Cerium-based ultracytochemical localization of aspartate transcarbamylase activity in the cell membrane complex of Saccharomyces cerevisiae

Micron - Tập 28 - Trang 221-230 - 1997
Josef Voříšek1, Nicole Gas2, Michele Denis-Duphil3
1Institute of Microbiology, Academy of Sciences of Czech Republic, Vídeňská 1083, CZ 142 20, Praha 4, Czech Republic
2Laboratoire de Biologie Moléculaire des Eucaryotes, Institut de Biologie Céllulaire et de Génétique, UPR 9006-CNRS, Complexe scientifique de Rangueil, F 31077, Toulouse, Cedex 4, France
3Centre de Bioingenierie Gilbert Durand, UMR-CNRS 5504, Complexe scientifique de Rangueil, F 31077, Toulouse, Cedex 4, France

Tài liệu tham khảo

Aibangbee, 1992, Location of pyrimidine pathway enzymes in higher plant cells, 65 Angelmüller, 1995, Localization of dihydroorotate oxidase in myocardium and kidney cortex of the rat. An electron microscopic study using the cerium technique, Histochemistry, 103, 287, 10.1007/BF01457413 Araki, 1993, The cerium based method is advantageous in combination of phosphatase enzyme cytochemistry with postembedding immunochemistry, Acta Histochem. Cytochem., 26, 569, 10.1267/ahc.26.569 Bauer, 1975, The lipid globules of Saccharomyces cerevisae: a combined chemical and ultrastructural study using ultrathin frozen sections, J. Ultrastruct. Res., 51, 32, 10.1016/S0022-5320(75)80005-0 Bernhardt, 1972, Carbamoyl phosphate compartmentation in Neurospora: histochemical localization of aspartate and ornithine transcarbamoylases, 69, 1868 Bottomley, 1967, Subcellular distribution of rat ATCase, Biochimica Biophysica Acta, 148, 588, 10.1016/0304-4165(67)90166-3 Bradford, 1976, A rapid and sensitive method for the quantification of microgram quantities utilizing the principle of protein dye binding, Anal. Biochem., 72, 248, 10.1016/0003-2697(76)90527-3 Chaparian, 1988, Intracellular location of the multidomain protein CAD in mammalian cells, The FASEB J. Res. Commun., 2, 2982, 10.1096/fasebj.2.14.2903106 Costerton, 1981, The bacterial glycocalyx in nature and disease, Annual Review of Microbiology, 35, 299, 10.1146/annurev.mi.35.100181.001503 Davidson, 1990, Organization and nucleotide sequence of the 3′ end of the human CAD gene, DNA Cell Biol., 9, 667, 10.1089/dna.1990.9.667 Denis-Duphil, 1989, Pyrimidine biosynthesis in Saccharomyces cerevisiae: the URA2 cluster gene, its multifunctional enzyme product and other structural or regulatory genes involved in de novo UMP synthesis, Biochem. Cell. Biol., 67, 612, 10.1139/o89-094 Denis-Duphil, 1990, Yeast carbamoyl-phosphate-synthase-aspartate-transcaramylase multidomain protein is phosphorylated in vitro by cAMP-dependent protein kinase, European Journal of Biochemistry, 193, 581, 10.1111/j.1432-1033.1990.tb19376.x Van Duijn, 1973, Fundamental aspects in enzyme cytochemistry, 3 Evans, 1986, CAD, a chimeric protein that initiates de novo pyrimidine synthesis in higher eukaryotes, 283 Faure, 1989, Molecular characterization of Dictyostelium discoideum gene encoding multifunctional enzyme of the pyrimidine pathway, European Journal of Biochemistry, 179, 345, 10.1111/j.1432-1033.1989.tb14560.x Hugon, 1973, Lead salt methods, 3 Hulstaert, 1992, Electron microscopical demonstration of alkaline phosphatase activity with the cerium based method in citrate-containing medium at pH 9.3 and the influence of glutaraldehyde fixation, Cell. Mol. Biol., 38, 545 Jarry, 1978, Purification of aspartate transcarbamylase from Drosophila melanogaster, European Journal of Biochemistry, 87, 533, 10.1111/j.1432-1033.1978.tb12404.x Krungkrai, 1991, Purification and characterization of dihydroorotate dehydrogenase from the rodent malaria parasite Plasmoidum berghei, Biochemistry, 30, 1934, 10.1021/bi00221a029 Kulaev, 1975, Biochemistry of inorganic polyphosphates, Rev. Physiol. Biochem. Pharmacol., 73, 131, 10.1007/BFb0034661 Lacroute, 1968, Regulation of pyrimidine biosynthesis in Saccharomyces cerevisiae, Journal of Bacteriology, 95, 824, 10.1128/JB.95.3.824-832.1968 Linnemans, 1977, Localization of acid phosphatase in Saccharomyces cerevisiae: a clue to cell wall formation, Journal