Changes in free polyamine concentration induced by salt stress in seedlings of different species

Plant Growth Regulation - Tập 56 - Trang 167-177 - 2008
Pedro Javier Zapata1, Maria Serrano2, Maria Teresa Pretel2, Maria Angeles Botella2
1Departamento Tecnología Agroalimentaria, Escuela Politécnica Superior de Orihuela, Universidad Miguel Hernández, Orihuela, Spain
2División Fisiología Vegetal, Departamento Biología Aplicada, Escuela Politécnica Superior de Orihuela, Universidad Miguel Hernández, Orihuela, Spain

Tóm tắt

Growth rate, mineral composition and changes in polyamine concentration induced in response to salinity were studied in six crop species: spinach, lettuce, bean, pepper, beetroot and tomato. Salinity decreased growth rate, but sensitivity differed amongst the species: pepper being the most sensitive, followed by bean, tomato, lettuce and spinach, with beetroot being the most tolerant. The increase of Na+ and total cation with salinity in shoots was the highest in spinach and beetroot, the most tolerant species, while in pepper it was the lowest. Changes in putrescine (Put) concentration in shoots were related to salinity tolerance (increased in the most sensitive), while changes in spermidine (Spd; decreases) and spermine (Spm; increases) were similar with most species, except for pepper in which salinity strongly increased Put, Spd and Spm. Therefore, total polyamine concentration increased in pepper shoot, while it decreased in the other species. Thus, results show that Put accumulation was a consequence of salt stress in the most sensitive species, while salt tolerant species (beetroot) showed little change in polyamine concentration, and higher concentration in both Na+ and total cations. The role of polyamines or cation increased concentration after saline treatment in species with different salt tolerance is discussed.

