Các mối nối tế bào và giao tiếp liên tế bào

In Vitro Cellular & Developmental Biology - Plant - Tập 16 - Trang 1010-1017 - 1980
J. -P. Revel1, S. B. Yancey1, D. J. Meyer1, B. Nicholson1
1Division of Biology, California Institute of Technology, Pasadena

Tóm tắt

Chúng tôi đã so sánh giao tiếp tế bào trong gan chuột bình thường và gan đang tái tạo. Các mối nối khe (gap junctions) bị giảm đáng kể về kích thước và số lượng từ 29 đến 35 giờ sau phẫu thuật cắt gan (hepatectomy), nhưng chúng tôi vẫn tìm thấy khoảng 90% tế bào gan (hepatocyte) được kết nối theo các tiêu chí sinh lý điện. Tuy nhiên, sự lan truyền của các thuốc nhuộm như carboxyfluorescein thì rất hạn chế trong các cơ quan đang tái tạo so với tình trạng ở nhóm kiểm soát. Chúng tôi cho thấy cách hòa giải những khác biệt rõ ràng giữa dữ liệu hình thái học và sinh lý học. Chúng tôi cũng trình bày một tóm tắt về các phát hiện ban đầu về sinh tổng hợp protein mối nối khe và một số kết luận có thể rút ra từ chuỗi 58 amino acid tại đầu amino của protein.

Từ khóa

#giao tiếp liên tế bào #mối nối khe #gan chuột #sinh tổng hợp protein #tế bào gan