of Bacteriology, 131, 638, 10.1128/JB.131.2.638-644.1977 Nagy, 1982, Nuclear localization of aspartate transcarbamylase in Saccharomyces cerevisiae, Journal of Cellular Biology, 92, 790, 10.1083/jcb.92.3.790 Nagy, 1989, The primary structure of the aspartate transcarbamylase region of the URA2 gene product in Saccharomyces cerevisiae. Features involved in activity and in nuclear localization, Journal of Biological Chemistry, 264, 8366, 10.1016/S0021-9258(18)83191-X Nagy, 1992, Divergent evolution of pyrimidine biosynthesis between anaerobic and aerobic, 89, 8966 Van Noorden, 1993, Cerium methods for light and electron microscopical histochemistry, Journal of Microscopy (Oxford), 171, 3, 10.1111/j.1365-2818.1993.tb03354.x Orlovich, 1993, Polyphosphate granules are an artefact of specimen preparation in the ectomycorrhizal fungus Psilithus tinctorium, Protoplasma, 173, 91, 10.1007/BF01378998 Penverne, 1983, In situ behavior or the pyrimidine pathway enzymes in Saccharomyces cerevisiae, Arch. Biochem. Biophys., 225, 562, 10.1016/0003-9861(83)90068-1 Poon, 1994, Thymidilate synthase is localized to the nuclear periphery in the yeast Saccharomyces cerevisiae, Journal of Biological Chemistry, 269, 8341, 10.1016/S0021-9258(17)37199-5 Robinson, 1985, Improved localization of intracellular sites of phosphatases using cerium and cell permeabilization, Journal of Histochemistry and Cytochemistry, 33, 749, 10.1177/33.8.2991362 Ryan, 1974, Subcellular localization of isoleucine-valine biosynthetic enzymes in yeast, Journal of Bacteriology, 120, 631, 10.1128/JB.120.2.631-637.1974 Shibata, 1986, Localization of carbamoyl-phosphate synthetase and aspartate carbamoylase in chloroplasts, Plant Physiology, 80, 126, 10.1104/pp.80.1.126 Strouboulis, 1996, Functional compartmentation of the nucleus, Journal of Cell Science, 109, 1991, 10.1242/jcs.109.8.1991 Voříšek, 1977, Electron-cytochemical demonstration of acid phosphatase in saprophytic Claviceps purpurea producing clavine alkaloids, Arch. Microbiol., 111, 189, 10.1007/BF00549368 Voříšek, 1986, Ultracytochemical localization of X-prolyl-dipeptidyl (amino)peptidase in microglobules and endoplasmic membranes accumulated in pep4-3 mutant of Saccharomyces cerevisiae, Histochemistry, 84, 87, 10.1007/BF00493426 Voříšek, 1989, Ultracytochemical localization of the vacuolar marker enzymes alcaline phosphatase, adenosine triphosphatase, carboxypeptidase Y and aminopeptidase reveal new concept of vacuole biogenesis in Saccharomyces cerevisiae, Histochemistry, 92, 421, 10.1007/BF00492500 Voříšek, 1994, Activity of kex2 dibasic endoprotease is localized throughout the secretory pathway in Saccharomyces cerevisiae. An ultracytochemical study, European Journal of Cell Biology, 63, 130 Voříšek, 1984, Gomori staining of cellular phosphates in yeast-interference of polyphosphatases, Histochemistry Journal, 16, 338, 10.1007/BF01002843 Voříšek, 1982, Electron-cytochemical study of lipid deposit condensation and degradation in ascomycetes—self assembly of unit membranes, Cellular and Molecular Biology, 28, 221 Voříšek, 1982, Fine cytochemical localization of polyphosphates in the yeast Saccharomyces cerevisiae, Zbl. Mikrobiol., 137, 421 Voříšek, 1993, Ultracytochemical localization of dihydroorotate dehydrogenase in mitochondria and vacuoles of Saccharomyces cerevisiae, Folia Microbiol. (Prague), 38, 59, 10.1007/BF02814551 Wada, 1990, The SLP1 gene of Saccharomyces cerevisiae is essential for vacuolar morphogenesis and function, Molecular and Cellular Biology, 10, 2214, 10.1128/MCB.10.5.2214 Walther, 1989, Localization of carbamyl-phosphate-metabolizing enzymes in different organelles within tomato (Lycopersicon esculentum) cells, Biochem. Physiol. Pflanzen., 185, 201, 10.1016/S0015-3796(89)80081-2 Wright, 1989, Transmission electron microscopy and immunochemical studies of yeast: analysis of HMG-CoA reductase overproduction by electron microscopy, Methods in Cell Biology, 31, 473, 10.1016/S0091-679X(08)61624-6 Yotsuanagi, 1988, Fibrous component of yeast mitochondria, J. Ultrastruct. Res., 98, 254, 10.1016/S0889-1605(88)80918-2