Tài liệu tham khảo

Ashraf M, Harris PJC (2004) Potential biochemical indicators of salinity tolerance in plants. Plant Sci 166:3–16. doi:10.1016/j.plantsci.2003.10.024 Aziz A, Martin-Tanguy J, Larher F (1999) Salt stress-induced proline accumulation and changes in tyramine and polyamine levels are linked to ionic adjustment in tomato leaf discs. Plant Sci 145:83–91. doi:10.1016/S0168-9452(99)00071-0 Basu R, Maitra N, Ghosh B (1988) Salinity result in polyamine accumulation in early rice (Oryza sativa L.) seedlings. Aust J Plant Physiol 15:777–786 Blom-Zandstra M, Vogelzang SA, Veen BW (1998) Sodium fluyes in sweet pepper exposed to varying sodium concentrations. J Exp Bot 49:1863–1868. doi:10.1093/jexbot/49.328.1863 Botella MA, del Amor F, Amorós A, Serrano M, Martínez V, Cerdá A (2000) Polyamine, ethylene and other physico-chemical parameters in tomato (Lycopersicum esculentum) fruits as affected by salinity. Physiol Plant 109:428–434. doi:10.1034/j.1399-3054.2000.100409.x Bouchereau A, Aziz A, Larher F, Martin-Tanguy J (1999) Polyamines and environmental challenges: recent development. Plant Sci 140:103–125. doi:10.1016/S0168-9452(98)00218-0 Chartzoulakis K, Klapaki G (2000) Response of two greenhouse pepper hybrids to NaCl salinity during different growth stages. Sci Hortic (Amsterdam) 86:247–260. doi:10.1016/S0304-4238(00)00151-5 Cornillon P, Palloix A (1997) Influence of sodium chloride on the growth and mineral nutrition of pepper cultivars. J Plant Nutr 20:1085–1094 Coughlan SJ, Wyn Jones RG (1980) Some responses of Spinacea oleracea to salt stress. J Exp Bot 31:883–893. doi:10.1093/jxb/31.4.883-a Cramer GR, Alberico GJ, Schmidt C (1994) Leaf expression limits dry matter accumulation of salt-stressed maize. Aust J Plant Physiol 21:663–674 Das S, Bose A, Ghosh B (1995) Effect of salt stress on polyamine metabolism in Brassica campestris. Phytochemistry 39:283–285. doi:10.1016/0031-9422(94)00920-O Flores HE (1991) Changes in polyamine metabolism in response to abiotic stress. In: Slocum RM, Flores HE (eds) Biochemistry and physiology of polyamines plants. CRC Press, Boca Raton, pp 213–228 Flowers TJ, Hajibagheril MA (2001) Salinity tolerance in Hordeum vulgare: ion concentrations in root cells of cultivars differing in salt tolerance. Plant Soil 231:1–9. doi:10.1023/A:1010372213938 Francois LE, Maas EV, Donovan TJ, Youngs VL (1986) Effects of salinity on grain yield and quality, vegetative growth, and germination of semi-dwarf and durum wheat. Agron J 78:1053–1058 Ghoulam C, Foursy A, Fares K (2002) Effects of salt stress on growth, inorganic ions and proline accumulation in relation to osmotic adjustment in five sugar beet cultivars. Environ Exp Bot 47:39–50. doi:10.1016/S0098-8472(01)00109-5 Gonzalez LM, Ramírez R (1999) Respiration, water relations and pigment concentrations in rice seedlings grown under saline conditions. Cultivos Tropicales 20:35–37 Gzik A (1996) Accumulation of proline and pattern of alpha-amino acids in sugar beet plants in response to osmotic, water and salt stress. Environ Exp Bot 36:29–38. doi:10.1016/0098-8472(95)00046-1 Heuer B, Plaut Z (1989) Photosynthesis and osmotic adjustment of two sugarbeet cultivars grown under saline conditions. J Exp Bot 40:437–440. doi:10.1093/jxb/40.4.437 Iqbal M, Ashraf M (2005) Changes in growth, photosynthetic capacity and ionic relations in spring wheat (Triticum aestivum L.) due to pre-sowing seed treatment with polyamines. Plant Growth Regul 46:19–30. doi:10.1007/s10725-005-5901-8 Kakkar RR, Rai VR (1997) Polyamines under salt stress. In: Jaiwl PK, Singh RP, Gulati A (eds) Strategies for improving salt tolerance in higher plants. Science Publ Enfield, USA, pp 191–203 Kakkar RK, Bhaduri S, Rai VK, Kumar S (2000) Amelioration of NaCl stress by arginine in rice seedlings: changes in endogenous polyamines. Biol Plant 43:419–422. doi:10.1023/A:1026715032115 Katiyar S, Dubey RS (1990a) Changes in polyamine titer in rice seedlings following NaCl salinity stress. J Agron Crop Sci 165:19–27. doi:10.1111/j.1439-037X.1990.tb00830.x Katiyar S, Dubey RS (1990b) Salinity-induced accumulation of polyamines in germinating rice seeds differing in salt tolerance. Crop Sci 30:229–240 