Andre T Baron1, Tammy M Greenwood1, Christopher W Bazinet2, Jeffrey L Salisbury1
1Laboratory for Cell Biology, Department of Biochemistry and Molecular Biology, Mayo Clinic Foundation, Rochester, Minnesota 55905 USA
2Case Western Reserve University, Department of Genetics, Cleveland, Ohio 44106,USA
Tóm tắt
Summary— Here, we use three polyclonal anticentrin antisera designated 08/28, 26/14‐1, and 26/14‐2 to further characterize the pericentriolar lattice of metazoan cells. all of these antibodies give an indistinguishable localization pattern that consists of a constellation of pericentrosomal spots. In QT6 cells these spots are few in number and closely associated with the centriolar region, whereas in PtK2 cells they are more numerous and distributed further from the point of microtubule focus. In mitotic cells, centrin is localized to the spindle poles and spindle apparatus. We demonstrate here that the pericentriolar lattice of PtK2 and QT6 cells is, in part, composed of proteins characterized by acidic pIs (4.4 to 5.4), low molecular mass (Mr 18 500–21000), and calcium‐binding; these attributes and the immunoreactivity of these proteins to anticentrin antibodies indicate that they are centrin isoforms of metazoan cells. Finally, we confirm our earlier observation that PtK2 cells contain a centrin‐related protein of Mr 165000; QT6 cells also contain centrin‐related proteins (Mr 64000–165000). We conclude that centrin is a component of the pericentriolar lattice of higher eukaryotic centrosomes.
