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Biểu hiện cụ thể theo loại tế bào và sự khác biệt của RNA không mã hóa dài LINC-RSAS giảm trong tinh hoàn trong quá trình lão hóa của chuột
Biogerontology - Trang 1-24 - 2024
Tóm tắt
RNA không mã hóa dài (lncRNAs) đã nổi lên như những chất điều tiết chính của biểu hiện gen, cấu trúc nhiễm sắc, các thay đổi di truyền, xử lý sau phiên mã của RNA, dịch mã mRNA thành protein, cũng như đóng góp vào quá trình lão hóa. Lão hóa là sự thay đổi chậm, tiến triển và phổ quát trong hầu hết các quá trình sinh lý của sinh vật sau khi đạt được sự trưởng thành sinh sản, thường liên quan đến các bệnh liên quan đến tuổi tác. Tinh hoàn của động vật có vú chứa nhiều loại tế bào khác nhau, là kho chứa sự phức tạp của bản sao, sản xuất giao tử nam đơn bội cho sinh sản và testosterone cho sự phát triển và duy trì các đặc điểm sinh dục nam, cũng như đóng góp vào sự biến đổi di truyền của loài. Chúng tôi báo cáo sự giảm biểu hiện và vị trí tế bào của RNA Long intergenic noncoding repeat-rich sense-antisense (LINC-RSAS) có liên quan đến tuổi tác trong tinh hoàn và các loại tế bào chính như tinh bào sơ cấp, tế bào Leydig và tế bào Sertoli trong quá trình lão hóa của chuột. Biểu hiện LINC-RSAS trong tinh hoàn tăng từ giai đoạn chưa trưởng thành (4 tuần) đến giai đoạn trưởng thành (16 và 44 tuần) và giảm từ giai đoạn trưởng thành (44 tuần) đến gần tuổi già (70 tuần). Sự metyl hóa DNA gen trong tinh hoàn cho thấy mô hình tương tự. Biểu hiện LINC-RSAS cao hơn cụ thể theo loại tế bào được quan sát thấy ở tinh bào sơ cấp (các tế bào pachytene), tế bào Leydig và tế bào Sertoli của tinh hoàn chuột trưởng thành. Việc quá biểu hiện LINC-RSAS trong các dòng tế bào người nuôi cấy cho thấy vai trò khả thi của nó trong việc kiểm soát chu kỳ tế bào và chết tế bào. Chúng tôi đề xuất rằng biểu hiện LINC-RSAS có liên quan đến sinh lý phân tử của tinh bào sơ cấp, tế bào Leydig và tế bào Sertoli trong tinh hoàn trưởng thành và sự giảm sút của nó có liên quan đến sự suy giảm chức năng của tinh hoàn trong quá trình lão hóa của chuột.
Từ khóa
#Long noncoding RNAs #LINC-RSAS #tế bào Leydig #tế bào Sertoli #lão hóa #tinh hoàn #di truyền họcTài liệu tham khảo
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