Cell proliferation at different sites along the length of the rat colon
Tóm tắt
This study has been undertaken in order to compare in detail cell proliferation in the mucosal crypts at several sites along the length of the large bowel of the rat. The techniques which have been used include simple morphometry, calculation of mitotic and tritiated thymidine labelling indices, metaphase arrest with vincristine, and the fraction of labelled mitoses method. Major differences exist in the size and shape of the crypts at different sites. In particular the distribution of the proliferating cells within the crypt varies. Mean cell cycle time ranges from 58 h in the descending colon to 25 h in the caecum; this variation appears to be brought about largely by changes in the duration of G1, the other phase durations remaining relatively constant. There is also variation in cell cycle time and growth fraction at different levels within the crypt; throughout the bowel cells appear to cycle more slowly at the bottom of the crypt, but changes in growth fraction do not display a similarly consistent pattern. Clearly the organisation of cell proliferation in the normal rat colon is very complex, and strict definition of anatomical location is required in any study of cell proliferation whether in normal or in diseased animals.
Tài liệu tham khảo
Appleton, D.R., Sunter, J.P.: Estimating the proportion of proliferating cells in a population. Virchows Arch. B Cell Path.32, 69–73 (1979)
Appleton, D.R., Sunter, J.P., Watson A.J.: The cut-off position in the intestinal crypt. Cell Tiss. Kinet. (submitted)
Cairnie, A.B., Lamerton, L.F., Steel, G.G.: Cell proliferation studies in the intestinal epithelium of the rat: I. determination of the kinetic parameters. Exp. Cell Res.39, 528–538 (1965)
Cairnie, A.B., Bentley, J.: Cell proliferation studies in the intestinal epithelium of the rat: Hyperplasia during lactation. Exp. Cell Res.46, 428–440 (1967)
Cleaver, J.E.: Thymidine metabolism and cell kinetics. Amsterdam: North Holland 1967
Gilbert, C.W.: The labelled mitoses curve and the estimation of the parameters of the cell cycle. Cell Tiss. Kinet.5, 53–63 (1972)
Hamilton, E.: Diurnal variation in proliferative compartments and their relation to cryptogenic cells in the mouse. Cell Tiss. Kinet.12, 91–100 (1979)
Messier, B., Leblond, C.P.: Cell proliferation and migration as revealed by radioautography after injection of Thymidine H3 into male rats and mice: Am. J. Anat.106, 247–265 (1960)
Reddy, B.S., Narisawa, T., Weisburger, J.H.: Effect of a diet with high levels of protein and fat on colon carcinogenesis in R344 rats treated with 1,2-dimethylhydrazine. J. Natl. Cancer Inst.57, 567–569 (1976)
Rijke, R.P.C., Plaisier, H.M., Langendoen, N.J.: Epithelial cell kinetics in the descending colon of the rat. Virchows Arch. B Cell Path.30, 85–94 (1979)
Rodriguez, M.S.B. de., Sunter, J.P., Watson, A.J., Wright, N.A., Appleton, D.R.: Cell population kinetics in the mucosal crypts of the descending colon of the mouse. Virchows Arch. B Cell Path.29, 351–361 (1979)
Steel, G.G.: Growth kinetics of tumours. Oxford: Oxford University Press 1977
Sunter, J.P., Appleton, D.R., Wright, N.A., Watson, A.J.: Pathological features of the colonic tumours induced in rats by the administration of 1,2-dimethylhydrazine. Virchows Arch. B Cell Path.29, 211–223(1978)
Sunter, J.P., Wright, N.A., Appleton, D.R.: Cell population kinetics in the epithelium of the colon of the male rat. Virchows Arch. B Cell Path.26, 275–287 (1978)
Sunter, J.P., Appleton, D.R., de Rodriguez, M.S.B., Wright, N.A., Watson, A.J.: A comparison of cell proliferation at different sites within the large bowel of the mouse. J. Anat. (in press)
Tannock, I.F.: A comparison of the ralative efficiencies of various metaphase arrest agents. Exp. Cell Res.47, 345–356 (1976)
Tutton, P.J.M., Barkla, D.H.: Cell proliferation in the descending colon of dimethylhydrazine treated rats and in dimethylhydrazine induced adenocarcinomata. Virchows Arch. B Cell Path.21, 147–160(1976)
Ward, J.M.: Morphogenesis of chemically induced neoplasms of the colon and small intestine in rats. Lab. Invest.30, 505–513 (1974)
Wiebecke, B., Krey, U., Lohrs, U., Eder, M.: Morphological and autoradiographical investigations on experimental carcinogenesis and polyp development in the intestinal tract of rats and mice. Virchows Arch. Abt. A Path. Anat.360, 179–193 (1973)
Wright, N.A., Morley, A.R., Appleton, D.R.: Variation in the duration of mitosis in the crypts of Lieberkuhn in the rat: a cytokinetic study using vincristine. Cell Tiss. Kinet.5, 351–364 (1972)