tham khảo
Abe K, McKibbin JM, Hakomori S (1983) The monoclonal antibody directed to difucosylated type 2 chain (Fucα1→ 2Galβ1→4[Fucα1→3]GlcNAc; Y determinant). J Biol Chem 258:11793–11797
Calderó J, Campo E, Calomarde X, Torra M (1988) Distribution and changes of glycoconjugates in rat colonic mucosa during development. A histochemical study using lectins. Histochemistry 90:261–270
Colony PC, Steely J (1987) Lectin binding patterns in developing rat colon. Gastroenterology 92:1116–1126
Cutler LS, Chaudhry AP (1974) Cytodifferentiation of the acinar cells of the rat submandibular gland. Dev Biol 41:31–41
Damjanov I (1987) Lectin cytochemistry and histochemistry. Lab Invest 57:5–20
Denny PA, Pimprapaiporn W, Kim MS, Denny PC (1988) Quantitation and localization of acinar cell-specific mucin in submandibular glands of mice during postnatal development. Cell Tissue Res 251:381–386
Devi NS, Jacoby F (1966) The submandibular gland of the golden hamster and its post-natal development. J Anat 100:269–285
Dvorák M (1969) The secretory cells of the submaxillary gland in the perinatal period of development in the rat. Z Zellforsch 99:346–356
Feizi T (1985) Demonstration by monoclonal antibodies that carbohydrate structures of glycoproteins and glycolipids are onco-developmental antigens. Nature 314:53–57
Fox N, Damjanov I, Martinez-Hernandez A, Knowles BB, Solter D (1981) Immunohistochemical localization of the early embryonic antigen (SSEA-1) in postimplantation mouse embryos and fetal and adult tissues. Dev Biol 83:391–398
Fox N, Damjanov I, Knowles BB, Solter D (1983) Immunohisto-chemical localization of the mouse stage-specific embryonic antigen 1 in human tissues and tumors. Cancer Res 43:669–678
Fukushi Y, Nudelman E, Levery SB, Hakomori S (1984) Novel fucolipids accumulating in human adenocarcinoma. III. a hybridoma antibody (FH6) defining a human cancer-associated difucoganglioside (VI3NeuAcV3III3Fuc2nLc6). J Biol Chem 259:10511–10517
Fukushi Y, Kannagi R, Hakomori S, Shepard T, Kulander BG, Singer JW (1985) Location and distribution of difucoganglioside (VI3NeuAcV3III3Fuc2nLc6) in normal and tumor tissues defined by its monoclonal antibody FH6. Cancer Res 45:3711–3717
Gallagher JT, Corfield AP (1978). Mucin-type glycoproteins-new perspectives on their structure and synthesis. Trends Biochem Sci 3:38–41
Goldstein IJ, Poretz RD (1986) Isolation, physicochemical characterization, and carbohydrate-binding specificity of lectins. In: Liener IE, Sharon N. Goldstein U (eds) The lectins. Properties, functions, and applications in biology and medicine. Academic Press, Orlando, pp 33–243
Gooi HC, Feizi T, Kapadia A, Knowles BB, Solter D, Evans MJ (1981) Stage-specific embryonic antigen involves α1→2 fucosylated type 2 blood group chains. Nature 292:156–158
Gresik EW, MacRae EK (1975) The postnatal development of the sexually dimorphic duct system and of amylase activity in the submandibular glands of mouse. Cell Tissue Res 157:411–422
Hakomori S, Nudelman E, Levery S, Solter D, Knowles BB (1981) The hapten structure of a developmentally regulated glycolipid antigen (SSEA-1) isolated from human erythrocytes and adenocarcinoma: a preliminary note. Biochem Biophys Res Commun 100:1578–1586
Hauke Ch, Horn R, Breuer W, Sinowatz F (1989) Postnatal development of lectin binding sites in the rat ventral prostate. Histochem 121:651–658
Ito K, Ito T, Strum JM, McDowell EM (1991) Lectin histochemistry of developing submandibular glands of fetal Syrian golden hamsters. Anat Embryol 183:135–141