Tài liệu tham khảo

Dewey, M. M.; Barr, L. Intercellular connections between smooth muscle cells. The nexus. Science 137: 670–672; 1962. Simionescu, M.; Simionescu, N.; Palade, G. Segmental variations of cell junctions in the vascular endothelium: the microvasculature. J. Cell Biol. 67: 863–885; 1975. De Haan, R. L.; Hirakow, R. Synchronization of pulsation rates in isolated cardiac myocytes. Exp. Cell Res. 70: 214–220; 1972. Payton, B. W.; Bennett, M. V. L.; Pappas, G. D. Permeability and structure of junctional membranes at an electronic synapse. Science 166: 1641–1643; 1969. Revel, J. P.; Karnovsky, M. J. Hexagonal array of subunits in intercellular junctions of the mouse heart and liver. J. Cell Biol. 33: C7-C12; 1967. Gilula, N. B.; Reeves, R. O.; Steinbach, A. Metabolic coupling, ionic coupling and cell contacts. Nature 235: 262–265; 1972. Loewenstein, W. R. Permeability of membrane junctions. Ann. N.Y. Acad. Sci. 137: 441–472; 1966. Potter, D. D.; Furshpan, E. J.; Lennox, E. S. Connections between cells of the developing squid as revealed by electrophysiological methods. Proc. Natl. Acad. Sci. USA 55: 328–335; 1966. Revel, J. P.; Yee, A. G.; Hudspeth, A. J. Gap junctions between electrotonically-coupled cells in tissue culture and in brown fat. Proc. Natl. Acad. Sci. USA 68: 2924–2927; 1971. Azarnia, R.; Michalke, W.; Loewenstein, W. R. Intercellular communication and tissue growth. VI. Failure of exchange of endogeneous molecules between cancer cells with defective junctions and noncancerous cells. J. Membr. Biol. 10: 247–258; 1972. Azarnia, R.; Larsen, W. J. Intercellular communication and cancer. De Mello, ed. Intercellular communication. New York: Plenum; 1977; 145–172. Bennett, M. V. L. Function of electrotonic junctions in embryonic and adult tissues. Fed. Proc. 32: 65–75; 1973. Bennett, M. V. L.; Goodenough, D. A. Gap junctions, electrotonic coupling and intercellular communication. Neurosci. Res. Progra. Bull. 16: 373–486; 1978. Griepp, E. B.; Revel, J. P. Gap junctions in development. De Mello, W. C. ed. Intercellular communication. New York: Plenum; 1977; 1. Loewenstein, W. R. Membrane junctions in growth and differentiation. Fed. Proc. 32: 60–64; 1973. Loewenstein, W. R. Intercellular communication in normal and neoplastic tissues. Schultz and Block, eds. Membrane transformation in neoplasia. New York: Academic Press; 1974: 239–248. Sheridan, J. D. Electrical coupling of cells and cell communication. Cox, R. P. ed. Cell communication. New York; J. Wiley and Sons; 1974: 31–32. Sheridan, J. D. Low resistance junctions: some functional considerations. Cox, R. E. ed. The cell surface in development. New York: Wiley; 1974: 31. Weber, G. Biochemical strategy of the regenerating liver cell. Lesch, R.; Rentter, W. eds., Liver regeneration after experimental injury. New York: Stratton Intercontinental Medical Book Corp. 1975: 103–117. Caspar, D. L. D.; Goodenough, D. A.; Makowski, L.; Phillip, W. E. Gap junction structures. I. Correlated electron microscopy and X-ray diffraction. J. Cell. Biol. 74: 605–628; 1977. Makowski, L.; Caspar, D. L. D.; Phillips, W. C.; Goodenough, D. A. Gap junction structures. II. Analysis of the X-ray diffraction data. J. Cell Biol. 74: 629–644; 1977. Unwin, P. N. T.; Zampighi, G. Structure of the junction between communicating cells. Nature 283: 545–549; 1980. Flagg-Newton, J.; Simpson, I.; Loewenstein, W. R. Permeability of the cell-cell membrane channels in mammalian cell junction. Science 205: 404–407; 1979. Lawrence, T. S.; Beers, W. H.; Gilula, N. B. Transmission of hormonal stimulation by cell-to-cell communication. Nature 272: 501–506; 1978. Pitts, J. D.; Finbow, M. E. Junctional permeability and its consequences. De Mello, W. C. ed. Intercellular communication. New York: Plenum; 1977: 61–86. De Mello, W. C. Effect of intracellular injection of calcium and strontium on cell communication in heart. J. Physiol. (Lond.) 250: 231–245; 1975. Rose, B.; Rick, R. Intracellular pH, intracellular free Ca, and junctional cell-cell coupling. J. Membr. Biol. 44: 377–415; 1978. Rose, B.; Simpson, I.; Loewenstein, W. R. Calcium ion produces graded changes in permeability of membrane channels in cell junction. Nature 267: 625–627; 1977. Spray, D. C.; Harris, A. L.; Bennett, M. V. L. Voltage dependence of junctional conductance in early amphibian embryos. Science 204: 432–434; 1979. Turin, L.; Warner, A. Carbon dioxide reversibly abolishes ionic communication between cells of early amphibian embryo. Nature 270: 56–57; 1977. Baldwin, K. Cardiac gap junction configuration after an uncoupling treatment as a function of time. J. Cell Biol. 82: 66–75; 1979. Dahl, G.; Isenberg, G. Nexus ultrastructure in decoupled heart cells. J. Membr. Biol. 53: 63–75; 1980. Pappas, G. D.; Asada, Y.; Bennett, M.V. L. Morphological correlates of increased coupling resistance at an electronic synpase. J. Cell Biol. 49: 173–188; 1971. Peracchia, C.; Dulhunty, A. Low resistance junctions in crayfish. Structural changes with functional uncoupling. J. Cell Biol. 70: 419–439; 1976. Paracchia, C. Gap junctions. Structural changes after uncoupling. J. Cell Biol. 72: 628–645; 1977. Raviola, E.; Goodenough, D. A.; Raviola, G. The native structure of gap junctions rapidly frozen at 4°C. J. Cell Biol. 79: 229a; 1978. Decker, R. S.; Hormonal regulation of gap junction differentiation. J. Cell Biol. 69: 669–685; 1976. Decker, R. S.; Friend, D. S. Assembly of gap junctions during amphibian neurulation. J. Cell Biol. 62: 32–47; 1974. Ducibella, J.; Albertini, D.; Anderson, E.; Biggers, J. D. The preimplantation mammalian embryo: characterization of intercellular junctions and their appearance during development. Dev. Biol. 47: 231–250; 1975. Fallon, J. F.; Kelley, R. O. Ultrastructural analysis of the apical ectodermal ridge during vertebrate limb morphogenesis. J. Embryol. Exp. Morphol. 