Kirsch M, Zhigang A, Viereck R, Low R, Rausch T (1996) Salt stress induces an increased expression of V-type H+-ATPase in mature sugar beet leaves. Plant Mol Biol 32:543–547. doi:10.1007/BF00019107 Krishnamurthy R, Bhagwat KA (1989) Polyamines as modulators of salt tolerance in rice cultivars. Plant Physiol 91:500–504 Lauchli A (1990) Calcium, salinity and the plasma membrane. In: Leonard RT, Hepler PK (eds) Calcium in plant growth. The American Society of Plant Physiologists, Rockville, pp 26–35 Lin CC, Kao CH (1995) Levels of endogenous polyamines and NaCl inhibited growth of rice seedlings. Plant Growth Regul 17:15–20 Ltifi A, Van der Beek JG (1992) Salt tolerance in pepper (Capsicum annum L.): K+/Na+ ratio as a discrimination character for tolerance. Capsicum Newsletter special issue. Rome, Italy, pp 51–56 Maas EV, Hoffman GF (1977) Crop salt tolerance-current-assessment. J Irr Drain Div-ASCE 103:115–134 Mansour MMF (1997) Cell permeability under salt stress. In: Jaiwl PK, Singh RP, Gulati A (eds) Strategies for improving salt tolerance in higher plants. Science Publ, Enfield, pp 87–110 Mansour MMF (2000) Nitrogen containing compounds and adaptation of plants to salinity stress. Biol Plant 43:491–500. doi:10.1023/A:1002873531707 Mansour MMF, Al-Mutawa MM (1999) Stabilization of plasma membrane by polyamines against salt stress. Cytobios 100:7–17 Mansour MMF, Salama KHA (2004) Cellular basis of salinity tolerance in plants. Environ Exp Bot 52:113–122. doi:10.1016/j.envexpbot.2004.01.009 Mansour MMF, Salama KHA, Ali FZM, Abou Hadid AF (2005) Cell and plant responses to NaCl in Zea mays L. cultivars differing in salt tolerance. Genet App Plant Physiol 31:29–41 Mennen H, Jacoby B, Marschner H (1990) Is sodium/proton antiport ubiquitous in plant cells? J Plant Physiol 137:180–183 Munns R (2002) Comparative physiology of salt and water stress. Plant Cell Environ 25:239–250. doi:10.1046/j.0016-8025.2001.00808.x Munns R, Schachtman DP, Condon AG (1995) The significance of a two-phase growth response to salinity in wheat and barley. Aust J Plant Physiol 22:561–569 Navarro JM, Garrido C, Martínez V, Carvajal M (2003) Water relations and xylem transport of nutrients in pepper plants grown under two different salt stress regimes. Plant Growth Regul 41:237–245. doi:10.1023/B:GROW.0000007515.72795.c5 Ndayiragije A, Lutts S (2007) Long term exogenous putrescine application improves grain yield of a salt-sensitive rice cultivar exposed to NaCl. Plant Soil 291:225–238. doi:10.1007/s11104-006-9188-y Pitman MG (1975) Whole plants. In: Baker DA, Hal JL (eds) Ion transport in plant cells and tissues. North-Holland Publishing Company, Amsterdam, pp 267–308 Pitman MG, Lauchli A (2002) Global impact of salinity and agricultural ecosystems. In: Lauchli A, Luttge V (eds) Salinity: environment-plants molecules. Kluwer, Netherlands, pp 3–20 Reggiani R, Bozo S, Bertani A (1994) Changes in polyamine metabolism in seedlings of three wheat (Triticum aestivum L.) cultivars differing in salt sensitivity. Plant Sci 102:121–126. doi:10.1016/0168-9452(94)90028-0 Tester M, Davenport R (2003) Na+ tolerance and Na+ transport in higher plants. Ann Bot (Lond) 91:503–527. doi:10.1093/aob/mcg058 Yeo A (1998) Molecular biology of salt tolerance in the context of whole-plant physiology. J Exp Bot 49:915–929. doi:10.1093/jexbot/49.323.915 Zacchini M, Marotta A, de Agazio M (1997) Tolerance to salt stress in maize callus lines with different polyamine content. Plant Cell Rep 17:119–122. doi:10.1007/s002990050363 Zapata PJ, Serrano M, Pretel MT, Amoros A, Botella MA (2003) Changes in ethylene evolution and polyamine profiles of seedlings of nine cultivars of Lactuca sativa L. in response to salt stress during germination. Plant Sci 164:557–563. doi:10.1016/S0168-9452(03)00005-0 Zapata PJ, Serrano M, Pretel MT, Amoros A, Botella MA (2004) Polyamines and ethylene changes during germination of different plant species under salinity. Plant Sci 167:781–788. doi:10.1016/j.plantsci.2004.05.014 Zhao FG, Qin P (2004) Protective effect of exogenous polyamines on root tonoplast function against salt stress in barley seedlings. Plant Growth Regul 42:97–103. doi:10.1023/B:GROW.0000017478.40445.bc