Ivatt RJ (1984) Role of glycoproteins during early mammalian embryogenesis. In: Ivatt RJ (ed) The biology of glycoprotein. Plenum Press, New York, pp 95–181
Jacoby F, Leeson CR (1959) The postnatal development of the rat submaxillary gland. J Anat 93:201–216
Kannagi R, Nudelman E, Levery SB, Hakaomori S (1982) A series of human erythrocyte glycosphingolipids reacting to the monoclonal antibody directed to a developmentally regulated antigen, SSEA-1. J Biol Chem 257:14865–14874
Kannagi R, Fukushi Y, Tachikawa T, Noda A, Shin S, Shigeta K, Hiraiwa N, Fukuda Y, Inamoto T, Hakomori S, Imura H (1986) Quantitative and qualitative characterization of cancerassociated serum glycoprotein antigens expressing fucosyl or syalosyl-fucosyl type 2 chain polylactosamine. Cancer Res 46:2619–2626
Lowe JB, Stoolman LM, Nair RP, Larsen RD, Berhend TL, Marks RM (1990) ELAM-1-dependent cell adhesion to vascular endothelium determined by a transfected human fucosyltransferase cDNA. Cell 63:475–484
Malmi R, Fröjdman K, Söderström K-O (1990) Differentiation-related changes in the distribution of glycoconjugates in rat testis. Histochemistry 94:387–395
Miyake M, Zenita K, Tanaka O, Okada Y, Kannagi R (1988) Stage-specific expression of SSEA-1-related antigens in the developing lung of human embryos and its relation to the distribution of these antigens in lung cancers. Cancer Res 48:7150–7158
Moreira JE, Ball WD, Mirels L, Hand AR (1991) Accumulation and localization of two adult acinar cell secretory protein during development of the rat submandibular gland. Am J Anat 191:167–184
Muramatsu T (1988) Developmentally regulated expression of cell surface carbohydrates during mouse embryogenesis. J Cell Biochem 36:1–14
Pereira MEA, Kisailus EC, Gruezo F, Kabat EA (1978) Immunochemical studies on the combinding site of the blood group H-specific lectin 1 from Ulex europeus seeds. Arch Biochem Biophys 185:108–115
Phillips ML, Nudelman E, Gaeta FCA, Perez M, Singhal AK, Hakomori S, Paulson JC (1990) ELAM-1 mediates cell adhesion by recognition of a carbohydrate ligand, sialyl-Lex. Science 250:1130–1132
Schulte BA, Spicer SS (1983) Light microscopic detection of sugar residues in glycoconjugates of salivary glands and the pancreas with lectin-horseradish peroxidase conjugates. I. Mouse. Histochem J 15:1217–1238
Solter D, Knowles BB (1978) Monoclonal antibody defining a stage-specific mouse embryonic antigen (SSEA-1). Proc Natl Acad Sci USA 75:5565–5569
Taatjes DJ, Roth J (1990) Selective loss of sialic acid from rat small intestinal epithelial cells during postnatal development: demonstration with lectin-gold techniques. Eur J Cell Biol 53:255–266
Waltz G, Aruffo A, Kolanus W, Bevilacqua M, Seed B (1990) Recognition by ELAM-1 of the sialyl-Lex determinant on myeloid and tumor cells. Science 250:1132–1135
Yamashina S, Barka T (1973) Development of endogenous peroxidase in fetal rat submandibular gland. J Histochem Cytochem 21:42–50
Yohro T (1970) Development of secretory units of mouse submandibular gland. Z Zellforsch 110:173–184
Zenita K, Kirihata Y, Kitahara A, Shigeta K, Higuchi K, Hirashima K, Murachi T, Miyake M, Takeda T, Kannagi R (1988) Fucosylated type 2 chain polylactosamine antigens in human lung cancer. Int J Cancer 41:344–349
Zhou XH, Kawakami H, Hirano H (1992) Changes in lectin binding patterns of Leydig cells during fetal and postnatal development in mice. Histochem J 24:354–360