41: 223–232; 1977. Gilula, N. B.; Epstein, M. L.; Beers, W. H. Cell-to-cell communication and ovulation. A study of the cumulus-oocyte complex. J. Cell Biol. 78: 58–78; 1978. Ginsberg, R. D.; Gilula, N. B. Modulation of cell junctions during differentiation of the chicken otocyst sensory epithelium. Dev. Biol. 68: 110–129; 1979. Gros, D.; Mocquard, J. P.; Challice, C. E.; Schrevel, J. Formation and growth of gap junctions in mouse myocardium during ontogenesis: a freeze-cleave study. J. Cell. Sci. 30: 45–61; 1978. Kalderon, N.; Epstein, M. L.; Gilula, N. B. Cell-cell communication and myogenesis. J. Cell Biol. 75: 788–806; 1977. Lo, C. W.; Gilula, N. B. Gap junctional communication in the postimplantation mouse embryo. Cell 18: 411–422; 1979. McNutt, N. S.; Weinstein, R. S. Carcinoma of the cervix. Deficiency of nexus intercellular junctions. Science 165: 597–599; 1969 Powers, R. D.; Tupper, J. T. Intercellular communication in the early embryo. De Mello, W. C. ed. Intercellular communication. New York: Plenum; 1978: 231–251. Rash, J. E.; Staehelin, L. A. Freeze cleave demon-stration of gap junctions between skeletal myogenic cells in vivo. Dev. Biol. 36: 455–561; 1974. Revel, J. P.; Brown, S. S. cell junctions in development, with particular reference to the neural tube. Cold Spring Harbor Symp. Quant. Biol. 40; 443–455; 1976. Slack, C.; Palmer, J. F. The permeability of intercellular junctionsin the early embryo ofXenopus laevis studies with a fluorescent tracer. Exp. cell Res. 55: 416–419; 1969. Wolpert, L. Gap junctions: channels for communication in developoment. Feldman, J.; Gilula, N. B.; Pitts, J. D. eds. Intercellular junctions and synapses. London; Chapman and Hall; 1978: 83–94. Lawrence, P. A.; Green, S. M. The anatomy of a compartment border. The intersegmental boundary in Oncopeltus. J. Cell Biol. 65: 373–382; 1975. Yancey, S. B.; Easter, D.; Revel, J. P. Cytological changes in gap junctions during liver regeneration. J. Ultrastruct. Res. 67: 229–242; 1979. Yee, A. G.; Revel, J. P. Loss and reappearance of gap junctions in regenerating livers. J. Cell Biol. 78: 544–564. Finbow, M.; Yancey, S. B.; Johnson, R.; Revel, J. P. Independent lines of evidence suggesting a major gap junctional protein with a molecular weight of 26,000. Proc. Natl. Acad. Sci. USA 77: 970–974; 1980. Graf, J.; Petersen, O. H. Cell membrane potential and resistance in liver. J. Physiol. (Lond.) 284: 105–126; 1978. Loewenstein, W. R.; Penn, R. D. Intercellular communication and tissue growth. II. Tissue regeneration. J. Cell Biol. 33: 235–242; 1967. Meyer, D. J.; Yancey, S. B.; Revel, J. P. Inter-cellular communication in regenerating rat liver. Submitted for publication. Penn, R. D. Ionic communication between liver cells. J. Cell Biol. 29: 171–174; 1966. Culvenor, J. G.; Evans, W. H. Preparation of hepatic gap (communicating) junctions. Identification of the constituent polypeptide subunits. Biochem. J. 168: 475–481; 1977. Duguid, J. R. Investigations into the structure of the murine hepatocyte gap junction. Pasadena: California Institute of Technology; 1975. Thesis. Duguid, J.; Revel, J. P. The protein components of the gap junction. Cold Spring Harbor Symp. Quant. Biol. 40: 45–47; 1976. Ehrhart, J. C.; Chauveau, J. The protein component of mouse hepatocyte gap junctions. FEBS Lett. 78: 295–299; 1977. Goodenough, D. A. In vitro formation of gap junction vesicles. J. Cell Biol. 68: 220–231; 1976. Goodenough, D. A.; Stoeckenius, W. The isolation of mouse hepatocyte gap junctions. Preliminary chemical characterization and X-ray diffraction. J. Cell Biol. 54: 646–656; 1972. Henderson, D.; Eibl, H.; Weber, K. Structure and biochemistry of mouse hepatic gap junctions. J. Mol. Biol. 132: 193–218; 1979. Herzberg, E. L.; Gilula, N. B. Isolation and characterization of gap junctions from rat liver. J. Biol. Chem. 254; 2138–2147; 1979. Nicholson, B. J.; Hunkapiller, M. W.; Hood, L. E.; Revel, J. P.; Takemoto, L. 1980. Partial sequencing of the gap junctional protein from rat lens and liver, submitted for the 20th Annual Meeting of the American Society for Cell Biology. Gurd, F.; Evans, W. H. Relative rates of degradation of mouse-liver surface-membrane proteins. Eur. J. Biochem. 36: 273–279; 1973. Griepp, E. B.; Bernfield, M. Acquisition of synchronous beating between embryonic heart cell aggregates and layers. Exp. Cell Res. 113: 263–272; 1978. Williams, E. H.; De Haan, R. L. Alteration in synchronization time of newly apposed heart cell aggregates by pretreatment with trypsin and cycloheximide. J. Cell Biol. 79: 239a; 1978. Epstein, M.; Sheridan, J.; Johnson, R. J. Formation of low-resistance junctions in vitro in the absence of protein synthesis and ATP production. Exp. Cell Res. 104: 25–30; 1977. Shimada, Y.; Moscona, A. A.; Fischman, D. A. Scanning electron microscopy of cell aggregation. Dev. Biol. 36: 428–446; 1974. Rabes, H. M. Kinetics of hepatocellular proliferation as a function of the microvascular structure and functional state of the liver. Porter, R.; Whelan, J. eds. Hepatotrophic Medica; North Holland; J. Elservier Excerpta Medica; 1978. O'Lague, P.; Dalen, H.; Rubin, H.; Tobias, C. Electrical coupling: low resistance junctions between mitotic and interphase fibroblasts in tissue culture. Science 170: 464–466; 1970. Scornik, O. A. In vivo synthesis, export and degradation of proteins in the regenerating mouse liver. Lesch, R.; Rentter, W. eds. Liver regeneration after experimental injury. New York: Stratton Intercontinental Medical Book Corp; 1975: 156–167.